Frontiers in Bioengineering and Biotechnology

Original Research

Published on 04 Apr 2025

Autofluorescence lifetime imaging classifies human B and NK cell activation state

in Cell and Gene Therapy

Rebecca L. Schmitz
Jeremiah M. Riendeau
Kelsey E. Tweed
Peter Rehani
Kayvan Samimi
Dan L. Pham
Isabel Jones
Elizabeth M. Maly
Emmanuel Contreras Guzman
Matthew H. Forsberg

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1557021

3,366 views
1 citation

Review

Published on 03 Apr 2025

Extracellular vesicles: innovative cell-free solutions for wound repair

in Cell and Gene Therapy

Hanne Eerdekens
Elke Pirlet
Sarah Willems
Annelies Bronckaers
Paula M. Pincela Lins

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1571461

4,754 views
8 citations

Original Research

Published on 21 Mar 2025

Lymphoblastoid and Jurkat cell lines are useful surrogate in developing a CRISPR-Cas9 method to correct leukocyte adhesion deficiency genomic defect

in Cell and Gene Therapy

Ahmad R. Ramadan
Noureddine Ben Khalaf
Khaled Trabelsi
Halla Bakheit
Imen Ben-Mustapha
Mohamed-Ridha Barbouche
M-Dahmani Fathallah

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1548227

2,886 views
1 citation

Original Research

Published on 20 Mar 2025

Towards propagation of epidermal cells for wound repair: glass, as cell culture substrate, enhances proliferation and migration of human keratinocytes

in Cell and Gene Therapy

Hady Shahin
Ingrid Steinvall
Folke Sjöberg
Moustafa Elmasry
Ahmed El-Serafi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1547044

2,502 views
1 citation

Opinion

Published on 19 Mar 2025

CGT 4.0: a distant dream or inevitable future? Smart process automation is critical to make efficient scalability of CGT manufacturing a reality

in Cell and Gene Therapy

Aleksander Szarzynski
Oliver Spadiut
Matthias Reisbeck
Gerhard Jobst
Rachel L. Paterson
Anna Kamenskaya
Emilie Gateau
Hanna P. Lesch
Luc Henry
Bence Kozma

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1563878

7,898 views
2 citations

Original Research

Published on 05 Feb 2025

The landscape of cell regulatory and communication networks in the human dental follicle

in Cell and Gene Therapy

Jia-Ning Liu
Jiong-Yi Tian
Lu Liu
Yuan Cao
Xiao Lei
Xiao-Hui Zhang
Zi-Qi Zhang
Jun-Xi He
Chen-Xi Zheng
Chao Ma

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1535245

3,561 views
2 citations

Review

Published on 29 Jan 2025

Application of biomaterials in mesenchymal stem cell based endometrial reconstruction: current status and challenges

in Cell and Gene Therapy

Ling He
Qianrong Li

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1518398

5,158 views
9 citations

Original Research

Published on 23 Jan 2025

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

in Cell and Gene Therapy

Elin Arvidsson
Diana Duarte Lobo
Ermelinda Sabarese
Fabio Duarte
Rui Jorge Nobre
Luis Quintino
Cecilia Lundberg

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1336313

3,026 views
1 citation

Review

Published on 15 Jan 2025

Approaches and applications in transdermal and transpulmonary gene drug delivery

in Cell and Gene Therapy

Anni Zhang
Xuran Zhang
Jiahui Chen
Xianbao Shi
Xijuan Yu
Zhonggui He
Jin Sun
Mengchi Sun
Zhijun Liu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1519557

3,842 views
3 citations

Methods

Published on 20 Nov 2024

Device for automated aseptic sampling: Automated sampling solution for future cell and gene manufacturing

in Cell and Gene Therapy

Liu Dan
Wu Ying Ying
Akshaya V. Prabhu
Ahmad Amirul bin Abdul Rahim
Zach Lee Jia Sheng

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1452674

4,451 views
2 citations

Original Research

Published on 20 Nov 2024

Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy

in Cell and Gene Therapy

Andrew Brovin
Ekaterina Minskaia
Matvei Sabantsev
Sergey Chuvpilo
Alexander Karabelsky

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1488912

6,574 views
4 citations

Original Research

Published on 11 Nov 2024

Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids

in Cell and Gene Therapy

Haneen S. Alsehli
Errin Roy
Thomas Williams
Alicja Kuziola
Yunzhe Guo
Cecile A. Dreiss
Jeremy B.A. Green
Eileen Gentleman
Davide Danovi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1467412

3,382 views
1 citation

Original Research

Published on 01 Oct 2024

An improved high-resolution method for quantitative separation of empty and filled AAV8 capsids by strong anion exchange HPLC

in Cell and Gene Therapy

Samantha Schrecke
Kevin McManus
Cassandra Moshfegh
Jessica Stone
Thuy-Uyen Nguyen
Gustavo Rivas
Ismaeel Muhamed
Daniel A. J. Mitchell

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1436857

5,086 views
4 citations

Original Research

Published on 20 Aug 2024

Assessing a single-cell multi-omic analytic platform to characterize ex vivo-engineered T-cell therapy products

in Cell and Gene Therapy

Maryam Moshref
Jerry Hung-Hao Lo
Andrew McKay
Julien Camperi
Joseph Schroer
Norikiyo Ueno
Shu Wang
Saurabh Gulati
Somayeh Tarighat
Steffen Durinck

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1417070

10,774 views
3 citations

Review

Published on 29 Jul 2024

The application potential of iMSCs and iMSC-EVs in diseases

in Cell and Gene Therapy

Xin Zhou
Jinyu Liu
Feifeng Wu
Jueyi Mao
Yang Wang
Junquan Zhu
Kimsor Hong
Haotian Xie
Binbin Li
Xinying Qiu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1434465

5,803 views
9 citations

Systematic Review

Published on 22 Jul 2024

Effectiveness and mechanisms of mesenchymal stem cell therapy in preclinical animal models of hepatic fibrosis: a systematic review and meta-analysis

in Cell and Gene Therapy

Xuesong Wang
Yue Wang
Wenming Lu
Jiayang Qu
Yang Zhang
Junsong Ye

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1424253

4,701 views
8 citations

Original Research

Published on 10 Jul 2024

Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

in Cell and Gene Therapy

Eivina Radzevičiūtė-Valčiukė
Jovita Gečaitė
Austėja Balevičiūtė
Anna Szewczyk
Augustinas Želvys
Barbora Lekešytė
Veronika Malyško-Ptašinskė
Eglė Mickevičiūtė
Paulina Malakauskaitė
Julita Kulbacka

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1430637

5,461 views
4 citations

Original Research

Published on 05 Jul 2024

11β hydroxysteroid dehydrogenase type 1 transgenic mesenchymal stem cells attenuate inflammation in models of sepsis

in Cell and Gene Therapy

Rahul Y. Mahida
Zhengqiang Yuan
Krishna K. Kolluri
Aaron Scott
Dhruv Parekh
Rowan S. Hardy
Michael A. Matthay
Gavin D. Perkins
Sam M. Janes
David R. Thickett

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1422761

5,462 views

Original Research

Published on 18 Jun 2024

Use of CRISPRoff and synthetic Notch to modulate and relay endogenous gene expression programs in engineered cells

in Cell and Gene Therapy

Shuqun Shi
Catherine A. Hamann
Joanne C. Lee
Jonathan M. Brunger

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1346810

4,502 views
3 citations

Original Research

Published on 31 May 2024

Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells

in Cell and Gene Therapy

Dan Cappabianca
Jingling Li
Yueting Zheng
Cac Tran
Kassandra Kasparek
Pedro Mendez
Ricky Thu
Travis Maures
Christian M. Capitini
Robert Deans

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1379900

8,474 views
6 citations

Review

Published on 24 May 2024

Designing cytochrome P450 enzymes for use in cancer gene therapy

in Cell and Gene Therapy

Saskya E. Carrera-Pacheco
Alexander Mueller
Juan A. Puente-Pineda
Johana Zúñiga-Miranda
Linda P. Guamán

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1405466

14,703 views
10 citations

Review

Published on 23 May 2024

Advances in ex vivo expansion of hematopoietic stem and progenitor cells for clinical applications

in Cell and Gene Therapy

André Branco
Janakiram Rayabaram
Cláudia C. Miranda
Ana Fernandes-Platzgummer
Tiago G. Fernandes
Suchitra Sajja
Cláudia L. da Silva
Mohan C. Vemuri

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1380950

10,534 views
9 citations

Systematic Review

Published on 06 May 2024

Evaluating the efficacy of mesenchymal stem cells for diabetic neuropathy: A systematic review and meta-analysis of preclinical studies

in Cell and Gene Therapy

Yu Li
Guangren Yue
Shuying Yu
Xinhao Cheng
Yilin Cao
Ximei Wang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1349050

4,396 views
7 citations

Original Research

Published on 26 Apr 2024

Cell-free fat extract regulates oxidative stress and alleviates Th2-mediated inflammation in atopic dermatitis

in Cell and Gene Therapy

Zexin Fu
Qinhao Gu
Lu Wang
Lulu Chen
Liuyi Zhou
Qiang Jin
Ting Li
Ye Zhao
Sufan Wu
Xuejiao Luo

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1373419

4,287 views
6 citations

323 articles

articles

Autofluorescence lifetime imaging classifies human B and NK cell activation state

Extracellular vesicles: innovative cell-free solutions for wound repair

Lymphoblastoid and Jurkat cell lines are useful surrogate in developing a CRISPR-Cas9 method to correct leukocyte adhesion deficiency genomic defect

Towards propagation of epidermal cells for wound repair: glass, as cell culture substrate, enhances proliferation and migration of human keratinocytes

CGT 4.0: a distant dream or inevitable future? Smart process automation is critical to make efficient scalability of CGT manufacturing a reality

The landscape of cell regulatory and communication networks in the human dental follicle

Application of biomaterials in mesenchymal stem cell based endometrial reconstruction: current status and challenges

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

Approaches and applications in transdermal and transpulmonary gene drug delivery

Device for automated aseptic sampling: Automated sampling solution for future cell and gene manufacturing

Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy

Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids

An improved high-resolution method for quantitative separation of empty and filled AAV8 capsids by strong anion exchange HPLC

Assessing a single-cell multi-omic analytic platform to characterize ex vivo-engineered T-cell therapy products

The application potential of iMSCs and iMSC-EVs in diseases

Effectiveness and mechanisms of mesenchymal stem cell therapy in preclinical animal models of hepatic fibrosis: a systematic review and meta-analysis

Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

11β hydroxysteroid dehydrogenase type 1 transgenic mesenchymal stem cells attenuate inflammation in models of sepsis

Use of CRISPRoff and synthetic Notch to modulate and relay endogenous gene expression programs in engineered cells

Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells

Designing cytochrome P450 enzymes for use in cancer gene therapy

Advances in ex vivo expansion of hematopoietic stem and progenitor cells for clinical applications

Evaluating the efficacy of mesenchymal stem cells for diabetic neuropathy: A systematic review and meta-analysis of preclinical studies

Cell-free fat extract regulates oxidative stress and alleviates Th2-mediated inflammation in atopic dermatitis