ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Antibiotic Resistance and New Antimicrobial drugs
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1572799
This article is part of the Research TopicMultidrug Resistant Gram-negative Bacteria in Fragile HostsView all 7 articles
Prevention of Donation-Related Infections: Investigating the Use of Antibiotics in the Decontamination of Preservation Fluid for Organ Transplantation
Provisionally accepted
- Changhai Hospital, Second Military Medical University, Shanghai, China
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Background: Donation-related infections (DRIs), particularly those caused by carbapenem-resistant gram-negative bacteria (CRGNB), can have disastrous consequences because of their extensive drug resistance. Contamination during graft acquisition and transport can lead to DRIs, and the use of antibiotics in preservation fluid (PF) before organ transplantation can reduce the incidence of DRIs. This study was to determine and compare the effectiveness of different PF decontamination regimens to prevent CRGNB related DRIs.Methods: Twelve CRGNB strains were chosen to be the targets of decontamination, and a drug concentration gradient was established for each test drug based on the previous clinical research. In addition the standard decontamination procedures were performed to evaluate the antimicrobial effectiveness of polymyxin B (PB), colistin sulfate (CS), colistimethate sodium (CMS) and amikacin (AK) in the 0~4℃ PF, and to explore the antimicrobial effects of CMS after different preprocessing methods.Results: PB and CS exhibited significantly better antimicrobial effectiveness against CRGNB than AK and CMS in the 0~4℃ PF, and the antimicrobial effects on CRGNB increased with the increasing concentration of drugs. Notably, CMS after pretreatment (CMS-AP), its antibacterial was significantly enhanced at 4°C.Conclusions: The PF decontamination is important in preventing the DRIs caused by CRGNB, and the the decontamination regimens based on PB or CS were confirmed effective. Notably, CMS could even achieve a better decontamination effect than PB after a simple and fast pretreatment.
Keywords: donation-related infection, CRGNB, Decontamination, polymyxin, transplant
Received: 07 Feb 2025; Accepted: 04 Jul 2025.
Copyright: © 2025 Duan, Yu, Zhang, Li, Chen, Li, Wan, Wu, Li and Sui. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Mingxing Sui, Changhai Hospital, Second Military Medical University, Shanghai, China
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