ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Clinical and Diagnostic Microbiology and Immunology
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1638464
This article is part of the Research TopicAdvances in Vaginal Microbiome and Metabolite Research: Genetics, Evolution, and Clinical PerspectivesView all 14 articles
Mixed vaginal infection status in women infected with Trichomonas vaginalis: Comparison of microscopy method and Metagenomic sequencing analysis
Provisionally accepted
- 1First Hospital, Peking University, Beijing, China
- 2Chinese Academy of Agricultural Sciences Agricultural Genomics Institute at Shenzhen, Shenzhen, China
- 3Guangdong Research Institute of Genetic Diagnostic and Engineering Technologies for Thalassemia, guangdong, China
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Trichomonas vaginalis (TV) infection is one of the most common non-viral sexually transmitted infections globally. It not only causes symptoms such as abnormal vaginal discharge, itchiness, and pain but also severely impairs patients' quality of life and significantly elevates the risk of other vaginal infections and related diseases. Currently, clinical diagnosis of TV primarily relies on wet film microscopy, which has limitations such as low sensitivity. In terms of treatment, nitroimidazoles are the first-line drugs, but they face challenges of increasing drug resistance and recurrence rate. Most existing studies have focused on single TV infections, with limited attention to mixed infections. However, mixed infections may induce more intense inflammatory responses, disrupt vaginal microbiota, and affect treatment selection and efficacy. In this study, we analyzed clinical samples from TV-infected patients to investigate vaginal microbiota changes and the diagnosis of mixed vaginal infections in the context of TV co-infection. The findings will help clinicians comprehensively assess patient conditions, optimize treatment regimens, and are of great significance for reducing the disease burden of trichomoniasis and its complications.
Keywords: Vaginal trichomoniasis, mixed vaginitis, vaginal microbiome, Community State Type (CST) classification, Metagenomic sequence
Received: 30 May 2025; Accepted: 24 Sep 2025.
Copyright: © 2025 Jun, Xiulin, Zhang, Zheng, Chen, Mi and Xiao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dai Zhang, mailzhangdai@sina.com
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