ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Thyroid Endocrinology
Mitoxantrone Hydrochloride Injection guided Sentinel Lymph Node Biopsy in cN0 BRAF V600E-Mutated Papillary Thyroid Carcinoma: A Feasibility Study
Provisionally accepted
- 1Shandong Second Medical University, Weifang, China
- 2Weifang People's Hospital, Weifang, China
- 3Anqiu People's Hospital, Weifang, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Background: The BRAF V600E mutation in papillary thyroid carcinoma (PTC) is linked to aggressive behavior and frequent occult central lymph node metastases (CLNM), posing a surgical dilemma in clinically node-negative (cN0) patients. Sentinel lymph node biopsy (SLNB) could enable selective intervention, but its accuracy depends on tracer performance. We investigated the utility of SLNB using mitoxantrone hydrochloride injection (MHI) as a novel lymphatic tracer to determine its feasibility and diagnostic performance for occult metastases in patients with cN0, BRAF V600E–mutated, unilateral PTC. Methods: 48 patients with cN0, BRAF V600E-mutated unilateral PTC underwent intraoperative SLNB using MHI, followed by ipsilateral lobectomy with isthmusectomy and prophylactic central neck dissection (PCND). The diagnostic accuracy of SLNB was evaluated by comparing its results with the final histological results from PCND serving as the gold standard. Short-term postoperative complications were recorded to assess the safety of the procedure. Results: Sentinel lymph nodes (SLNs) were identified in 46 out of 48 patients (95.8%), with 62 SLNs retrieved, most commonly in the peritracheal region (93.5%). Intraoperative frozen section analysis identified 21 patients with positive SLNs and 25 with negative SLNs. Final pathology confirmed CLNM in 23 patients (47.9%). The sensitivity, specificity, negative predictive value (NPV), and positive predictive value (PPV) of SLNB for detecting CLNM were 91.3%, 100%, 92.6%, and 100%, respectively. Postoperatively, two patients (4.2%) experienced transient vocal cord palsy, with complete resolution within one week. No cases of parathyroid injury or removal were observed, and there were no instances of transient hypoparathyroidism or permanent complications. Conclusion: In patients with cN0, BRAF V600E–mutated PTC, SLNB using mitoxantrone hydrochloride injection is technically feasible and demonstrates high sensitivity and specificity. This approach may help identify individuals with occult central compartment metastases, thereby potentially avoiding unnecessary PCND in those with negative SLNs.
Keywords: Mitoxantrone hydrochloride injection, Sentinel Lymph Node Biopsy, Papillary thyroid carcinoma, BRAF V600E mutation, Central compartment neck dissection
Received: 25 Oct 2025; Accepted: 30 Nov 2025.
Copyright: © 2025 Li, Li, Li, Song, Men and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xiaojuan Men
Yonghui Wang
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.