Skin Microbiome-Biophysical Association: A First Integrative Approach to Classifying Korean Skin Types and Aging Groups

Seyoung Mun¹Hyungwoo Jo²Youngmock Heo²Chaeyun Baek²Hye-Been Kim²Haeun Lee²KyeongEui Yun³Jinuk Jeong¹Wooseok Lee¹Dasom Jeon⁴So Min Kang⁴Seunghyun Kang²Young-Bong Choi¹Sangjin Han³Gabriel Kim³Kung Ahn³Dong Hun Lee⁵Yong Ju Ahn³Dong-Geol Lee²Kyudong Han^6*

• ¹Dankook University, Cheonan, Republic of Korea
• ²COSMAX BTI, Seongnam-si, Republic of Korea
• ³HuNBiome, Seoul, Republic of Korea
• ⁴Korea Biomedical Research Institute, Seongnam-si, Republic of Korea
• ⁵Seoul National University, Seoul, Republic of Korea
• ⁶Dankook University, Yongin, Republic of Korea

The field of human microbiome research is rapidly expanding beyond the gut and into the facial skin care industry. However, there is still no established criterion to define the objective relationship between the microbiome and clinical trials for developing personalized skin solutions that consider individual diversity.Objectives: In this study, we conducted an integrated analysis of skin measurements, clinical Baumann skin type indicator (BSTI) surveys, and the skin microbiome of 950 Korean subjects to examine the ideal skin microbiome-biophysical associations.Methods: By utilizing four skin biophysical parameters, we identified four distinct Korean Skin Cutotypes (KSCs) and categorized the subjects into three aging groups: the Young (under 34 years old), the Aging I group (35-50), and the Old group (over 51). To unravel the intricate connection between the skin's microbiome and KSC types, we conducted DivCom clustering analysis.Results: This endeavor successfully classified 726 out of 740 female skin microbiomes into three subclusters: DC1-sub1, DC1-sub2, and DC2 with 15 core genera. To further amplify our findings, we harnessed the potent capabilities of the CatBoost boosting algorithm and achieved a reliable framework for predicting skin types based on microbial composition with an impressive average accuracy of 0.96 AUC value. Our study conclusively demonstrated that these 15 core genera could serve as objective indicators, differentiating the microbial composition among the aging groups.In conclusion, this study sheds light on the complex relationship between the skin microbiome and biophysical properties, and the findings provide a promising approach to advance the field of skincare, cosmetics, and broader microbial research.