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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Public Health | doi: 10.3389/fpubh.2019.00348

DIARRHOEAGENIC ESCHERICHIA COLI PATHOTYPES FROM CHILDREN YOUNGER THAN FIVE YEARS IN KANO STATE, NIGERIA

 Habeeb K. Saka1*, Nasir T. Dabo1, Bashir Muhammad1,  Silvia Garcia-soto2,  María Ugarte-Ruiz2 and  Julio Alvarez2
  • 1Bayero University Kano, Nigeria
  • 2VISAVET Health Surveillance Centre (UCM), Spain

Diarrhoeagenic Escherichia coli (DEC) is one of the leading cause of gastrointestinal disorders worldwide and an important public health challenge. It is often underdiagnosed during routine microbiological analysis, especially in resource constrained settings. The use of molecular tests could however help to determine the distribution of DEC and their clinical significance. Here, a study to assess the prevalence of DEC in clinical samples from patients <5 years attending three hospitals in Kano state, Nigeria was carried out. Samples from 400 patients and 50 controls were collected and screened for E. coli. Compatible colonies from 248 individuals (215 patients and 33 controls) were characterized using biochemical test, real-time PCR for detection of nine virulence factors (VF: eae, bfpA, elt, est, stx1, stx2, ehxA, aggR and invA) associated with five DEC pathotypes (EPEC, ETEC, EHEC, EAEC and EIEC) and antimicrobial susceptibility tests. One or more VFs typical to a pathotype were detected in 73.8% (183/248) of the isolates, with those associated with EAEC(36.3%), ETEC(17.3%) and EPEC(6.0%) being the most common, although proportion of specific pathotypes differ between hospitals. est was the only VF detected in a significantly higher proportion in cases compared to controls (P-value=0.034). Up to 86.9% of DEC were resistant to at least one class of antibiotics, with trimethoprim-sulfamethoxazole being the least effective drug (77.6% resistance). Our results demonstrate widespread circulation of different DEC pathotypes among children in Kano state that were highly resistant to trimethoprim-sulfamethoxazole, and highlight the need of characterizing the causative agents in cases of gastrointestinal disorders.

Keywords: Diarrhoegenic Escherichia coli, Virulence Factors, Pathotypes, Resistance, Kano state

Received: 17 Jul 2019; Accepted: 01 Nov 2019.

Copyright: © 2019 Saka, Dabo, Muhammad, Garcia-soto, Ugarte-Ruiz and Alvarez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mr. Habeeb K. Saka, Bayero University Kano, Kano, Nigeria, sakakayodehabeeb@gmail.com