ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Clinical and Diagnostic Microbiology and Immunology
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1609666
Assessment of a Rapid Diagnostic Test Based on Loop-mediated Isothermal Amplification to Identify the Most Frequent Pathogens Causing Hospital-Acquired Pneumonia
Provisionally accepted
Jordi Vila1*
Anna Sellares-Crous1
Arturo Martínez-Trejo2Natalia Arnalda-Muñoz1Giulia Gatti2Miriam Villanueva-López1
ANDREA VERGARA1
Francesc Marco1Mateu Espasa Soley1- 1Clinical Microbiology, Hospital Clinic of Barcelona, Barcelona, Spain
- 2Instituto Salud Global Barcelona (ISGlobal), Barcelona, Catalonia, Spain
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Hospital-acquired pneumonia (HAP) is a serious infection affecting patients in the hospital setting.This study aimed to evaluate a novel multiplex detection method using loop-mediated isothermal amplification (LAMP) technology to identify six primary bacterial pathogens responsible for HAP directly from respiratory samples. A total of 119 clinical samples were analyzed by LAMP technology, including mainly bronchoalveolar lavages, endotracheal aspirates, and bronchoaspirates. The results of the LAMP and traditional culture methods showed an accuracy of 93.0%. In some discordant cases among culture and LAMP, multiplex-PCR (Filmarray Pneumonia Panel) showed a strong correlation with LAMP results confirming the potential use of this technique as a diagnostic detection tool. The clinical sensitivity of the LAMP assay was 93.3% with a specificity of 92.0%. Correlation analysis revealed a weak negative relationship between bacterial load and time to positivity (r=-0.177, p=0.05).This study underscores the potential of LAMP as a rapid and accurate tool for the diagnosis of HAP, facilitating the turnaround time for microbiology laboratory results, which is critical for improving the outcomes of patients with HAP.
Keywords: Hospital-acquired pneumonia, LAMP, Rapid diagnostic test, Pathogen Detection, respiratory samples. (Min.5-Max. 8
Received: 10 Apr 2025; Accepted: 12 Aug 2025.
Copyright: © 2025 Vila, Sellares-Crous, Martínez-Trejo, Arnalda-Muñoz, Gatti, Villanueva-López, VERGARA, Marco and Espasa Soley. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jordi Vila, Clinical Microbiology, Hospital Clinic of Barcelona, Barcelona, Spain
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