- 1McFarland Consulting, Seattle, WA, United States
- 2Public Health Reserves Corps, Seattle, WA, United States
- 3Department of Gastroenterology, First Affiliated Hospital of Dalian Medical University, Dalian, China
Background: Pediatric acute gastroenteritis (PAGE) is a common cause of morbidity and mortality, especially in children under five years old. Therapeutic strategies including probiotics have been investigated, but trials from non-English speaking countries may not be easily accessible.
Aim: To determine the efficacy of Saccharomyces boulardii compared to controls for treating PAGE in children receiving standard rehydration therapy in trials conducted in China.
Methods: Systematic review and meta-analysis using literature search with Google Scholar, PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure and China Biology Medicine disc (from inception to June 30, 2024) of randomized, controlled trials comparing S. boulardii CNCM I-745 to controls for the treatment of PAGE in children conducted in China. Independent data extraction by two reviewers. Standard meta-analysis methods were applied and random-effect or fixed-effects models were used depending upon the degree of heterogeneity using standardized mean differences for continuous data and relative risk estimates for dichotomous outcomes. The risk of bias for each study was determined and heterogeneity was measured by I2.
Results: Of 851 articles screened, 10 RCTs (1125 participants) met the inclusion criteria, and none were found in non-Chinese databases. S. boulardii CNCM I-745 was found to significantly reduce the duration of PAGE (SMD=-1.63 days, 95% CI -2.08, -1.18), improve the total effectiveness rating (RR=1.22, 95% CI 1.16, 1.28) and significantly more participants were cured (RR=1.47, 95% CI 1.30, 1.67). The finding that S. boulardii significantly reduced the levels of two pro-inflammatory cytokines (TNF-α and IL8) has not been reported in previous meta-analyses of PAGE.
Conclusion: S. boulardii CNCM I-745 is an effective treatment for PAGE and was well tolerated in trials done in China.
Systematic review registration: www.crd.york.ac.uk/PRSPERO, identifier CRD 42024567537.
Introduction
Pediatric acute gastroenteritis (PAGE) is the third leading cause of morbidity, with more than 1.7 billion cases/year and mortality (nearly 500,000 million deaths/year) worldwide in children under five years old (Aghsaeifard et al., 2022; World Health Organization, 2024). Consequences of PAGE include increased risk of hospitalization and emergency room visits and an increased risk of dehydration and mortality (Zhang et al., 2016; Aghsaeifard et al., 2022). The incidence of PAGE varies globally by country, but also within provinces in China (Wang et al., 2021; World Health Organization, 2024).
Current guidelines for PAGE treatments include oral rehydration therapy (ORT), continued oral feeding and anti-infective medications for viral and bacterial etiologies (Chen et al., 2018). Challenges for these recommendations include the low frequency of ORT use in underdeveloped countries due to poor education for its use, inadequate water quality and limited access to medical resources (Troeger et al., 2020; Aghsaeifard et al., 2022). In addition, to shorten the duration of diarrhea and reduce its severity, several add-on therapies have been recommended, notably racecadotril, diosmectite, and certain probiotics including Saccharomyces boulardii CNCM I-745 and Lactobacillus rhamnosus GG (Guarino et al., 2018; Szajewska et al., 2023). However, a broad consensus is lacking for which probiotics are more effective due to high study heterogeneity when different probiotic types are pooled in meta-analyses (McFarland et al., 2018; Depoorter and Vandenplas, 2021).
Saccharomyces boulardii CNCM I-745 is a widely available probiotic yeast effective for a wide variety of intestinal diseases, including PAGE (McFarland, 2010; Szajewska et al., 2020). The exact mechanisms by which S boulardii exerts its actions are multifactorial and include the interference with pathogen attachment, restoration of disrupted intestinal microflora, inactivation of toxins (including Vibrio cholera, ETEC, Clostridioides difficile, etc.), antisecretory effects and immunomodulatory effects, both within the lumen and systemically (Stier and Bischoff, 2016; Plaza-Diaz et al., 2019). Many relevant clinical trials have been published in China, none of which have been included in published systematic reviews and meta-analyses published in the English literature.
The aim for this meta-analysis is to systematically review evidence on the effects of S boulardii CNCM I-745 compared with controls when children are treated with standard rehydration therapies for acute gastroenteritis in trials conducted in China.
Materials and methods
Our meta-analysis followed the 2020 Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines (see Supplementary Table S1) and recent recommendations to improve the reporting of probiotic meta-analyses (Page et al., 2021; McFarland et al., 2023). The protocol was prospectively registered with the International Prospective Register of Systematic Reviews (#CRD42024567537) on July 10, 2024 and is available at www.crd.york.ac.uk/PROSPERO/. The protocol was revised October 30, 2024 (more rigorous inclusion and exclusion criteria).
Search strategy
Open access literature databases [PubMed, Google Scholar, Embase, Cochrane Library, China National Knowledge Infrastructure (CNKI) and China Biology Medicine disc (CBMdisc)] were searched from inception to June 30, 2024, to identify randomized controlled trials (RCT) for the treatment of PAGE with Saccharomyces boulardii CNCM I-745 in China. Our search strategies are given in Supplementary Table S2. No language restrictions were imposed and publications in Chinese were translated into English. Secondary searches of grey literature included reference lists, authors, reviews, meeting abstracts websites and clinicaltrials.gov for unpublished trials.
Study screening
Inclusion criteria included: RCT with prospective parallel groups, randomized to either S. boulardii CNCM I-745 or control (open or placebo), children (≤18 years old) with acute diarrhea (<15 days) and living in China. Etiologies of acute diarrhea may include viral, bacterial, or unspecified. All participants were allowed conventional therapies (oral or IV rehydration therapy, antiviral medication or antibiotics as needed, diet changes, or correction of acid/base electrolytes). The intervention was a yeast (Saccharomyces boulardii CNCM I-745, “Yihuo”, Biocodex, France) given orally. Randomization of the study groups was required to be clearly stated in paper (not just ‘divided into two groups’ or not specified).
Exclusion criteria included: adult patients (>18 years old), studies not done in China, non-human studies, case reports or case series, early phase 1 (safety) or phase 2 (mechanism of action, dose ranging, formulation, kinetics) studies, retrospective case-control studies, no control group, intervention not well-described, no relevant outcomes provided, not a comparison of interest, reviews, meta-analysis, and duplicate reports or did not contain original quantitative data. Trials that gave additional treatments, including specific antibiotics not directed for bacterial diarrhea, zinc, montmorillonite, racecadotril, fructose or aluminum diphosphate, other probiotics, or Chinese medicines were excluded. Other types of diarrhea (AAD, IBS, diarrhea secondary to pneumonia, allergic diarrhea or lientery diarrhea) and trials comparing S. boulardii to another type of probiotic without a non-probiotic control group were excluded.
Study selection and data extraction
Two reviewers (LVM and TL) independently screened titles and abstracts of studies identified by the search strategies. Full articles were translated and screened with inclusion and exclusion criteria. Data from eligible articles were extracted and reviewed independently by two reviewers using a pre-designed data extraction form (see Supplementary Form S1, DEF) following guidelines for clinical trials and standard methods for systematic reviews and meta-analysis (Moher et al., 2010; Page et al., 2021; McFarland et al., 2023). Any disagreements were discussed with the reviewers until resolved. The data extracted included PICO data (1): Population (pediatric, age range, country) (2), Intervention (type of S. boulardii or controls used, daily doses, formulation, duration and follow-up times) (3), Comparisons (type of control group either placebo or open (4), Outcomes, including improvement in PAGE symptoms (duration of diarrhea, effectiveness rating, cure rate, stool frequency/day by end of study), time to resolution of vomiting or fever, length of hospitalizations, safety measures and changes in immune markers.
To assess sources of heterogeneity and their influence on efficacy, data on potential confounding factors were collected: study design (double blinded or open), risk of bias, study quality, age, setting (inpatient or outpatient) and etiology of diarrhea. For data not reported in the published article, we attempted to contact the author or co-authors to obtain the missing data.
Study quality
Quality and risk of bias were scored independently by both co-authors using standard methods (Sterne et al., 2001). The risk of bias (RoB) assessed with the RoB 2.0 tool and was graded (low, some concerns or high) for each of five types of bias (randomization process, deviations from intended interventions, missing outcome data, outcome measures, selection of reported result (Sterne et al., 2001). Study quality was also assessed for the 24 recommended items for clinical trials and graded as: high quality (≥75%), moderate quality (12–17 items present, 50-74%), and low quality (<12 items present, <50%). A summary table of risk of bias was generated and the effect of study quality was assessed in trials (McGuinness, 2024). GRADE analysis for selected outcomes was also conducted.
Primary outcome: resolution of diarrhea
Resolution of diarrhea was measured with four different outcome measures (1): Duration of diarrhea (defined as days of diarrhea from enrollment to the last day of reported diarrheal symptoms) (Aghsaeifard et al., 2022); Effectiveness rating for the improvement of diarrhea symptoms (defined into three categories: ‘markedly effective or cured’, resolution of diarrhea symptoms by the end of the intervention, ‘valid or improved’, moderate improvement in symptoms; and ‘not valid or no effect’, diarrhea symptoms persist at end of intervention, with Total Effectiveness Rating including ‘markedly effective (cured)’ plus ‘valid (improved)’ categories (3); Cured by end of treatment (defined as no diarrhea noted by end of treatment); and (4) Stool frequency per day at end of study treatment. Clinical data was typically recorded by staff if children were hospitalized.
Secondary outcomes
Four secondary outcomes were analyzed: (1) Frequency of adverse events (safety) during the study; (2) Changes in immune markers (cytokines) from baseline to study end; (3) Reduction in length of stay (if inpatients); (4) Resolution of upper gastrointestinal symptoms (days until vomiting, nausea or abdominal pain cessation). Levels of cytokines were quantified using standard laboratory assays at enrollment and end of intervention.
Sub-group analyses
Where sufficient data was reported, the efficacy of S. boulardii was analyzed by factors that may affect the degree of efficacy including etiology of diarrhea, duration of study intervention, daily dose of S. boulardii, risk of bias, study quality, study design (open or placebo controls) and age group.
Data analysis
Inclusion of studies in meta-analysis required >2 random controlled trials (RCT) using a common outcome measure comparing S. boulardii to a non-probiotic control. Statistical analysis and generation of forest plots of pooled summary estimates were performed using Stata software version 16 (Stata Corporation, College Station, Texas) with meta-analysis modules (Palmer and Sterne, 2016). Dichotomous outcomes were assessed using relative risks (RR) and 95% confidence intervals (CI) and continuous outcomes were assessed using standardized mean difference (SMD) and 95% confidence intervals (CI) using standard methods (Higgins et al., 2011). The significance level was set at p-value <0.05. Heterogeneity across trials was evaluated using the I2 statistic, 0% indicating none and >50% indicating a high degree of heterogeneity across the trials (Palmer and Sterne, 2016). Bayesian random effects models were used for the meta-analyses when high heterogeneity was found (I2>50%), otherwise fixed-effects models were used (Borenstein et al., 2010). Publication bias was assessed using funnel plots and the Egger’s test (Palmer and Sterne, 2016). Subgroup analyses were used to explore sources of heterogeneity and assessed with the Cochrane Q test (Higgins et al., 2011). Sequential sensitivity analysis was done to explore the extent outcomes were dependent upon a particular trial. Trials with missing data for an outcome measure were excluded from the models and no imputation methods were used for missing data.
Results
Literature search
The literature search found 851 abstracts using several databases: Google Scholar/PubMed, n=198 and the China National Knowledge Infrastructure (CNKI) and Chinese Biology Medicine disc (CBMdisc), n=653, as shown in Supplementary Figure S1. Searches with Embase and the Cochrane Library did not yield any eligible trials. After 193 duplicates were excluded, 658 abstracts were screened and 194 were excluded during initial screening (reviews, meta-analyses, guidelines, meeting abstracts, animal model studies, etc.) and 464 full articles were reviewed. Of these, 274 did not meet inclusion criteria (for example, non-PAGE patients were enrolled, n=123, or S. boulardii was used in both control and intervention groups, n=73). Of 190 trials of PAGE, 180 were excluded when additional treatments were used in the trial such as zinc, montmorillonite, racecadotril, etc. (n=109), the control group another type of probiotic (n=39), the strain of S. boulardii was not described (n=23), outcome was not diarrhea (n=6) or the study intervention included both S. boulardii and another type of probiotic (n=3). Examples of nine trials excluded for commonly found reasons are provided in Supplementary Table S3 (Li et al., 2014; Duan et al., 2017; Vineeth et al., 2017; Wang et al., 2017; Zhao et al., 2017; Feng et al., 2018; Liu, 2020; Mourey et al., 2020; Altcheh et al., 2022). A total of 10 trials were included in our review (1125 participants) (Qu, 2012; Chen, 2014; Lv, 2014; Tan, 2015; Yang, 2015; Cao et al., 2017; Qiu, 2018; Yao, 2018; Chen, 2020; Wu, 2021). All Chinese articles were translated into English. Publication bias was assessed for the ten RCTs and found publication bias was present (p=0.016), due to more trials with a higher effectiveness rating, as shown in Supplementary Figure S2.
Study participant characteristics
The characteristics of the trials and study participants are provided in Supplementary Table S4. All trials were conducted in children (<1 month to 6 years old) with acute diarrhea. The children were enrolled shortly after the onset of the diarrhea: <2–5 days (7, 70%), <15 days (1, 10%), but the specific day of diarrhea onset was not reported in two trials (Qu, 2012; Wu, 2021). The most identified etiology of PAGE was rotavirus (4, 40%) (Chen, 2014; Tan, 2015; Yang, 2015; Chen, 2020), one trial enrolled children with bacterial diarrhea (1, 10%) (Qiu, 2018) and 5 (50%) did not report PAGE etiology. Most of the children were inpatients (9, 90%) and one trial enrolled only outpatients (Chen, 2014).
Study design
The study size ranged widely from 84–188 participants/trial (mean 112.5 ± 33.4), as shown in Supplementary Table S4. All ten trials provided conventional therapies as needed to both S. boulardii and control groups. All used open controls and none used a placebo. None of the trials reported lost-to-follow-up or attrition in their trials.
Only one trial had a high risk of bias (Tan, 2015), nine were rated as having some concerns and none had a low risk of bias. The most common reasons for low scores were missing items relating to the method of randomization (5, 50% did not provide this data), 100% were not blinded (allocator, outcome assessor and study staff), failure to provide a sample size calculation and failure to discuss limitations, generalization, and comparison with other studies in the discussion. The trial rated with a high risk of bias also did not report a comparison of baseline characteristics in their trial (Tan, 2015). The risk of bias was similar for the domains in most trials, as shown in Supplementary Table S5.
Intervention characteristics
All trials identified the strain of probiotic used as S. boulardii CNCM I-745 (Biocodex or ‘Yihuo’) in a sachet. The duration of treatment ranged from 3 days (n=4, 40%), 4–5 days (n=2, 20%) to 7 days (n=3, 30%) but was not reported in one trial (Wu, 2021). None of the trials followed patients after the study intervention was discontinued. The dose of S. boulardii ranged from 125 mg/d to 500 mg/d and 7 (70%) trials used different daily doses depending upon the age of the child, while two trials gave a consistent dose regardless of the age (500 mg/d) (Qiu, 2018; Wu, 2021) and one trial did not report the dose of S. boulardii given (Cao et al., 2017).
Primary outcome: reduction in duration of diarrhea
Overall, significant reduction in the duration of days of diarrhea was noted for S. boulardii CNCM I-745 compared to the controls (Table 1) in six RCT (Tan, 2015; Cao et al., 2017; Qiu, 2018; Yao, 2018; Chen, 2020; Wu, 2021) with the S. boulardii group having a mean duration of diarrhea 1.6 days less than the controls (SMD = -1.63 days, 95% CI -2.08, -1.18, z=- 7.05, p<0.0001, I2 = 84.8%), as shown in Figure 1. Cao et al. reported a very short duration of diarrhea, which could not be confirmed as no contact information was provided (Cao et al., 2017). When this trial was excluded from the analysis, the reduction in mean duration of diarrhea was not significantly different than when this trial was included (SMD: -1.67, 95% CI -2.26, -1.08). When a trial rated with high risk of bias was excluded from the analysis (Tan, 2015), the reduction of days of diarrhea by S. boulardii was similar in those trials with moderate rating of risk of bias (SMD= -1.46 days, 95% CI -1.87, -1.06, z=- 7.13, p< 0.001, I2 = 77.9%). Other sensitivity analyses found no one trial had an undue influence on the pooled efficacy estimates.

Table 1. Pediatric acute gastroenteritis (PAGE) outcome measures comparing S. boulardii CNCM I-745 and control groups.

Figure 1. Forest plot of the reduction in duration of PAGE diarrhea (days) comparing S. boulardii CNCM I-745 and control groups. CI, confidence interval; DL, DerSimonian-Laird estimate; Ref, reference.
Subgroup analysis by etiology of PAGE (rotaviral vs bacterial or not defined) showed a significant difference by etiology (Chi2 = 10.6, p=0.005), with a significantly shorter duration of diarrhea noted for those with rotaviral diarrhea (SMD= -2.10 days, 95% CI -2.78, -1.42, p=0.04, I2 = 75.1%). The effectiveness in bacterial etiology could not be determined as only one trial included this etiology (Qiu, 2018). A sub-group analysis by the daily dose of S. boulardii resulted in lower heterogeneity (Supplementary Figure S3). Most trials adjusted the dose (125–500 mg/d) depending upon the age of the child and two trials used a consistent dose (500 mg/d) regardless of the children’s ages. When the dose was age-adjusted, the duration of diarrhea was significantly reduced (SMD= -2.11 days, 95% CI -2.40, -1.83, I2 = 51.5%). When the dose was 500 mg/d, the duration of diarrhea was also shortened, but to a lesser extent (SMD= -1.02 days, 95% CI -1.32, -0.73, I2 = 18.7%). A sub-group analysis by the duration S. boulardii was given resulted in lower heterogeneity, but conclusions could not be drawn due to the limited number of trials with the same number of days of S. boulardii given (Supplementary Figure S4).
Primary outcome: total effectiveness rating
All ten trials reported the total effectiveness rating for their trials (Table 1), which is based on the improvement of diarrheal symptoms. The total effectiveness rating was significantly higher for those treated with S. boulardii compared to controls (RR=1.22, 95% CI 1.16, 1.28, z=7.7, p<0.001, I2 = 0%), as shown in Figure 2. When sub-group analysis was done for different etiologies of PAGE or duration of S. boulardii given, neither variable had a significant impact on the improvement of diarrhea symptoms. Different durations of S. boulardii given (from 3–7 days) did not significantly impact the effectiveness (Chi2 = 1.14, p=0.77). When the one trial with high risk of bias was excluded (Tan, 2015), the nine trials with ‘some concerns’ of risk also reported a higher effectiveness rating in those with S. boulardii compared to controls (RR= 1.22, 95% CI 1.16, 1.29, Z=7.28, p<0.001, I2 = 0%).

Figure 2. Forest plot of the total effectiveness rating for the improvement of diarrhea symptoms comparing S. boulardii CNCM I-745 and control groups. CI, confidence interval; MH, Mantel-Haenszel; Ref, reference.
All ten trials also reported the frequency of cure by the end of the study for their trials (Table 1) and significantly more of those treated with S. boulardii reported being cured compared to controls (RR= 1.47, 95% CI 1.30,1.67, z=6.04, p<0.0001, I2 = 30.9%). as shown in Figure 3. There was a significantly higher cure rate in those with rotaviral PAGE (RR=1.46, 95% CI 1.21, 1.75, p=0.03, I2 = 0%). The cure rate was similar regardless of the duration of S. boulardii given (from 3–7 days), Chi2 = 3.61, p=0.31.

Figure 3. Forest plot of the number cured of PAGE by study end comparing S. boulardii CNCM I-745 and control groups. CI, confidence interval; MH, Mantel-Haenszel; Ref, reference.
Primary outcome: reduction in stool frequency by end of treatment
A pooled analysis was not possible, as only one trial reported daily stool frequency at the end of treatment (Qiu, 2018). In this trial, those with treated with S. boulardii had significantly fewer mean stools per day on Day 3 (1.82 ± 0.54/day, p<0.05) compared to controls (2.93 ± 0.4/d) (Qiu, 2018).
Secondary outcome: safety
Eight (80%) of the trials reported safety data, while 2 (20%) failed to provide any safety data (Yang, 2015; Qiu, 2018), as shown in Supplementary Table 4. Most trials (50%) just provided a statement that ‘no adverse events were reported’, while three trials provided more details for the frequency of reported adverse events by study group (Yao, 2018; Chen, 2020; Wu, 2021). The risk of adverse events was significantly less in those given S. boulardii (RR=0.19, 95% CI 0.07, 0.50, p=0.001, I2 = 0%) compared to the controls, as shown in Supplementary Figure S5. Most of the adverse events were mild, self-resolving symptoms (rash, abdominal discomfort, dry mouth, nausea, vomiting, fever, constipation) and no serious adverse events were reported.
Secondary outcome: changes in immune markers
Three trials documented change in TNF-α levels from baseline to study end (Chen, 2014; Yang, 2015; Chen, 2020). Those treated with S. boulardii showed a significant reduction in mean TNF-α values compared to controls (SMD = -2.76, 95% CI -3.09, -2.43 z=-16.4, p<0.0001, I2 = 0%), as shown in Figure 4A. Three trials documented a significant reduction in IL-8 levels from baseline to study end (SMD= -11.2, 95% CI -15.6, -6.8, z=-12.43, p<0.001, I2 = 98.9%), as shown in Figure 4B (Yang, 2015; Qiu, 2018; Chen, 2020). Although four other trials (Chen, 2014; Cao et al., 2017; Qiu, 2018; Chen, 2020) reported significant changes in other immune markers (CD4/CD8 ratios, CRP, CD3, IL10) for S. boulardii compared to controls, no two trials reported the same immune markers so these could not be analyzed (see Supplementary Table S6).

Figure 4. Forest plots of: Top (A) Changes in TNF-alpha levels in S. boulardii CNCM I-745 compared to controls, Bottom (B) changes in IL-8 levels in S. boulardii CNCM I-745 compared to controls. CI, confidence interval; DL, DerSimonian-Laird estimate; IV, inverse variance estimate; Ref, reference; SMD, standardized mean difference.
Other secondary outcomes
Several secondary outcomes could not be assessed due to the limited number of trials reporting those outcomes. Only two trials reported time to cessation of vomiting (Chen, 2020; Wu, 2021), only one trial reported time to cessation of nausea (Chen, 2020) and only trial reported the length of hospitalization (Yao, 2018).
Discussion
Our review found S. boulardii CNCM I-745 significantly reduced the duration of PAGE, significantly improved diarrheal symptoms and had a higher cure rate compared to controls in patients in China. This is the first meta-analysis that identified multiple trials in China for this condition using a defined probiotic (S. boulardii CNCM I-745). Previous meta-analyses of S. boulardii for PAGE treatment did not include any trials from China (Feizizadeh et al., 2014; Szajewska et al., 2020; Huang et al., 2021; Juangco et al., 2021; Li et al., 2021; Fu et al., 2022), which may be due to reliance on standard literature databases, which do not typically include trials published in Chinese language journals. All our included trials were identified in Chinese literature databases and were not detected in Google Scholar or PubMed literature searches.
One challenge in determining the efficacy of probiotics for PAGE treatment in our meta-analysis was that there were multiple types of outcome measures used in our meta-analysis: 100% reported effectiveness rating, 60% reported duration of diarrhea, while only one reported changes in daily stool frequency (Qiu, 2018). The trials done in China reported an outcome measure not typically reported in trials in other countries: Total Effectiveness Rating, which measured the improvement of diarrheal symptoms and not just the duration of days of diarrhea. Meta-analyses done in trials from other countries have also used different types of outcomes (duration of diarrhea, daily stool frequency, vomiting, etc.) and while duration of diarrhea is the most commonly reported outcome, it was not found in all our trials (Szajewska et al., 2020; Huang et al., 2021; Juangco et al., 2021; Li et al., 2021; Fu et al., 2022). A large meta-analysis of 84 RCT of probiotics for PAGE found 14% did not report this outcome (Li et al., 2021) and similarly another meta-analysis of 29 RCT using S. boulardii for PAGE reported 29% did not report duration of diarrhea as an outcome (Szajewska et al., 2020), rather other outcomes were reported. As there is no current consensus on which outcome measure should be the gold standard for PAGE, different outcomes will continue to be challenging when a meta-analysis is performed. Our meta-analysis did find a slightly greater reduction in the mean duration of PAGE diarrhea (-1.63 days) in the trials done in China compared to trials done in other countries (ranging from -0.7 to -1.25 days reduction). One possible explanation for the greater reduction in effect in our study may be that other meta-analyses have included trials that included controls with other anti-diarrheal therapies including zinc or other probiotics (Feizizadeh et al., 2014; Szajewska et al., 2020; Fu et al., 2022), while our controls did not include these adjunctive therapies. Our meta-analysis excluded trials that used other additional treatments (such as montmorillonite or traditional Chinese medicines not typically used in non-Chinese treatments of PAGE) in an effort to have comparable therapies so efficacy could be compared when S. boulardii is used in Chinese versus other countries. When S. boulardii is added to conventional rehydration therapy, a similar degree of efficacy is seen for the reduction of diarrhea when compared to trials done in other countries.
There are several mechanisms-of-action for S. boulardii CNCM I-745 which may help to explain the efficacy for the treatment of PAGE including immune regulation, competition with pathogen attachment sites, production of anti-toxin proteases and anti-secretory effects (Buccigrossi et al., 2014; Stier and Bischoff, 2016; Czerucka and Rampal, 2019). Our meta-analysis documented that S. boulardii CNCM I-745 was especially effective for reducing the duration of diarrhea caused by rotavirus and this may be due to the ability of this strain to reduce the secretion of chloride (Buccigrossi et al., 2014; Czerucka and Rampal, 2019). Our meta-analysis is the first to our knowledge reporting on the impact of S. boulardii CNCM I-745 on immune cytokines in children with PAGE. One mechanism for the efficacy of S. boulardii involves the regulation of immune cytokines, but not all trials collected information on immune markers and of those that did, the types of immune markers assessed were not consistent with each other. Thus, only two cytokines (TNF-α and IL8) were able to be assessed. Previous meta-analyses focused on diarrheal outcomes of PAGE, but did not explore this mechanism (Feizizadeh et al., 2014; Szajewska et al., 2020; Huang et al., 2021; Juangco et al., 2021; Li et al., 2021; Fu et al., 2022).
Of the ten trials done in China in children with PAGE, none reported significantly more adverse events in those given S. boulardii CNCM I-745 and no serious adverse events were reported. Previous reviews have also reported that S. boulardii CNCM I-745 is well-tolerated in children but also noted that not all trials reported safety data (Feizizadeh et al., 2014; Szajewska et al., 2020).
Our meta-analysis has several strengths, including a robust literature review and use of standardized recommendations for reporting probiotic meta-analyses (McFarland et al., 2023). Use of Chinese databases for clinical trials uncovered a wealth of articles not detected in non-Chinese databases (Google Scholar and PubMed, etc.) and the ability of one of the co-authors to accurately translate these studies from the original Chinese language was invaluable. Another strength was our ability to account for strain-specificity important when analyzing the efficacy of probiotics by limiting our inclusion to one defined strain of S. boulardii CNCM I-745. Not all probiotic strains are alike and strain-specific effects for various diseases have been clearly demonstrated (McFarland et al., 2018). Strain-specificity has also been observed with other types of probiotics used for the treatment of PAGE (McFarland et al., 2021; Zhao et al., 2021; Chen et al., 2023; Chen et al., 2024). Another strength is that most of the trials used the same definitions for outcome measures and inclusion criteria using standardized definitions.
Our meta-analysis has several limitations. We found the reported clinical trials in China were of moderate quality, mainly due to the lack information about the method of randomization, the lack of blinding of study personnel and study participants, lack of placebo use, and limited information provided on recruitment procedures. The lack of placebo use in the trials done in China may have introduced a level of bias, but open controls using conventional therapies for PAGE have also been used in other countries. In the meta-analysis of Li et al., 42% of the 84 RCTs did not use placebos and in the meta-analysis by Szajewska et al., 62% of 29 RCTs did not use placebos (Szajewska et al., 2020; Li et al., 2021). The lack of blinding or use of placebos should be considered and evaluated but may not have had a large impact on these trials. One meta-analysis of 29 trials assessed the impact of placebo versus open controls and found a slightly larger reduction of the mean duration of diarrhea in 7 trials when S. boulardii was compared to placebo controls (MD: -1.24 days, 95% CI -1.74, -0.70) than when S. boulardii was compared to open controls in 16 RCTs (MD: -0.98 days, 95% CI -1.29, -0.68) (Szajewska et al., 2020). Another limitation was the inability to identify all the sources of heterogeneity due to the limited number of trials that reported data that might have been used in sub-group analyses. We were able to reduce the degree of heterogeneity for duration of diarrhea when controlling for several factors, including the dose of S. boulardii used and for also showing S. boulardii CNCM I-745 was more effective in children with rotaviral PAGE. When the overall quality of the trials are examined, several outcomes have a low GRADE recommendation (see Supplementary Table S7). Duration of diarrhea has high heterogeneity (85%) as only six trials reported this outcome, but the degree of variance was reduced when only rotaviral diarrhea was analyzed. The two immune markers (TNF-α and IL-8) have a low GRADE rating, as only three trials reported each of these outcomes. Two outcomes (Total Effectiveness Rating and Cure rate) were rated as moderate GRADE, due to low heterogeneity found for all 10 trials. However, as none of the trials were blinded, potential bias may exist. Another limitation is that only 10 trials met our inclusion criteria, which is also observed when other meta-analyses have restricted their meta-analysis to one type of probiotic or one country to reduce heterogeneity (McFarland et al., 2021; Fu et al., 2022).
Future meta-analyses of probiotics could be improved by following recommendations from recognized experts in the field, including a clear definition of the strain(s) of probiotics assessed and not pooling different types of probiotics together in their analysis (McFarland et al., 2023). The quality of a meta-analysis is dependent upon the quality of the individual trials, so efforts to improve individual trials would be beneficial for future meta-analyses. The low-moderate quality in these trials was typically due to not following CONSORT guidelines for reporting clinical trials including a lack of a complete description of the randomization method or stating how allocation assignments were kept blinded (Moher et al., 2010). Future clinical trials need to report follow-up post-study intervention and use blinded interventions (study staff and study participants), as the use of placebo with standard treatments would reduce possible bias. Use of standardized definitions of primary outcome measures is recommended, as multiple definitions were used (antidiarrheal time, time to diarrhea resolution, etc.). In order to assess if immune regulation is involved in PAGE with S. boulardii, future trials should collect consistent data on the same type of immune markers. Although we were able to only include a limited number of trials (n=10) due to strict inclusion/exclusion criteria, the finding there are numerous trials using additional treatments (such as montmorillonite, racecadotril or traditional Chinese medicines) not typically found in non-Chinese trials may be of interest to examine in future studies.
As this analysis was based on trials limited to one country (China), it would be prudent to consider how the generalizability of these efficacy results might vary in countries with different diets, health status and lifestyles or differences due to study design and choice of controls. Implications for clinical practice and healthcare policy of this meta-analysis is S. boulardii CNCM I-745 is promising for the treatment of PAGE.
This meta-analysis revealed numerous clinical trials that were undiscovered by prior meta-analyses of S. boulardii for the treatment of PAGE by utilizing country-specific literature databases. Our meta-analysis found S. boulardii CNCM I-745 is an effective and safe treatment for children with PAGE in China.
Data availability statement
The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding author.
Author contributions
LM: Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Visualization, Writing – original draft, Writing – review & editing. TL: Data curation, Investigation, Methodology, Supervision, Validation, Writing – original draft, Writing – review & editing.
Funding
The author(s) declare that financial support was received for the research and/or publication of this article. The authors declare financial support was received for the research, and publication of this article. This study was supported by Biocodex, France. The sponsor had no direct involvement with the decision to publish and neither author has equity or stocks in Biocodex.
Conflict of interest
Author LM was employed by McFarland Consulting and Public Health Reserve Corps. LM is a consultant for Biocodex France and Axon Pharma SAS South America and Dr. Reddy’s Laboratory India and is on the Microbiome Advisory Board Biocodex, France.
The remaining author declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Generative AI statement
The author(s) declare that no Generative AI was used in the creation of this manuscript.
Publisher’s note
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Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2025.1587792/full#supplementary-material
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Keywords: Saccharomyces boulardii, pediatric, acute diarrhea, meta-analysis, probiotic
Citation: McFarland LV and Li T (2025) Efficacy and safety of Saccharomyces boulardii CNCM I-745 for the treatment of pediatric acute diarrhea in China: a systematic review and meta-analysis. Front. Cell. Infect. Microbiol. 15:1587792. doi: 10.3389/fcimb.2025.1587792
Received: 04 March 2025; Accepted: 09 May 2025;
Published: 04 June 2025.
Edited by:
Ke Chen, University of Electronic Science and Technology of China, ChinaReviewed by:
Bantayehu Addis Tegegne, Debre Markos University, EthiopiaZhihong Liang, China Agricultural University, China
Copyright © 2025 McFarland and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Lynne V. McFarland, bWNmYXJsYW5kLmx5bm5lLnZAZ21haWwuY29t
†These authors have contributed equally to this work