ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Diabetes: Molecular Mechanisms
This article is part of the Research TopicGenetic Mechanisms in Diabetes PathogenesisView all 16 articles
Abdominal Versus Femoral Subcutaneous Fat Gene Expression in Non-Obese Insulin Resistant Individuals
Provisionally accepted
Saif Badran1*
Shamma Almuraikhy2
Maha Alser2
Khaled Naja2
Najeha Rizwana Anwardeen2
Mohamed Badie Ahmed3
Salma Jarrar3Ghanem Aljassem4Sara Iskeirjeh5Atalla Hammouda3
Suhail Doi2
Mohamed A. Elrayess2- 1Washington University in St Louis, St. Louis, United States
- 2Qatar University, Doha, Qatar
- 3Hamad Medical Corporation, Doha, Qatar
- 4Hamad Medical Corporation Department of Nursing and Midwifery Education, Doha, Qatar
- 5Flinders University, Adelaide, Australia
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Introduction: Adipose tissue, once viewed primarily as an energy reservoir, is now recognized as an endocrine organ involved in various physiological functions, including energy balance and immune responses. Interestingly, recent insights suggest that not all overweight or obese individuals exhibit insulin resistance, guiding the focus towards depot-specific adipose gene expression and its metabolic implications. This pilot study investigates adipose depot-specific differences in gene expression in abdominal versus femoral subcutaneous adipose tissue (SAT) among non-obese, insulin-sensitive (IS) and insulin-resistant (IR) individuals, aiming to elucidate underlying mechanisms of metabolic dysfunction. Methods: Seventeen non-obese participants scheduled for body contouring surgery provided paired SAT biopsies from abdominal and femoral regions. Based on HOMA-IR scores, participants were categorized into IS and IR groups. Gene expression of key markers including GATA3, PPAR-γ, adiponectin, PGC-1α, TNF-α, and IL-6 were analyzed using real-time PCR and Western Blot. Vitamin D levels were also measured. Results: Our analysis revealed a complex depot-specific regulation of GATA3 expression in human subcutaneous adipose tissue. While GATA3 protein showed a trend toward higher expression in abdominal SAT, its mRNA levels were significantly elevated in thigh SAT exclusively in insulin-resistant individuals. Furthermore, circulating vitamin D levels correlated significantly with GATA3 and PPARγ expression specifically in thigh adipose tissue. Conclusion: This pilot study reveals new evidence that the functional differences between abdominal and thigh subcutaneous adipose tissue also encompass transcriptional and post-transcriptional regulation of key adipogenic factors.
Keywords: Adipose tissue dysfunction, GATA3, Gene Expression, Insulin Resistance, PPAR-γ, subcutaneous adipose tissue
Received: 13 Aug 2025; Accepted: 10 Dec 2025.
Copyright: © 2025 Badran, Almuraikhy, Alser, Naja, Anwardeen, Ahmed, Jarrar, Aljassem, Iskeirjeh, Hammouda, Doi and Elrayess. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Saif Badran
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