ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Clinical and Diagnostic Microbiology and Immunology
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1611134
This article is part of the Research TopicOmics Sciences in Microbiology and Infectious DiseasesView all articles
A rapid and accurate method for Helicobacter pylori detection via integrating LAMP assay with CRISPR/Cas12b detection by one-step in one-pot
Provisionally accepted
- Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
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Accurate and timely detection of Helicobacter pylori (HP) is critical for the diagnosis and management of gastritis-related diseases. In this study, we developed a novel integrated detection platform by combining loop-mediated isothermal amplification (LAMP) with the CRISPR/Cas12b system in a one-step, one-pot reaction for HP identification. This method enables rapid and sensitive HP detection within 45 minutes at a constant temperature of 58 ℃, eliminating the need for sophisticated instrumentation or specialized technical expertise. The system demonstrated a limit of detection (LOD) of 14.77 copies per test, with no cross-reactivity observed against potential interfering nucleic acids, confirming 100% specificity for HP. Clinical validation 22 samples revealed a 90.91% concordance rate (20/22) between the LAMP-CRISPR/Cas12b assay and conventional PCR, underscoring its diagnostic reliability. Our findings not only present a robust and efficient platform for HP detection but also provide a solid foundation for the development of point-of-care testing (POCT) devices and in vitro diagnostic tools for clinical use. Keywords: Helicobacter pylori, CagA, LAMP, CRISPR/Cas12b, one-step, one-pot
Keywords: Helicobacter pylori, CagA, LAMP, CRISPR/Cas12b, One-step, One-pot
Received: 13 Apr 2025; Accepted: 31 Jul 2025.
Copyright: © 2025 Zhang, Liu, Zhang, Ni, Wang, Bai, Sun, Guan, Xia, Cao and Gu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jiayi Gu, Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
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