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Front. Pharmacol. | doi: 10.3389/fphar.2019.01272

Antiviral Activity against Infectious Bronchitis Virus and Bioactive Components of Hypericum Perforatum L.

 Huijie Chen1,  Ishfaq Muhammad1, Yue Zhang1, Yudong Ren1, Ruili Zhang1, Xiaodan Huang1, Lei Diao2, Haixin Liu1, Xunliang Li1, Xiaoqi Sun1,  Ghulam Abbas1 and Guangxing Li1*
  • 1College of Veterinary Medicine, Northeast Agricultural University, China
  • 2Jilin Agricultural Science and Technology College, China

Hypericum perforatum L., also known as Saint John’s Wort, has been well studied for its chemical composition and pharmacological activity. In this study, the antiviral activities of hypericum perforatum L. on infectious bronchitis virus (IBV) were evaluated in vitro and in vivo for the first time. The results of in vitro experiments confirmed that the antiviral component of hypericum perforatum L. was ethyl acetate extraction section (HPE), and results showed that treatment with HPE significantly reduced the relative mRNA expression and virus titer of IBV, and reduced positive green immunofluorescence signal of IBV in chicken embryo kidney (CEK) cells. HPE treatment at doses of 480-120 mg/kg for 5 days, reduced IBV induced injury in the trachea and kidney, moreover, reduced the mRNA expression level of IBV in the trachea and kidney in vivo. The mRNA expression levels of IL-6, TNF-α, and NF-κB significantly decreased, but MDA5, MAVS, IFN-α and IFN-β mRNA levels significantly increased in vitro and in vivo. Our findings demonstrated that HPE had significant anti-IBV effects in vitro and in vivo, respectively. In addition, it is possible owing to up-regulate mRNA expression of type I interferon through the MDA5 signaling pathway and down-regulate mRNA expression of IL-6 and TNF-α via the NF-κB signaling pathway. Moreover, the mainly active compositions of HPE analyzed by HPLC/ESI-MS are hyperoside, quercitrin, quercetin, pseudohypericin and hypericin, and a combination of these compounds could mediate the antiviral activities. This might accelerate our understanding of the antiviral effect of hypericum perforatum L. and provide new insights into the development of effective therapeutic strategies.

Keywords: Hypericum perforatum L., Infectious bronchitis virus, antiviral, MDA5, NF-κB, HPLC/ESI-MS

Received: 13 May 2019; Accepted: 04 Oct 2019.

Copyright: © 2019 Chen, Muhammad, Zhang, Ren, Zhang, Huang, Diao, Liu, Li, Sun, Abbas and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Guangxing Li, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150038, Heilongjiang Province, China,