ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Clinical Microbiology
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1579074
This article is part of the Research TopicNovel Diagnostics to Aid in Early Detection of Antimicrobial Resistance (AMR)View all articles
EVALUATION OF MOLECULAR MOUSE SEPSIS PANEL: NEW PORTABLE AND RAPID TESTS FOR MICROORGANISM DETECTION IN SUSPECTED BLOOD STREAM INFECTION
Provisionally accepted
Claudia Colosimo1,2*
Maria Sofia Montanari1,2*Anna Marzucco1,2
Giulia Gatti1,2
Lucrezia Puccini1Laura Grumiro1,2
Laura Dionisi1,2Massimiliano Guerra1,2
Martina Brandolini1,2
Ludovica Ingletto1,2Giorgio Dirani1
Silvia Zannoli1
Alessandra Scagliarini2
Alessandra Mistral De Pascali1,2
Vittorio Sambri1,2
Monica Cricca1,2- 1Operative Unit of Microbiology, The Great Romagna Hub Laboratory, Pievesestina, Italy
- 2University of Bologna, Bologna, Italy
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Bloodstream infections (BSIs) represent a significant public health concern, characterized by the presence of pathogens in the bloodstream, leading to serious conditions. Between 30 and 40% of BSIs evolve in sepsis, characterized by a life-threatening organ dysfunction and accompanied by a strong or dysregulated systemic immune or inflammatory response. A timely and accurate diagnosis of BSIs is crucial for ensuring effective management and treatment of critically ill patients. The diagnosis of BSIs can be initiated either from positive blood culture (BC) or directly from blood specimens using advanced diagnostic methods, these approaches are increasingly integrated into traditional diagnostic workflows. In the context of emerging molecular methods, Alifax introduced the Molecular Mouse (MM) Sepsis Panel in 2022. This study aims to evaluate the performance of MM cartridges in detecting Gram-positive bacteria and Gram-negative bacteria, as well as their associated resistance genes, using clinical leftover positive BCs and contrived samples for low-prevalence targets. Positive BCs were collected at the Unit of Microbiology, The Great Romagna Hub Laboratory, Pievesestina, FC, Italy. Overall, MM Sepsis Panel showed excellent sensitivity (98.62%) and specificity (99.81%), confirming the system’s reliability and accuracy. The high diagnostic accuracy demonstrated by these tests supports their potential application as rapid diagnostic tools for blood cultures, facilitating prompt identification of pathogens and antimicrobial resistance determinants in clinical microbiology settings.
Keywords: Molecular mouse, Blood Stream infection (BSI), Molecular diagnoses, rapid test, Sepsis
Received: 18 Feb 2025; Accepted: 04 Jul 2025.
Copyright: © 2025 Colosimo, Montanari, Marzucco, Gatti, Puccini, Grumiro, Dionisi, Guerra, Brandolini, Ingletto, Dirani, Zannoli, Scagliarini, De Pascali, Sambri and Cricca. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Claudia Colosimo, Operative Unit of Microbiology, The Great Romagna Hub Laboratory, Pievesestina, Italy
Maria Sofia Montanari, Operative Unit of Microbiology, The Great Romagna Hub Laboratory, Pievesestina, Italy
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.