Frontiers in Bioengineering and Biotechnology

Original Research

Published on 30 Jan 2026

Towards a cleaner CHO chassis: systematic knockout of host cell proteins for efficient biopharmaceutical manufacturing

in Bioprocess Engineering

Jannis Peter Marzluf
Jennifer Klein
Daniela Kirchmeier
Alexander Stärk
Daniel Ziegner
Markus Gehrung
Franziska Rauh
Merle Rattay
Christoph Zehe
Ann-Cathrin Leroux

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2026.1750646

643 views

Review

Accepted on 21 Jan 2026

Structural Improvement Towards the Efficiency of Biodigesters in the 21st Century: A Review of the Different Designs

in Bioprocess Engineering

Takalani Nethavhanani
Vhutshilo 1st Mountaineer Nekhubvi
Lutendo Michael Mathomu
Nnditshedzeni Eric Maluta

Frontiers in Bioengineering and Biotechnology

137 views

Original Research

Published on 21 Jan 2026

Whole-genome DNA methylation analysis of Chinese hamster ovary cells undergoing media adaptation

in Bioprocess Engineering

Suki Roy
Jasrene Kaur Sandhu
Lingzhi Huang
Abraham Wong
Guo Xuan Wan
Frank Lyko
Emeka Ignatius Igwe
Florian Böhl

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2026.1716758

881 views

Original Research

Published on 21 Jan 2026

Modeling and validating of oxygen transport in wave bioreactors: optimized experimental mass transfer method and novel Lattice-Boltzmann CFD approach

in Bioprocess Engineering

S. Piontek
J. Fitschen
C. Weiland
T. Habicher
M. Schlüter
T. Wucherpfennig

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1688774

809 views

Brief Research Report

Published on 16 Jan 2026

Integrated biosynthesis of the lignan (-)-pluviatolide in resting and growing E. coli cells

in Bioprocess Engineering

Ronja Knöfel
Jonas Barsig
Philipp A. Bechtold
Cigdem Günes
U. Joost Luelf
Vlada B. Urlacher

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2026.1716646

520 views

Original Research

Published on 15 Jan 2026

Effects of low-serum adaptation on growth and protein expression in stably lactoferrin-overexpressing mammary alveolar cells

in Bioprocess Engineering

Zhenxing Qiang
Xiaoqian Cai
Zhenzhen Zhang
Hui Wang
Qiuying Wang
Yajing Ji
Guangpeng Li
Guanghua Su
Lei Yang
Weicang Qiao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2026.1731548

421 views

Review

Published on 15 Jan 2026

Bioleaching as a biotechnological tool for metal recovery: from sewage to space mining

in Bioprocess Engineering

Archana Paimpillil Abraham
Simone Schopf

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1712157

848 views

Original Research

Published on 12 Jan 2026

Life on the rocks: unexpected enzyme activity of the extremophilic black fungus Knufia chersonesos

in Bioprocess Engineering

Sophia Mihalyi
Christian Zimmermann
Felice Quartinello
Donatella Tesei
Astrid R. Mach-Aigner
Georg Guebitz
Doris Ribitsch

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1720118

533 views

Original Research

Published on 12 Jan 2026

Scalable CAR-T production in a 2-litre perfusion stirred-tank bioreactor with automated harvesting and scale-down model characterisation

in Bioprocess Engineering

Pierre Springuel
Pedro Silva Couto
Dale J. Stibbs
Michal Szelwicki
Amanda Frangleton
Timo Schmidberger
Ajith George
Fern Slingsby
Nicola Bevan
Asma Ahmad

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1694134

2,406 views

Review

Published on 09 Jan 2026

Cis-abienol from tobacco trichomes to ambergris-like compounds: advances in biosynthesis, fermentation, and industrial applications

in Bioprocess Engineering

Wenting Wang
Xinlong Zhang
Wei Hu
Shen Huang
Robina Manzoor
Aamir Rasool

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1713206

704 views

Review

Published on 08 Jan 2026

Prospects and challenges regarding biosurfactants in advancing the petroleum industry

in Bioprocess Engineering

Ojasvini Ahluwalia
Niketan Patel
Nikhil Narmeta
Sandra Guzman Sanchez
Alexandre Soares Rosado

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1697361

1,179 views

Original Research

Accepted on 07 Jan 2026

Impact of keto leucine and isoleucine on CHO cell central carbon metabolism and performance in fed-batch and steady-state perfusion

in Bioprocess Engineering

Philipp Reifenberg
Daniel Benjamin
Maxime Le Mignon
Aline Zimmer

Frontiers in Bioengineering and Biotechnology

245 views

Original Research

Published on 07 Jan 2026

Growth of Hydrogenophaga pseudoflava on syngas: design of gas and liquid medium composition based on elemental yield coefficients

in Bioprocess Engineering

Florian Miserez
Sven Panke
Manfred Zinn

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1727931

842 views

Original Research

Published on 05 Jan 2026

Tuning lipid accumulation and fitness of motile algae via hydrodynamic cues

in Bioprocess Engineering

Narges Kakavand
Anupam Sengupta

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1722499

732 views

Original Research

Accepted on 19 Dec 2025

A novel PolyAr87-based cell transfection protocol for nanobody expression optimized via a targeted Design of Transfection approach

in Bioprocess Engineering

Lautaro Fidel Bracco
Giovanna L. Liguori
ANTONELLA LANATI
Juan Manuel Lázaro-Martínez
Mariela Bollini
Leonardo Poggio
Marina Bok
Lorena Itatí Ibañez
Viviana Parreño

Frontiers in Bioengineering and Biotechnology

294 views

Review

Published on 10 Dec 2025

Microalgae biotechnology and its role in sustainable and healthy food design

in Bioprocess Engineering

Adriane Terezinha Schneider
Richard Luan Silva Machado
Darissa Alves Dutra
Eduarda Funari Machado
Rosangela Rodrigues Dias
Mariany Costa Deprá
Leila Queiroz Zepka
Eduardo Jacob-Lopes

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1716473

1,551 views

Editorial

Published on 08 Dec 2025

Editorial: Biotechnologies to recover critical metals

in Bioprocess Engineering

Denys Kristalia Villa Gomez
Eric D. van Hullebusch
Aurora M. Pat Espadas
Iván Nancucheo

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1747699

1,458 views

Methods

Published on 03 Dec 2025

Updated transient gene expression protocol in Expi293F cells using PEI

in Bioprocess Engineering

Gustaf Hederoth
Andrés de la Rosa
Ana Godec
Ximena Aguilar
Antonino Napoleone
Alex Petrovic
Nicole G. Metzendorf
Greta Hultqvist

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1661193

1,807 views
1 citation

Review

Published on 26 Nov 2025

Bioleaching for critical metal recovery from bauxite residue- unlocking waste valorization

in Bioprocess Engineering

Fernanda Soto-Montandon
Rosemary Gillane
Esteban Marcellin
Susan T. L. Harrison
Denys Villa-Gomez

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1685819

3,044 views

Original Research

Published on 21 Nov 2025

Impact of multiplicity of infection and baculovirus co-infection ratio on recombinant adeno-associated virus vector production in insect cells

in Bioprocess Engineering

Mels Schrama
Linda A. de Jong
Stefan Kint
Pranav Puri
Femke Hoeksema
Gorben P. Pijlman
Monique M. van Oers
Rene H. Wijffels
Dirk E. Martens

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1679358

1,592 views

Original Research

Published on 19 Nov 2025

Extraction, purification and antioxidant activity of flavonoids from Potentilla anserina L

in Bioprocess Engineering

Dandan Gao
Xuankang Yang
Danqi Lu
Chenchen Wang
Jiwen Wang
Huaxin Li
Xingchen Guo

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1691606

1,170 views

Original Research

Published on 18 Nov 2025

Development of 10 L mass culture system of human induced pluripotent stem cells with intermittent agitation using plastic fluid

in Bioprocess Engineering

Tomohiro Tokura
Riku Yamamoto
Masahiro Kino-oka

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1664723

1,779 views

Original Research

Published on 07 Nov 2025

Municipal solid waste gasification for resilient energy systems: advancing sustainable crisis preparedness in the wake of COVID-19

in Bioprocess Engineering

Mohammad Rehan
Khurram Shahzad
Nadeem Ali
Abdul-Sattar Nizami

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1691738

1,782 views

Original Research

Published on 23 Oct 2025

Dual formation mechanisms of acidic variants in cysteine-engineered antibodies and strategies for their reduction

in Bioprocess Engineering

Shasha Zhou
Xinyu Cao
Xuefei Yin
Jiawen Xu
Kai Gao
Zhenshou Wang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1615263

2,087 views

1,011 articles

articles

Towards a cleaner CHO chassis: systematic knockout of host cell proteins for efficient biopharmaceutical manufacturing

Structural Improvement Towards the Efficiency of Biodigesters in the 21st Century: A Review of the Different Designs

Whole-genome DNA methylation analysis of Chinese hamster ovary cells undergoing media adaptation

Modeling and validating of oxygen transport in wave bioreactors: optimized experimental mass transfer method and novel Lattice-Boltzmann CFD approach

Integrated biosynthesis of the lignan (-)-pluviatolide in resting and growing E. coli cells

Effects of low-serum adaptation on growth and protein expression in stably lactoferrin-overexpressing mammary alveolar cells

Bioleaching as a biotechnological tool for metal recovery: from sewage to space mining

Life on the rocks: unexpected enzyme activity of the extremophilic black fungus Knufia chersonesos

Scalable CAR-T production in a 2-litre perfusion stirred-tank bioreactor with automated harvesting and scale-down model characterisation

Cis-abienol from tobacco trichomes to ambergris-like compounds: advances in biosynthesis, fermentation, and industrial applications

Prospects and challenges regarding biosurfactants in advancing the petroleum industry

Impact of keto leucine and isoleucine on CHO cell central carbon metabolism and performance in fed-batch and steady-state perfusion

Growth of Hydrogenophaga pseudoflava on syngas: design of gas and liquid medium composition based on elemental yield coefficients

Tuning lipid accumulation and fitness of motile algae via hydrodynamic cues

A novel PolyAr87-based cell transfection protocol for nanobody expression optimized via a targeted Design of Transfection approach

Microalgae biotechnology and its role in sustainable and healthy food design

Editorial: Biotechnologies to recover critical metals

Updated transient gene expression protocol in Expi293F cells using PEI

Bioleaching for critical metal recovery from bauxite residue- unlocking waste valorization

Impact of multiplicity of infection and baculovirus co-infection ratio on recombinant adeno-associated virus vector production in insect cells

Extraction, purification and antioxidant activity of flavonoids from Potentilla anserina L

Development of 10 L mass culture system of human induced pluripotent stem cells with intermittent agitation using plastic fluid

Municipal solid waste gasification for resilient energy systems: advancing sustainable crisis preparedness in the wake of COVID-19

Dual formation mechanisms of acidic variants in cysteine-engineered antibodies and strategies for their reduction