ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Veterinary and Zoonotic Infection
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1631048
This article is part of the Research TopicEmerging and re-emerging vector-borne diseases: A global challengeView all 4 articles
Establishment of reverse transcription recombinase-aided amplification with lateral flow dipstick for the rapid visual detection of Getah virus
Provisionally accepted
- China Agricultural University, Beijing, China
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Getah virus (GETV) is a globally spreading zoonotic mosquito-borne virus that primarily affects horses and pigs, causing significant economic losses in the livestock industry. Therefore, there is an urgent need for improved diagnostic methods to manage future outbreaks. In this study, we developed a nucleic acid detection assay, reverse transcription recombinase-aided amplification with lateral flow dipstick (RT-RAA-LFD), for the rapid and convenient detection of GETV. The RT-RAA-LFD assay could be completed at 40℃ for 15 min. Under optimal reaction conditions, the assay demonstrated excellent specificity, with no cross-reactivity observed with other clinically relevant swine pathogens. It achieved a broad detection range and a limit of detection (LOD) of 5.53 × 10 2 copies/μL, which was lower than that of RT-PCR (5.53 × 10 3 copies/μL) assay and slightly higher than that of qRT-PCR (5.53 × 10 1 copies/μL) assay for GETV. When testing 21 blood samples, the results of RT-RAA-LFD were fully consistent with those of the RT-PCR and qRT-PCR assays. In testing 45 tissue samples, the Kappa value for consistency between RT-RAA-LFD and RT-PCR was 0.776 (P < 0.001), with a concordance rate of 95.6% (43/45). The Kappa value for consistency between RT-RAA-LFD and qRT-PCR was 0.845 (P < 0.001), with a concordance rate of 97.8% (44/45). In conclusion, the RT-RAA-LFD assay shows great potential as a efficient and user-friendly diagnostic tool for GETV screening, particularly in laboratories with limited resources and equipment.
Keywords: Getah virus, Arbovirus, Recombinase-aided amplification, Lateral flow dipstick, Nucleic acid detection
Received: 19 May 2025; Accepted: 25 Jul 2025.
Copyright: © 2025 Wei, Xing, Sun, Zhang, Dong and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yanjun Dong, China Agricultural University, Beijing, China
Hanchun Yang, China Agricultural University, Beijing, China
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