ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Clinical Microbiology
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1566603
This article is part of the Research TopicAdvances in the Diagnosis and Management of Infectious DiseasesView all 18 articles
A Combination of Recombinase Polymerase Amplification with CRISPR Technology Rapidly Detects Goose Parvovirus with High Accuracy and Sensitivity
Provisionally accepted
- Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, China
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Goose parvovirus (GPV) poses a significant threat to the waterfowl industry, highlighting the critical need for prompt and accurate on-site diagnostics for effective disease control. To address this challenge, we developed a novel assay that seamlessly integrates recombinase polymerase amplification (RPA) with CRISPR/Cas12a technology for rapid GPV nucleic acids detection. This innovative method achieves a detection limit of 10 copies/μL targeting the VP3 gene within one hour, ensuring both sensitivity and rapid turnaround time. The assay demonstrated exceptional specificity, showing no cross-reactivity with other waterfowl viruses, and exhibited robust reproducibility with intra-and inter-assay coefficients of variation consistently below 5.0%. Clinical validation using 42 field samples confirmed a diagnostic sensitivity of 100% and 95.5% specificity, outperforming real-time quantitative PCR (qPCR) in both metrics. Furthermore, the assay supports flexible readouts via portable blue-light transilluminators, enabling visual on-site interpretation. Our RPA-CRISPR/Cas12a assay represents a breakthrough in GPV diagnostics, offering a robust, user-friendly, and highly reliable approach for resource-limited settings.
Keywords: goose parvovirus, CRISPR/Cas12a, Recombinase polymerase amplification, Nucleic Acid, Portable
Received: 25 Jan 2025; Accepted: 31 May 2025.
Copyright: © 2025 Chen, Zhang, Lin, Wang, Huang, Chen and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xiuqin Chen, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, China
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