ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Clinical Infectious Diseases
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1640503
This article is part of the Research TopicDevelopment and Application of New Diagnostic Methods in Clinical Diagnosis of Virus-Related DiseasesView all 6 articles
Portable, Precise, and RNA Extraction-Free: RT-RAA Technology for Rapid Early RSV Identification and Prevention
Provisionally accepted
Zhenfei Wang1
Jing Zhang2Xiao He2Chunming Wang2Zhenyue Li3Zitong Yang3Cheng Zhang3Tingli Fan2*
Su Kai2*- 1Shandong University Cheeloo College of Medicine, Jinan, China
- 2Cangzhou Technical College, Cangzhou, China
- 3Hebei Agricultural University, Baoding, China
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The Respiratory Syncytial Virus (RSV) is a significant agent linked to respiratory infections, representing a considerable health risk for vulnerable populations, including infants, older adults, and those with weakened immune systems. This research successfully introduces an RNA extraction-free rapid detection technique for RSV utilizing real-time reverse transcription recombinase-aided amplification (RT-RAA) technology. Through the crafting of specific primers and probes, this approach enables precise identification of RSV without any interference from other prevalent respiratory viruses. Tests for sensitivity indicated that the detection threshold at a 95% confidence interval was 159 copies per reaction, while the visual detection limit was found to be 1,177 copies per reaction. Testing on clinical samples demonstrated a high degree of consistency with reverse transcription quantitative real-time PCR (RT-qPCR), achieving a Kappa value of 1, which signifies excellent correlation. Furthermore, the amplified products from RT-RAA can be seen with the aid of a portable blue light device, rendering this method appropriate for rapid detection in settings where resources are limited. A total of 265 clinical samples were tested, and the results showed 100% concordance with RT-qPCR. Compared with rapid antigen detection tests (RADTs), RT-RAA exhibited significantly higher sensitivity (100% vs. 93.8%). The rapid detection method for RSV using RT-RAA offers solid technical assistance for the early identification and prevention of RSV.
Keywords: respiratory syncytial virus, Real-time Reverse Transcription Recombinase-aided Amplification, visual detection, Rapid detection, RNA extraction-free
Received: 03 Jun 2025; Accepted: 18 Aug 2025.
Copyright: © 2025 Wang, Zhang, He, Wang, Li, Yang, Zhang, Fan and Kai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Tingli Fan, Cangzhou Technical College, Cangzhou, China
Su Kai, Cangzhou Technical College, Cangzhou, China
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