BRIEF RESEARCH REPORT article
Front. Cell. Infect. Microbiol.
Sec. Virus and Host
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1648576
This article is part of the Research TopicViral Pathogenesis and Host Defense: Understanding the Missing Links to Combat DiseaseView all 9 articles
Low temperature increases adenovirus replication via intracellular alkalization
Provisionally accepted
- Department of Respiratory Medicine, General Hospital of Northern Theatre Command, Shenyang, China
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Abstract The change of environmental temperature is responsible for respiratory tract viral infections during the colder months of the year. However, the effect of low temperature on the replication of virus in pulmonary epithelial cells is still elusive. In this work, we measured the change of intracellular pH (pHi), and the replication of adenovirus in A549 cells. We observed that an exposure of cells to cooler temperature (33℃) resulted in increases in both intracellular pH, and replication of adenovirus. In addition, the enhanced replication of adenovirus induced by 33℃ was attenuated by inhibition of glycolysis with either 2-Deoxy-D-glucose (2-DG) or PFK158. Moreover, oligomycin, a stimulator on the glycolytic flux, led to a significant increase in the viral replication at 37℃. Further experiments showed that low temperature-promoted virus replication and intracellular alkalization were efficiently inhibited by acidification of the extracellular medium. Taken together, these data suggest that intracellular alkalization and glycolysis caused by low temperature enhance adenovirus replication in host cell.
Keywords: Low temperature, Adenovirus replication, intracellular alkalization, Glycolysis, The common cold
Received: 17 Jun 2025; Accepted: 15 Sep 2025.
Copyright: © 2025 Sun, Zhang, Cao and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Junli Zhang, junli1022@163.com
Zhuang Ma, ma-tianyi@163.com
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