Frontiers in Bioengineering and Biotechnology

Original Research

Accepted on 23 Jun 2025

Hyaluronidases improve the hyaluronic acid yield during the fermentation of Streptococcus zooepidemicus

in Bioprocess Engineering

Chuan-Li Kang
De-Qiang Zheng
Zhi-Yuan Yao
Kang Yang
Yuxue Zhao
Zihan Mao
Yang Liu
Haijun Li
Jin-Song Gong
Lei Liu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1625009

Original Research

Accepted on 16 Jun 2025

Cell Specific Perfusion Rates Drive Growth Dynamics and Metabolism in CHO N-1 Perfusion Processes Independent of Perfusion Rate Control Method

in Bioprocess Engineering

Julia Walther
Tiago Ribeiro Da Costa
Lydia Winkler
Jochen Schaub
Tobias Habicher

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1608889

152 views

Review

Accepted on 12 Jun 2025

Recent Advances and Future Directions on GLA-Producing Organisms

in Bioprocess Engineering

Foroozan Jalali Bidgoli
Helia Hajihassani
Mojgan Latifi
Danial Hassani
Pär Ingvarsson
Naser Farrokhi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1567840

115 views

Original Research

Accepted on 06 Jun 2025

Automated, aseptic sampling with small-volume capacity from microbioreactors for cell therapy process analysis

in Bioprocess Engineering

Zhi Xian Chan
Shruthi Pandi Chelvam
Wei-Xiang Sin
Denise Bei Lin Teo
Ahmad Amirul Abdul Rahim
Ying Ying Wu
Dan Liu
Michael Birnbaum
Derrick Yong
Rajeev Ram

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1612648

434 views

Brief Research Report

Published on 05 Jun 2025

Unlocking efficiency in column chromatography with packed bed supporting inserts

in Bioprocess Engineering

Romone M. Fancy
David H. Abraham
Matthew R. Taylor
Cole McMullin
Nicholas S. Brann
Daniel M. Bailey
David I. Brown
Heather Bethea Horne
Leslie S. Wolfe

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1613174

1,188 views

Review

Published on 04 Jun 2025

Enzymatic approaches to nicotinic acid synthesis: recent advances and future prospects

in Bioprocess Engineering

Rahul Vikram Singh
Vipin Chandra Kalia
Krishika Sambyal
Bakul Singh
Anurag Kumar
Jung-Kul Lee

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1585736

2,671 views

Methods

Accepted on 03 Jun 2025

Optimization of protein extraction from growing antlers of deer

in Bioprocess Engineering

Nicolás Alegría-Aravena
María Pilar López Garrido
Josefa Antonia Quiroz Troncoso
Raquel González-Martos
Marta Sánchez Díez
Clara Elizabeth Gavira-O'Neill
Andrés J García-Díaz
Tomás Landete-Castillejos
Carmen Ramirez-Castillejo
Louis Chonco

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1612239

297 views

Original Research

Published on 02 Jun 2025

Optimising and adapting perfusion feeds in serum-free medium to intensify CAR-T cell expansion in stirred-tank bioreactors

in Bioprocess Engineering

Pierre Springuel
Tiffany Hood
Fern Slingsby
Timo Schmidberger
Nicola Bevan
Noushin Dianat
Julia Hengst
Qasim A. Rafiq

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1593895

1,672 views

Original Research

Published on 30 May 2025

Accuracy and cost comparison of 3D-printed guides in complex spinal deformity correction: direct vs indirect design

in Bioprocess Engineering

Wei Yang
Wei Guo
Wen-Jun Wu
Rong Ma
Zemin Wang
Honglai Zhang
Wanzhong Yang
Zhaohui Ge

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1611998

830 views

Original Research

Published on 30 May 2025

Perfusion process with tangential flow filtration for oncolytic VSV-GP production

in Bioprocess Engineering

Orsolya Hamusics
Anja Wittmann
Katrin Hasler
Sabrina Müller
Daniel Birk
Ingo H. Gorr
Alexander Brix
Jorge Soza-Ried

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1588293

1,088 views

Original Research

Accepted on 26 May 2025

Design, Synthesis, and Anti-inflammatory Potential of PROTAC Drug Molecules Based on Fondaparinux Sodium

in Bioprocess Engineering

Ruoxuan Wu
Tianji Zhang
Siran Zhao
Marco Maccarana
Jin-ping Li
Hui Cao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1597344

404 views

Original Research

Published on 21 May 2025

mAb production kinetics in CHO batch culture: exploring extracellular and intracellular dynamics

in Bioprocess Engineering

Alejandro Avilan Garzon
Bruno Ebel
Cédric Paris
Samuel Schneider
David Pfister
Eric Olmos

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1546105

1,641 views

Review

Published on 12 May 2025

Shaping the future of tobacco through microbial insights: a review of advances and applications

in Bioprocess Engineering

Wei Hu
Jiaxing Yuan
Jiaxiang Fei
Kaleem Imdad
Pengfei Yang
Shen Huang
Duobin Mao
Jing Yang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1548323

943 views

Original Research

Published on 02 May 2025

New insights on poly(cis-1,4-isoprene) rubber degradation through enzymatic kinetics and process improvement

in Bioprocess Engineering

Camila Guajardo-Flores
Josefa Rojas
Yvan Baldera-Moreno
Francisco Adasme-Carreño
Daisuke Kasai
Rodrigo Andler

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1593339

1,350 views

Original Research

Published on 30 Apr 2025

Intensification of 2′-Fucosyllactose biosynthesis pathway by using a novel fucosyltransferase from Bacillus cereus

in Bioprocess Engineering

Kainuo Zhang
Miaomiao Gao
Chenqi Cao
Mengxin Zhang
Waqar Ahmad
Ahmed Rady
Badr Aldahmash
Tianze Zhu
Shahin Shah Khan
Luo Liu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1569597

1,416 views

Original Research

Published on 29 Apr 2025

Scalability of spheroid-derived small extracellular vesicles production in stirred systems

in Bioprocess Engineering

Thibaud Dauphin
Laurence de Beaurepaire
Apolline Salama
Quentin Pruvost
Clémentine Claire
Karine Haurogné
Sophie Sourice
Aurélien Dupont
Jean-Marie Bach
Julie Hervé

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1516482

1,683 views