Sodium-glucose cotransporter 2 inhibitors—but not insulin— enhance renal branched-chain amino acid catabolism

Moeko SakamotoNao Hasuzawa^*Lixiang WangKyoko TashiroMiyuki KitamuraShimpei IwataNobuhiko WadaSeiji NomuraSawako MoriyamaMizuki GobaruYukihiro InoguchiAyako NagayamaKenji AshidaYoshinori MORIYAMAMasatoshi Nomura

• School of Medicine, Kurume University, Kurume, Japan

Aims/hypothesis: Sodium–glucose cotransporter 2 inhibitors (SGLT2i) confer cardiorenal protection, and recent work implicates enhanced branched-chain amino acid (BCAA) catabolism as a potential mechanism in the heart. Whether SGLT2i also promote renal BCAA catabolism is largely unknown. We hypothesized that SGLT2i enhance renal BCAA catabolism independently of glycemic effects. Methods: We conducted a prospective, single-center, open-label, nonrandomized, controlled clinical study in patients with type 2 diabetes stably treated with insulin, who were assigned to dapagliflozin (5 mg/day with dose-reduced insulin; n=8/9 completed) or insulin dose-up (n=5/8 completed). At 12 weeks, changes in urinary and plasma metabolites and short-chain acylcarnitines related to BCAA catabolism were quantified. To explore mechanisms, 10-week-old db/db mice received luseogliflozin (10 mg/kg/day, p.o.) or insulin glargine (10 U/day, s.c.) for 4 weeks; renal histology, mRNA and protein expression of key enzymes involved in BCAA catabolism, including branched-chain aminotransferase 2 (BCAT2), branched-chain ketoacid dehydrogenase (BCKDH), and BCKD kinase (BCKDK), were assessed. Results: Dapagliflozin treatment induced greater increases in urinary excretion of three BCAA-derived metabolites—3-hydroxypropionic acid, C5-OH carnitine, and 3-hydroxybutyric acid—compared with insulin at comparable glycemic levels. In contrast, C4 carnitine (an earlier metabolite in valine catabolism) rose more with insulin. No corresponding between-group differences were detected in plasma metabolites. In db/db mice, luseogliflozin attenuated glomerular mesangial expansion and tubular epithelial atrophy, and reduced Col1a1 mRNA and TGF-β1 protein, compared with glargine at comparable glycemic levels. Luseogliflozin decreased the phosphorylated (inactive) form of the BCKDH E1α subunit (p-BCKDHA/BCKDHA) and lowered BCKDK protein. mRNA expression of amino acid transporters and BCAT2 expression was unchanged. Conclusions/interpretation: Across complementary human and mouse studies, SGLT2 inhibition was suggested to enhance renal BCAA catabolism compared with insulin at comparable glycemic levels. In humans, increases in urinary BCAA-derived downstream metabolites without corresponding changes in plasma support a kidney-localized metabolic effect. In mice, SGLT2 inhibitor improved renal histopathology, and reduced phosphorylation-mediated inactivation of BCKDH. These findings provide mechanistic, translational evidence that SGLT2i modulate BCAA flux independently of glucose lowering, suggesting BCAA catabolism as a therapeutic axis in diabetic kidney disease. Clinical trial registration: UMIN000052955.