ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Intestinal Microbiome
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1535477
This article is part of the Research TopicNew Insights into Gut Microbiota in Colorectal CancerView all 4 articles
Bacterial Profiling of Colorectal Cancer Biopsies: A Culture-Based Study in Indian Patients
Provisionally accepted
- 1Gene regulation laboratory, National Institute of Immunology (NII), New Delhi, India
- 2Department of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi, National Capital Territory of Delhi, India
- 3Department of Biotech. School of Life Sciences, Mahatma Gandhi Central University, Motihari, Bihar, India
- 4National Institute of Immunology (NII), New Delhi, India
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Emerging research has highlighted the significant role of microorganisms in the initiation and progression of colorectal cancer (CRC). However, further investigation is required to elucidate the precise mechanisms by which the microbial community or specific bacteria contribute to carcinogenesis. The present work deals with the isolation and identification of bacteria from 9 colorectal cancer biopsy samples and 9 adjacent normal biopsy samples. Different media, such as Brain Heart Infusion (BHI), Anaerobic Basal Agar (ABA), Trypticase Soy Agar (TSA), and culture conditions have been manipulated to maximize the isolation of bacteria residing in biopsy samples.A total of 75 bacteria were isolated from the tumor and adjacent site. Enterococcus, Escherichia, Klebsiella, Shigella, Citrobacter, Morganella, and Veillonella have been found to be enriched in most of the tumor biopsies, while biopsies collected from adjacent tissues had Escherichia, Shigella, Enterococcus, and Streptococcus bacteria. A culture-based approach to assessing bacterial diversity offers advantages, enabling the study of individual bacteria to elucidate mechanisms of intestinal carcinogenesis. This culture-based approach may provide novel insights into pathology and potentially lead to new therapeutic modalities targeting the specific bacteria implicated in the inflammation and carcinogenesis of colorectal cancer.
Keywords: colorectal cancer, Gut Microbiota, culturomics, bacterial diversity, Carcinogenesis
Received: 27 Nov 2024; Accepted: 06 Jun 2025.
Copyright: © 2025 Kushwaha, Chaudhary, Singh, Makharia and Kumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Anil Kumar, National Institute of Immunology (NII), New Delhi, India
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