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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Psychiatry | doi: 10.3389/fpsyt.2019.00614

Serum microRNAs in ASD: association with monocyte cytokine profiles and mitochondrial respiration

  • 1Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, United States
  • 2Saint Peter's University Hospital, United States
  • 3University of Texas MD Anderson Cancer Center, United States
  • 4Arkansas Children's Research Institute (ACRI), United States
  • 5Phoenix Children's Hospital, United States

Our previous research has shown that purified peripheral blood monocytes (PRMo) from individuals who are diagnosed with autism spectrum disorders (ASD) and have innate immune abnormalities reveal altered interleukin-1ß (IL-1ß)/IL-10 ratios. We also found, in separate studies, that microRNA (miRNA) expression in PBMo and mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) differed in the IL-1ß/IL-10 based ASD subgroups. This study explored whether serum miRNAs are associated with both altered innate immune responses and changes in mitochondrial respiration as a link of regulatory mechanisms for these two common abnormalities in ASD subjects.
Serum miRNA levels were examined by high throughput deep sequencing in ASD and non-ASD control sera with concurrent measurement of PBMo cytokine production and mitochondrial respiration by PBMCs. ASD samples were examined as a whole group and with respect to the previously defined IL-1ß/IL-10 based ASD subgroups (high, normal, and low groups)(Jyonouchi et al., 2017b).
Serum miRNA levels differed between the overall ASD sera (N=116) and non-ASD control sera (N=35) and also differed across the IL-1ß/IL-10 based ASD subgroups. Specifically, miRNA levels were increased and decreased in 8 and 9 miRNAs, respectively, in the high ratio ASD subgroup (N=48). In contrast, the low (N=25) and normal (N=43) ratio ASD subgroups, only showed decreased miRNAs levels (18, and 10 miRNAs, respectively). Gene targets of the altered miRNAs in the high and/or low IL-1β/IL-10 ratio ASD subgroups were associated with pathways critical for monocyte functions and metabolic regulation. Gene targets of the altered miRNAs in all the ASD subgroups were associated with pathways of neuronal development and synaptic plasticity, along with cell proliferation/differentiation. ASD subgroup-specific associations were observed between serum miRNA expression and IL-1ß/IL-10 ratios, mitochondrial respiration, and monocyte cytokine profiles (IL-10, CCL2, and TNF-α). In summary, our results indicate that serum levels of select miRNAs may serve as promising biomarkers for screening and monitoring changes in innate immunity and mitochondrial respiration in ASD.

Keywords: ASD, cytokine, Mitocondria, microRNA, Monocytes, Serum

Received: 14 Mar 2019; Accepted: 01 Aug 2019.

Edited by:

Chad A. Bousman, University of Calgary, Canada

Reviewed by:

Kurt L. Hoffman, Autonomous University of Tlaxcala, Mexico
Roberto F. De Almeida, Universidade Federal de Ouro Preto, Brazil  

Copyright: © 2019 Jyonouchi, Geng, Toruner, Rose, Bennuri and Frye. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Harumi Jyonouchi, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, New Brunswick, United States, hjyonouchi@gmail.com