Abstract
Purinergic signaling in bone was first proposed in the early 1990s with the observation that extracellular ATP could modulate events crucial to the normal functioning of bone cells. Since then the expression of nearly all the P2Y and P2X receptors by osteoblasts and osteoclasts has been reported, mediating multiple processes including cell proliferation, differentiation, function, and death. This review will highlight the most recent developments in the field of purinergic signaling in bone, with a special emphasis on recent work resulting from the European Framework 7 funded collaboration ATPBone, as well as Arthritis Research UK and Bone Research Society supported projects.
INTRODUCTION
Purinergic signaling is the most primitive and ubiquitous cell-to-cell signaling system that exists (). It involves the energy molecule adenosine triphosphate (ATP) and its breakdown products being used outside of the cell to bind specific receptors called purinoceptors which then direct the action and fate of cells. Just as every living organism uses ATP for energy, nearly every cell will express purinoceptors and use purinergic signaling to control cellular functions (for a recent review, see ).
In the context of bone, purines and pyrimidines are present in the extracellular milieu, either as a consequence of lytic or controlled release. Upon binding specific cell surface purinoceptors expressed by the different cell types in bone, intracellular calcium signaling cascades are triggered to direct the fate of bone cells and ultimately control bone homeostasis. Regulation of purinergic signaling in bone involves the co-ordinated actions of (1) controlled release of purines and pyrimidines from bone cells; (2) the expression and activity of enzymes on the surface of bone cells that can breakdown or interconvert ATP and other nucleotides; (3) purinoceptor expression by bone cells which is tightly controlled in a temporal and spatial manner.
Purinergic signaling in bone was first proposed in the early 1990s with the observation that extracellular ATP could raise intracellular calcium and induce secondary messenger activation – events crucial to the normal functioning of osteoblasts (; ). Since then it has been reported that osteoblasts and osteoclasts (of one species/type or another) express nearly all of the P2Y and P2X receptors, mediating multiple cellular processes and providing a mechanism to integrate local (i.e., ATP) and systemic (i.e., PTH) signaling to control bone turnover. For an extensive historical reviews of purinergic signaling in bone, see , , , , , and .
This review will discuss the most recent developments in the field of purinergic signaling in bone, highlighting work from a recent European Framework 7 funded collaboration called ATPBone, as well as Arthritis Research UK and Bone Research Society funded projects (see Figure 1 for a summary).
FIGURE 1
MODULATION OF ATP RELEASE FROM BONE CELLS
Bone is subjected to a wide array of mechanical stimuli that include fluid shear stress which occurs when the interstitial fluid within the canaliculi of bone flows past the cells of bone in response to movement, substrate strain, and compressive loading (; ; ). It is well established that mechanical stimulus applied to cells in vitro, even as subtle as fluid movements after a medium change, can increase basal ATP release (). A role for ATP release in the process of mechanotransduction in bone has been suggested for some time now (), although, as with the original concept of purinergic signaling, it is not yet widely acknowledged or appreciated.
Progress in unraveling the processes behind ATP release from osteoblasts has been advanced with results from a recent study comparing the effects of all of the loading forms experienced by bone including fluid flow, fluid shear stress, substrate strain, and 3D compressive loading (). ATP release was demonstrated to increase rapidly in response to turbulent fluid flow, with a gradual increase in response to fluid shear stress and substrate strain. A functional consequence of mechanical load-induced ATP release was shown to be the activation of the osteogenic immediate early gene c-fos, which was demonstrated to be activated most in response to 3D fluid flow and substrate strain. The results of this study, which was partly funded by a Bone Research Society Barbara Mawer Travelling fellowship, demonstrated that the amount of ATP released in response to mechanical loading varied in a time, direction, and strain-dependent manner and that this may represent a local mechanostat in bone that may influence osteogenesis.
Until recently, research on ATP release from bone cells has focused on osteoblasts, with a few reports on release of ATP from osteocytes (). A recent report highlights the importance of the voltage-sensitive calcium channel α2δ1 auxiliary subunit in regulating mechanically induced ATP release in the osteocytic cell line MLO-Y4 () offering further insights into the mechanisms behind regulation of ATP release in response to mechanical load.
Exciting new data has also emerged on the release of ATP from osteoclasts. In an oral presentation at the 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society, demonstrated for the first time that primary human osteoclasts release ATP and that active osteoclasts have an enhanced sensitivity to fluid flow. Until then little, if anything, had been reported on whether osteoclasts were capable of releasing ATP either constitutively or in response to mechanical loading. The concept that osteoclasts may be directly involved in sensing mechanical loading in bone via ATP release is further supported by recent data from reported in this research topic. They provided evidence for the involvement of the mechanosensitive P2X7 purinoceptor in the release of ATP from murine osteoclasts. Taken together, these two reports provide an intriguing new direction of research in bone mechanotransduction.
RECENT ADVANCES RELATING P2X7 RECEPTOR ACTIVITY AND OSTEOCLAST MULTINUCLEATION
The P2X7 receptor was originally discovered as the P2Z receptor, sensitive to high levels of ATP and thought to function only in the cytotoxic effect of extracellular ATP () – earning the receptor the nickname “the suicide receptor”! Many advances have been made in elucidating the role of this receptor since this initial proposed function (which surely would have evolved it to extinction if only involved in suicide?) including the release of cytokines and more contentiously cell fusion (). Both these proposed functions play key roles in bone homeostasis and accordingly several labs have investigated the role of the P2X7 receptor in bone cells, and more specifically in osteoclast fusion and multinucleation: in vitro blockade of the receptor prevents fusion (), whilst the P2X7R KO mice maintain the ability to form multinucleated osteoclasts in vitro and in vivo (; ; for review see ).
Multinucleation of osteoclasts is similarly a contentious issue – it probably still remains a debate as to whether multinucleation occurs to increase the efficiency of resorption, possibly via increased transcriptional activity (), or not (; ). Regardless of the reason for multinucleation of osteoclasts, the role of the P2X7 receptor in this process has been strengthened recently. Using a number of different P2X7 receptor antagonists, demonstrated that all but one compound decreased the formation and function of multinucleated TRAP-positive osteoclasts in a concentration-dependent manner. Whilst this study provides further evidence for the involvement of the P2X7 receptor in fusion of osteoclasts, it did not elucidate the mechanism by which P2X7 receptors are involved in fusion. This was however, addressed in another study by in which they proposed that P2X7 receptors are absolutely required for fusion of osteoclasts due to P2X7 receptor-dependent release of ATP which is then broken down to adenosine with the subsequent activation of adenosine receptors resulting in cell fusion. The role of P2X7 receptor in ATP release-driven cell fusion was also demonstrated to be critical for the induction of multinucleated macrophages by the inflammatory cytokine GM-CSF (). Targeting the P2X7 receptor in diseases with increased osteoclast multinucleation, such as Paget’s disease, may provide new therapeutic options.
MOUSE MODELS TO DETERMINE FUNCTIONAL CONSEQUENCES OF PURINERGIC SIGNALING IN BONE
Since the first report over 20 years ago that extracellular ATP could modulate intracellular calcium and second messenger signals in bone (; ) numerous groups have reported the expression of P2X and P2Y subtypes by the different bone cell types in a variety of species with wide ranging functional consequences (see reviews listed above for specific details). The most recent data to add to the catalog of expression and function profiles has emerged following the bone phenotype analysis of KO mice for P2Y6 receptor and the relatively recently discovered P2Y13 receptor.
As part of the EU Framework 7 funded project “ATPBone: Fighting osteoporosis by blocking nucleotides: purinergic signaling in bone formation and homeostasis,” the P2Y6 and P2Y13 receptor KO mice (P2Y6R– / – and P2Y13R– / –) were made available by Bernard Robaye and Jean Marie Boeynaems from the Institute of Interdisciplinary Research in Human and Molecular Biology, Universtité Libre de Bruxelles. Using P2Y6R– / – bone marrow derived cells to generate osteoclasts in vitro, demonstrated a reduction in bone resorption compared to wild-type (WT) osteoclasts. This data complemented their observation that when UDP, the agonist for the P2Y6 receptor, was added to mature murine osteoclasts for the final 48 h of culture it significantly increased resorption by up to 60%. In the same study the authors examined the bones of 2-month-old P2Y6R– / – mice and found that there was a reduction in the number of osteoclasts on the surface of the endocortical and trabecular bone. One would predict that given the observed reduction in osteoclast resorptive capacity in vitro and osteoclast numbers in vivo that the P2Y6R– / – mice would have a high bone mass phenotype. However, the authors found no significant effect of receptor deletion on the amount or architecture of trabecular bone either in the long bones or vertebrae of the P2Y6R– / – mice, yet they did detect a significant increase in cortical bone volume and thickness. The reason behind this effect in a discrete bone compartment is not yet known, and whether the lack of an extreme bone phenotype is due to purinoceptor redundancy or possible apposing effects of P2Y6 receptor deletion on osteoblasts remains to be elucidated. The full publication of further studies using adult P2Y6R– / – mice under challenged conditions such as mechanical loading () may go some way to explain the enigma of these mice.
The P2Y13R– / – mouse had a more obvious bone phenotype that was somewhat unsurprising given the previous reports that ADP, the preferred agonist of the P2Y13R, is a powerful osteolytic agent (). In a study recently highlighted by the Editor of Molecular Endocrinology as “excellent examples of the relevance of basic science findings to clinical management of endocrine disorders” (), found that P2Y13R– / – mice had a 40% reduction in trabecular bone volume, a 50% reduction in both osteoblasts and osteoclasts on the surface on bone and an overall 50% reduction in bone remodeling in vivo. This reduced bone turnover maybe as a consequence of the reduced ratio of the key bone modulating molecules receptor activator of nuclear factor κ-B ligand and osteoprotegerin and reduced downstream RhoA/ROCK I signaling. Furthermore, the reduced bone turnover observed in the P2Y13R– / – mice served to protect them from ovariectomy-induced bone loss by up to 65%. These exciting results highlight a potential alternative drug target for the treatment of bone diseases with accelerated bone turnover such as estrogen deficiency-induced osteoporosis.
Indeed, considerable advances have been made in developing pharmaceutical modulators of the P2Y receptors. Clopidogrel (Plavix®), a P2Y12 antagonist, was the second most sold pharmaceutical drug in the world in 2010. The P2Y12 receptor was first noted to be expressed by bone cells by . Interestingly, recent reports in abstract form have described an osteopetrotic phenotype of the P2Y12R– / – mice (), with delayed osteoclast formation in vitro and decreased Rap1 phosphorylation in response to ADP stimulation in P2Y12R– / – cells (). These abstracts also suggest a role for the P2Y12R in pathological bone loss and we eagerly await the full manuscript.
Another recent development in purinergic signaling using mouse models has arisen thanks to Jørgensen and colleagues at the Research Center for Aging and Osteoporosis, Copenhagen University Hospital who have back-crossed the original Glaxo P2X7R– / – mouse on to the BALB/cJ strain (). The two original P2X7R– / – mouse models (maintained on mixed C56BL/6 backgrounds) have a natural P451L mutation in the cytoplasmic domain of the P2X7 receptor which confers drastically reduced sensitivity to ATP-induced pore formation. While these existing P2X7R– / – strains have similar and contradictory results in many tissues including bone (i.e., the ability to form multinucleated osteoclasts in vivo and in vitro and an altered bone phenotype, respectively) there have been many reports that support an important role for the P2X7 receptor in bone (). Therefore, analysis of this new P2X7R– / – BALB/cJ strain may offer further new insights into the role of the P2X7R without the possible confounding contribution of the natural P451L mutation. Initial results have demonstrated that these mice also maintain the capability to form multinucleated osteoclasts both in vivo and in vitro (). In addition, when examining the ability of different aged mice to form osteoclasts in vitro it appears that formation and function is enhanced in young and developing mice, but that in older mice this increased resorptive capacity of the P2X7R– / – osteoclasts was not as apparent (). Again, we look forward to the full report of the bone phenotype of this strain and the contribution of this receptor to osteoclastogenesis.
PURINERGIC SIGNALING AND BONE DISEASE
Exciting progress has also been made in establishing a role for purinergic signaling in maintenance of healthy bone via studies involving human patient data. The first set of studies involve the gene for the P2X7 receptor, P2XR7, which is known to be highly polymorphic with over 26 non-synonymous single nucleotide polymorphisms (SNPs) listed on the NCBI database (Build 131), six of which have been previously described as resulting in either loss or gain of P2X7 receptor function. An initial report from the lab of Jørgensen reported that two SNPs in the P2X7 receptor gene (P2XR7) were associated with an increased risk of fracture (). Since then new SNPs have been found and it has been shown that heterozygote combinations of various SNPs result in an enhanced effect on the receptor function than any individual homozygote SNP. Given these observations further analysis of the role of other P2XR7 SNPs as well as combinations of SNPs in bone and indicators of osteoporosis, specifically bone mineral density (BMD), bone loss, and fracture was clearly warranted.
We have recently published the results of our study using women from the Aberdeen Prospective Osteoporosis Screening Study (APOSS) which revealed that a single SNP in the P2XR7, the c.946A (p.Arg307Gln), was significantly associated with low BMD at the lumbar spine of women aged 45–54 years and at a follow-up visit between 7 and 9 years later. Further analysis showed that when women with one or more loss-of-function SNP were grouped together they had almost a 10-fold increase in the amount of bone loss per year compared to women who were WT at these positions (). This was the first description that a P2XR7 polymorphism is associated with BMD, a key determinant of vertebral fracture risk, in post-menopausal women. The involvement of the P2XR7 in regulating human bone mass was further highlighted with a complimentary article from our ATPBone collaborators (). By genotyping women from the Danish Osteoporosis Prevention Study (DOPS) cohort for the same functional P2XR7 SNPs they confirmed the p.Arg307Gln SNP as being important to bone health – as women with this SNP had 40% increased bone loss. They also confirmed the effect of multiple SNPs on bone mass – they found a clear association between the low-risk (high-P2X7 function) alleles and a low rate of bone loss and conversely, high-risk (reduced P2X7 function) alleles were associated with a high rate of bone loss. In addition, they found a gain-of-function SNP was associated with lower vertebral fracture incidence 10 years after the menopause. Taken together, these two independent studies provide strong and compelling evidence that the P2RX7 is involved in the regulation of bone mass and fracture risk, and may in the future, represent an early diagnostic tool for the management of osteoporosis.
In addition to being implicated in osteoporosis, polymorphisms in the P2XR7 have been suggested to be involved in other musculoskeletal complications including aseptic hip loosening and rheumatoid arthritis (RA). A Czech group reported that the loss-of-function P2RX7 SNPs are overrepresented among patients with total hip arthroplasty (THA) whilst the p.Arg307Gln allele was associated with greater cumulative hazard of THA revision (), although the authors acknowledged the limitations of their under-powered study and suggested that replication is needed. Similarly, a report using a small cohort from the Omani Arab population is suggestive that polymorphism at position c.1068 and c.1513 in the P2X7R gene might contribute to the pathogenesis of RA as they were over-represented in the RA group and significantly associated with the presence of rheumatoid factor and anti-MCV autoantibody in RA patients, respectively. Whereas neither the loss-of-function c.1096C > G (p.Thr357Ser) SNP or the gain-of-function c.489C > T (p.His155Tyr) SNP appeared to be a susceptibility gene locus for the development of RA (). Both the c.946A (p.Arg307Gln) and c.1729A (p.Ile568Asn) SNPs were not detected in the Omani Arab population, probably due to low numbers (MAF 0.01 and 0.03, respectively, in a Caucasian population). Again, the authors acknowledged that further clarification and replication in a larger cohort was warranted to confirm their findings.
Clearly, further replication of the above observations in RA and aseptic loosening are warranted. As are further analysis of large cohorts such as DOPS and APOSS for SNPs in other purinergic receptors such as the P2Y2 receptor, which has a role in bone homeostasis and is also known to have numerous functional polymorphisms associated with other human diseases (), are eagerly anticipated.
As stated earlier, purinergic signaling is the most primitive and ubiquitous cell-to-cell signaling system that exists, and one might worry that any drug developed specifically to target one particular receptor for one particular disease may have effects in other systems. The P2Y12 receptor is known to play an important role in platelet activation – and P2Y12 antagonists have proven therapeutic value in the treatment and prevention of coronary artery disease. As mentioned earlier, the P2Y12 receptor has previously been identified as being expressed on human osteoblasts () and an osteopetrotic phenotype of the P2Y12R– / – mice has been reported (), although the physiological consequence of P2Y12 receptor activation or inhibition in human bone is not known.
Taking these previous reports into consideration, investigated the use of platelet inhibitors including clopidogrel. In this study, they found that the inhibitors dipyridamole and acetylsalicylic acid were associated with increased fracture, whereas clopidogrel was not. Even though this was a large scale study where all patients sustaining a fracture during the year of 2000 from the Danish population were included (approximately 124,655 cases from 5.3 million individuals), the authors postulated that the lack of any association of fracture risk to clopidogrel was due to the relatively short duration of exposure the patients would have had as the drug was only licensed in July 1998. Given that the number of defined daily doses (DDD) of clopidogrel sold increased by a factor of 30 from 103,000 in 1999 to 3.4 million in 2003 in Denmark alone additional studies in newer data sets were clearly warranted. To that end, again used the Danish population to identify patients who were prescribed clopidogrel during the years 1996–2008 (n = 77,503) and three non-users, matched for age and gender (n = 232,510), for each clopidogrel-treated subject to test for an association between clopidogrel use and fracture incidence. Using this data set they found a biphasic response to clopidogrel use, whereby those patients who had low exposure to treatment with clopidogrel (<0.01 DDD) had a lower risk of fracture than non-users, whilst the remaining patients prescribed clopidogrel had increased overall fracture risk and increased risk of osteoporotic fractures, especially in subjects with a treatment duration of more than 1 year.
What is behind these associations has yet to be elucidated. It could be as a consequence of low-grade inflammation associated with atherosclerosis: inflammation is often accompanied by bone loss and subsequently osteoporosis, thus the inflammation related to cardiovascular disease could be a contributing factor to the bone loss in the clopidogrel-treated group.
Direct antagonism of P2Y12 receptor expressed by both osteoblasts and osteoclasts could also be a contributing factor. Antagonism of the P2Y12 receptor on both cell types would disrupt the balance of bone homeostasis modulating both bone formation and resorption. This has recently been confirmed by who demonstrated that clopidogrel treatment of osteoblast and osteoclast cultures in vitro resulted in reduced osteoblast activity and mineralization, an increase in adipocytes and reduced osteoclast formation, viability, and resorptive activity. One might speculate that the in vitro observations were due to a non-specific effect of clopidogrel, which is a pro-drug that is rapidly metabolized in the liver to form the active metabolite; whether this occurs in these cultures is undetermined. The authors address this in their manuscript by providing supporting in vitro evidence for a direct effect in the form of alterations in cAMP levels and mRNA for key osteoblastic genes in osteoblasts following clopidogrel treatment. However, they acknowledge that off target, non-P2Y12 receptor-mediated effects on osteoblasts in vitro cannot be fully discounted. They then provide further in vivo evidence that clopidogrel has a detrimental effect on bone by comparing the bone parameters of ovariectomized mice with ovariectomized mice treated daily for 4 weeks with clopidogrel. Clopidogrel-treated mice had exaggerated effects of ovariectomy including significantly increased bone resorption and decreased bone formation markers, as well as enhanced bone loss as evidenced by significantly larger reductions in BMD, trabecular bone volume, and trabecular number. Clearly, further studies into the exact mechanism of action of clopidogrel, and the newer and more potent anti-platelet drugs such as prasugrel and ticagrelor, on bone metabolism are both timely and necessary.
Finally, the role of purinergic signaling in cancer is gradually emerging (, but the role of ATP and P2 receptors in bone cancer and cancer-induced bone disease (CIBD) still relatively unexplored. The P2X7 receptor has been implicated in many cancer types and its antagonism proposed as a potential treatment (). Given its role in bone cells one would speculate that it would be involved in bone cancer or CIBD. To date, only one study has investigated purinergic signaling in bone cancer, specifically the P2X7 receptor in bone cancer pain. In this study, injected 4T1 cancer cells directly into the femur of the mice and then measured pain-related behaviors and also bone destruction and tumor burden. They demonstrated that P2X7R-deficient mice were still susceptible to bone cancer pain and compared to cancer-bearing WT mice had an earlier onset of pain-related behaviors. There was no apparent difference in the bone destruction or tumor burden between WT and P2X7R-deficient mice, although the authors note that due to the advanced stage of cancer there was not a reliable measure of tumor burden in their model. They suggest that differences between the two genotypes could not be ruled out and experiments exploring earlier time points to fully elucidate the role of the P2X7 receptor in bone cancer are warranted.
SUMMARY
Clearly, we have come a long way in purinergic signaling in bone since the first description almost 20 years ago and we have made significant advances in the past few years thanks to some great collaboration. The availability of more KO (and transgenic) models as well as the development and use of specific antagonists for other conditions will undoubtedly help us to elucidate further the exact roles purinergic signaling pathways have to play in bone homeostasis, both in health and disease.
Conflict of Interest Statement:
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Summary
Keywords
ATP, purinergic signaling, P2Y, P2X, osteoblast, osteoclast, bone, osteoporosis
Citation
Rumney RMH, Wang N, Agrawal A and Gartland A (2012) Purinergic signalling in bone. Front. Endocrin. 3:116. doi: 10.3389/fendo.2012.00116
Received
29 April 2012
Accepted
04 September 2012
Published
19 September 2012
Volume
3 - 2012
Edited by
Deborah Mason, Cardiff University, UK
Reviewed by
Elena Adinolfi, University of Ferrara, Italy; Gudrun Stenbeck, Brunel University, UK
Copyright
© Rumney, Wang, Agrawal and Gartland.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
*Correspondence: Alison Gartland, Department of Human Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield, Beech Hill Road, Sheffield, South Yorkshire S10 2RX, UK. e-mail: a.gartland@sheffield.ac.uk
This article was submitted to Frontiers in Bone Research, a specialty of Frontiers in Endocrinology.
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.