ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Extra-intestinal Microbiome
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1574511
This article is part of the Research TopicAdvancements in Sepsis Diagnosis Utilizing Next-Generation Sequencing Approaches for Personalized MedicineView all 9 articles
The Intratumoral Microbiota Heterogenicity is Related to the Prognosis and tumorigenesis of Cervical Cancer
Provisionally accepted
- 1Department of Gynecology, Shanghai East Hospital, School of Medicine, Tongji University,, Shanghai, China
- 2Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,, Wuhan, Hubei Province, China
- 3Department of Obstetrics and Gynaecology, Queen Mary Hospital, Hong Kong SAR, Hong Kong, SAR China
- 4Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Background：The intratumoral microbe-host interaction plays crucial role in the development of cancer. The microbiome can influence cancer development by modulating inflammation, immune responses and metabolic pathways. Therefore, we aim to delineate the landscape and role of intratumoral microbiota in cervical cancer (CC).Methods：The presence of bacterial community in CC tissues was confirmed by fluorescence in situ hybridization (FISH). Then 16s rRNA and RNA-Seq were used to characterize the composition of intratumoral microbiota. Combined with cervical squamous cell carcinoma (CESC) data from the Tumor Cancer Genome Atlas (TCGA), the clinical signatures of intratumoral microbiota and DEGs were further analyzed. Finally, the effect of the up-regulated Fibrinogen beta chain (FGB) expressed fragment peptide on the biological behavior of cancer was verified in vitro.Results：We found the composition heterogeneity of bacteria in CC tumors. Pseudomonas was most highly enriched in CC tissues and grouped according to the relative abundance level. The clinical characteristics of patients with relatively high abundance of Pseudomonas had the higher levels of fibrinogen and lower levels of white blood cell (WBC) and albumin (ALB) expression.Combining transcriptome data from the two our collective CC and TCGA-CESC cohorts, we found that Pseudomonas abundance was significantly associated with fibrinogen beta peptide expression and worse overall survival in CC patients. In vitro experiment revealed that Pseudomonas could promote the proliferation and migration of cervical cancer cells through overexpression of FGB.Conclusions：We characterized the composition of the intratumoral microbiota in CC tissues and identified the most significantly differentially abundant bacteria between cancerous and non-cancerous tissues. Our findings provide novel insights into the relationship between intratumoral Pseudomonas and the tumorigenesis of CC. A deeper understanding of the tumor microenvironment and its associated microbiota may reveal new potential therapeutic targets and improve clinical outcomes.
Keywords: intratumoral microbiota, cervical cancer, Pseudomonas, Fibrinogen, prognosis
Received: 11 Feb 2025; Accepted: 14 Apr 2025.
Copyright: © 2025 Guo, Xiao, Wang, Wang, Cao, Tse, Han, Li and Zhi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zhiqiang Han, Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei Province, China
Fang Li, Department of Gynecology, Shanghai East Hospital, School of Medicine, Tongji University,, Shanghai, China
Yong Zhi, Department of Gynecology, Shanghai East Hospital, School of Medicine, Tongji University,, Shanghai, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.