REVIEW article
Front. Cell. Infect. Microbiol.
Sec. Microbes and Innate Immunity
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1580337
This article is part of the Research TopicRNA Regulation Mechanisms in Microbial-Host InteractionsView all 3 articles
Emerging RNA-centric technologies to probe RNA-protein interactions: Importance in decoding the life cycle of positive sense single strand RNA viruses and antiviral discovery
Provisionally accepted
- Translational Health Science and Technology Institute (THSTI), Faridabad, India
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Positive sense single strand RNA (+ssRNA) viruses are one of the evolutionary successful organisms and many of them pose a significant threat to human health. Diseases caused by +ssRNA viruses such as COVID-19, Flu and acute viral hepatitis are major public health concern worldwide. Therefore, a lot of research is focused at decoding the life cycle of +ssRNA viruses and develop specific antiviral therapeutics against them. Interaction of the viral RNA with virus-encoded proteins and host proteins drives the lifecycle and pathogenesis of +ssRNA viruses. Recent developments in computational and high-throughput omics-based experimental technologies offer the sensitivity and specificity for molecular characterization of these RNA-protein complexes. These are promising tools to revolutionize the field of +ssRNA virus research and pave the way for antiviral discovery. This review summarises the current scientific resources available to characterize the RNA-protein interactome of +ssRNA viruses and provides an overview of the drug discovery pipeline for developing antivirals against pathogenic +ssRNA viruses.
Keywords: RNA protein interactions, RNA binding protein, Positive strand RNA virus, Rapid assay, RAP-MS
Received: 20 Feb 2025; Accepted: 30 Apr 2025.
Copyright: © 2025 Ghosh, Kumar, Verma, Ansari, Chatterjee and Surjit. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Milan Surjit, Translational Health Science and Technology Institute (THSTI), Faridabad, India
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