ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Molecular Bacterial Pathogenesis
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1627518
The RNA-Binding Protein ProQ Directly Binds and Regulates Virulence Genes in Enterohemorrhagic Escherichia coli O157:H7
Provisionally accepted
- 1School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China
- 2School of Public Health, North China University of Science and Technology, Tangshan, China, 063210, Tangshan, China
- 3University of Manitoba, Winnipeg, Canada
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Abstract ProQ has recently emerged as a major post-transcriptional regulator in bacteria through directly binding to diverse mRNAs and small regulatory RNAs (sRNAs). However, the impact of ProQ in many pathogenic bacteria remains poorly understood. In this study, we investigated the role of ProQ in enterohaemorrhagic Escherichia coli (EHEC), a non-invasive intestinal pathogen. We found that deletion of proQ significantly enhanced cell adherence ability of EHEC and led to upregulation of the locus of enterocyte effacement (LEE) pathogenicity island. This effect was accompanied by reduced expression of genes encoding known LEE regulators, including protein factors (ihfA and hns) and sRNAs (GlmY and GlmZ), resulting from decreased stability of their transcripts in the absence of proQ. Additionally, proQ positively regulated bacterial motility by stabilizing fliC mRNA. We further demonstrated that ProQ directly binds to ihfA, hns, glmY/glmZ and fliC transcripts at secondary structures located near their 3' ends. Beyond virulence regulation, ProQ also contributed to antibiotic persistence of EHEC and its survival under host-associated stress conditions. Collectively, our findings highlight ProQ as a key gene regulator in EHEC, providing new insights into how this pathogen modulates its virulence at the post-transcriptional level.
Keywords: RNA regulation, EHEC, O157:H7, RNA binding protein, PROQ, small regulatoryRNA, virulence gene regulation, LEE
Received: 12 May 2025; Accepted: 15 Aug 2025.
Copyright: © 2025 Qian, Zheng and Han. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Chenguang Zheng, School of Public Health, North China University of Science and Technology, Tangshan, China, 063210, Tangshan, China
Runhua Han, University of Manitoba, Winnipeg, Canada
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