Novel Potential Biomarkers for Predicting Childhood Caries via Metagenomic Analysis

Zhang Hui¹Zheng Xiao²Huang Yong mao¹Yuanqiang ZOU³Zhang Tao³Maria Alice Repo¹Yin Mei xiang⁴You Yang^5*Jie Zhu ye^3*Xu Wen an^1*

• ¹Southern Medical University, Guangzhou, China
• ²Fudan University, Shanghai, Shanghai Municipality, China
• ³Beijing Genomics Institute (BGI), Shenzhen, China
• ⁴Shenzhen Samii International Medical Center, Shenzhen, China
• ⁵Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China

Background: Dental caries is a prevalent global health issue, particularly among children, with significant oral and overall health implications. The oral microbiome is considered a critical factor in caries development, with various microbial species implicated in the disease process.Objectives: This study aims to explore the changes and interactions of oral microbiota in childhood caries using metagenomic analysis, and identify potential biomarkers for early caries detection and treatment.Methods: Saliva samples were collected from 241 children aged 6 to 9 years, categorized into caries free (CF), low-moderate caries (CL), and caries severe (CS) groups. Metagenomic sequencing was performed to analyze the oral microbiome, followed by a series of statistical and functional analyses to characterize microbial diversity and function.The study revealed significant differences in the microbial community composition among the groups, with the CS group exhibiting higher alpha and beta diversity than that of the CF group.Numerous unclassified microorganisms, such as Campylobacter SGB19347 and Catonella SGB4501, are intimately linked to dental caries and display intricate interaction networks, suggesting the potential formation of a distinct ecological network. In functional assessment, we identified a possible link between pectin and caries, suggesting that microorganisms that produce pectinase enzymes might play a role in the advancement of severe dental caries. Additionally, we identified 16 species as the best marker for severe dental caries, achieving an impressive AUC of 0.91.The role of microbiota in dental caries is multifaceted, involving a complex interplay of microbial species and functions. Our findings enhance the understanding of the microbial basis of dental caries and offer potential diagnostic and therapeutic targets. The predictive capacity of the identified biomarkers warrants further investigation for early caries detection and intervention.The identification of novel biomarkers through metagenomic analysis enables early detection and targeted intervention for childhood caries, potentially transforming children dental care and significantly improving long-term oral health outcomes.