ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Veterinary and Zoonotic Infection
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1609022
This article is part of the Research TopicUnveiling Host-Pathogen Interactions: Insights into Animal Cellular Immunity and Novel Diagnostics - Volume IIView all 6 articles
Establishment of Infectious Clone of Porcine Transmissible Gastroenteritis Virus and Study on the Location and Function of Accessory Protein 3
Provisionally accepted
Zhenye Hao1,2Lifei Liu1,2Shuai Wang1,2Yanping Jiang1,2Jiaxuan Li1,2Wen Cui1,2
Li Wang1,2*Yijing Li1,2*- 1College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
- 2Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Northeast Agricultural University, Harbin, Heilongjiang Province, China
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Transmissible gastroenteritis virus (TGEV) as causative agent of TGE causes huge economic losses in the pig industry worldwide. In this study, we successfully constructed a reverse genetic system for the TGEV TH-98 strain and rescued the recombinant virus rTH-98-Δ3-COE-HA based on this system. The results showed that ORF3 is a non-structural protein and does not exist in mature virions. Therefore, the rTH-98-Δ3-COE-HA could induce inflammatory cytokine expression in IPEC-J2 cells, but the expression levels were significantly lower than those triggered by TH-98 and rTH-98, indicating that TGEV ORF3 may play a critical role in inducing the host immune response. To detect whether the exogenous protein expressed in the rTH-98-Δ3-COE-HA has immunogenicity, the results showed that the levels of antibodies were significantly increased in mice. rTH-98-Δ3-COE-HA can induce a specific immune response in host body against its expressed exogenous proteins. Our study supplied important clues for revealing the role of ORF3 in virus replication and pathogenesis, laying a theoretical and material foundation for the construction of recombinant viral multi-valent vaccines.
Keywords: TGEV, ORF3, Reverse Genetics, viral replication, Immunogenicity
Received: 09 Apr 2025; Accepted: 14 May 2025.
Copyright: © 2025 Hao, Liu, Wang, Jiang, Li, Cui, Wang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Li Wang, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
Yijing Li, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
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