Abstract
Alzheimer’s disease (AD) is a progressive neurodegenerative disease. Synaptic dysfunction is an integral feature of AD pathophysiology and a significant factor in early cognitive impairment in AD. Microglia, which are intrinsic immune cells in the central nervous system, play important regulatory roles in the process of synapse formation. Microglia can refine synaptic connections through synaptic clearance to ensure accurate synaptic transmission. Synaptic clearance is not only existed during central nervous system development but also aberrantly activated during AD pathology. However, the mechanisms of synaptic clearance in AD remain to be investigated. TREM2 is involved in the synaptic clearance of microglia, acting alone or with other molecules, such as apolipoprotein E (APOE). In addition, C1q is essential for microglia-mediated synaptic clearance. In this review, we systematically summarized the potential mechanisms of microglia involved in synaptic clearance, comprehensively reviewed the role of TREM2 in microglia regulating synaptic clearance and proposed our hypothesis that TREM2 interacts with APOE and C1q to promote synaptic clearance. This review provides new insights into the role of TREM2 regulation in microglia synaptic clearance and provides potential prospects for the treatment of AD.
Introduction
Alzheimer’s disease (AD) is an age-related neurodegenerative disorder characterized by progressive memory loss and cognitive dysfunction (). The typical pathological changes in AD are neuronal plaques and neurofibrillary tangles (). Oligomeric amyloid-beta peptide (Aβ) has been shown to play an important role in cognitive impairment in AD. The primary targets of Aβ are synapses and synapse-related signaling pathways, which damage synaptic plasticity, learning and memory (). Another key component of AD pathogenesis is tau protein hyperphosphorylation to form neurofibrillary tangles (NFTs). NFTs are less susceptible to degradation by proteolytic enzymes and accumulate in neurons, disrupting the normal function of neuronal axons, leading to structural and functional abnormalities of synapses, interfering with synaptic transmission between neurons, and ultimately leading to neurodegeneration (). Thus, synaptic loss and altered synaptic plasticity are the neurobiological basis of cognitive impairment in Alzheimer’s disease, and synaptic damage is a manifestation of AD pathological impairment.
Synapses are the key sites of functional connections and information transfer between neurons, and synaptic plasticity is the cellular and morphological basis of learning and memory (, ). Synaptic plasticity is thought to play key roles in the development of neural circuitry and impairments in synaptic plasticity contribute to several prominent neuropsychiatric disorders, such as AD (). Synapse clearance, also known as synaptic elimination, can eliminate unnecessary synapses and keep the morphological and functional maturation of the remaining synapses, has been thought to be a critical step in neural circuits stability and an essential mechanism underlying synaptic plasticity in the central nervous system (CNS) (). Mounting evidence indicates that microglia plays an important role in synapse clearance ().
Microglia are resident immune cells in the CNS that regulate brain development, maintenance of neuronal networks, and injury repair (). Microglia serve as brain macrophages. They are responsible for the elimination of protein aggregates, redundant synapses, and other particulate and soluble antigens that may endanger the CNS. Microglia functional changes are implicated in brain development and aging, as well as in neurodegeneration (). Studies have shown that normal microglia phagocytose and clear toxic Aβ oligomers, preventing the development of AD (). However, excessive Aβ oligomers can stimulate the complement cascade pathway and induce microglia to excessively phagocytose and clear synapses in a manner similar to normal synaptic pruning, resulting in synaptic loss in AD patients (). Overall, microglia can mediate synapse loss in AD progression. However, the mechanisms of microglial regulation remain to be investigated.
Triggering receptor expressed on myeloid cells 2 (TREM2) is an AD risk gene identified in recent years, and mutations in its coding region significantly increase the risk of AD (, ). TREM2 is mainly expressed by microglia in the nervous system and regulates microglial activation, proliferation, survival, phagocytosis, and other biological functions. Studies have shown that TREM2 is essential for synaptic clearance (), but the specific mechanism by which TREM2 regulates synaptic clearance still needs to be explored.
Apolipoprotein E (APOE), the strongest genetic risk factor for AD, regulates neurodegeneration mainly by modulating microglial activation (). APOE is synthesized and secreted mainly by astrocytes in the activated state, but in AD, the level of APOE expression in plaque-associated microglia is significantly increased and strongly induced in a TREM2-dependent manner (, ). Emerging evidence suggests that APOE binds to TREM2 and APOE are putative ligands for TREM2 (), thus raising the possibility of an APOE-TREM2 interaction may drive the transcriptional phenotype of dysfunctional microglia and modulate AD pathology (). However, whether APOE-TREM2 pathway participate in microglia-induced synaptic clearance needs further investigation.
Recent findings suggest that the classic complement cascade mediates CNS synaptic clearance (, ). The complement proteins C1q and C3 are involved in synaptic clearance by microglia (). During central nervous system development, redundant neuronal synapses express the complement protein C1q, which is the initiator protein of the classic complement cascade and activates CR3 receptors, and microglia are the only cells in the brain that can express CR3 receptors (). Activation of the complement signaling pathway allows microglia to recognize synapses, leading to synaptic clearance (). Inhibition of C1q, C3 or the microglial complement receptor CR3 reduces microglial phagocytosis and prevent synaptic loss ().
In this review, we systematically summarized the possible mechanisms of microglia involved in synaptic clearance in AD, comprehensively reviewed the role of TREM2 in microglia regulating synaptic clearance and explore the relationship of TREM2, APOE and C1q in synaptic clearance of microglia. We proposed our hypothesis that TREM2 interacts with APOE and C1q to promote synaptic clearance and impaired synaptic plasticity in AD. This review provides new insights into the role of TREM2 regulation in microglia synaptic clearance and provides potential prospects for the treatment of AD.
Role of Microglia in Synaptic Clearance
Microglia and Synapses in AD
Neurons in the brain rapidly extend their axons and dendrites after birth and form cellular connections between neurons (). Synapses are the key sites of neuronal information transmission and play an irreplaceable role in the CNS (). Synaptic loss is one of the important histological changes in AD patients, and the degree of synaptic loss correlates with the degree of dementia in AD patients (–).
Recent evidence shows that microglia can create tight connections with neuronal synapses in vivo and continually contact synapses in an activity and experience dependent manner, and play a functionally dynamic role in synaptic plasticity (). Microglia can also contribute to structural plasticity through the elimination of synapses via phagocytic mechanisms, which is necessary for normal cognition (). Based on these evidence, we suggest that microglia is essential for normal synaptic and structural plasticity that supports cognition (). While in AD condition, plenty of evidence show microglial interactions with synapses obviously affect the maturation of synapses and neuronal viability (, ). For example, previous studies have shown that the accumulation of soluble Aβ activates microglia in the brain to release proinflammatory factors that mediate synaptic dysfunction (). Activated microglia also clear synapses and promote synaptic remodeling ().
In a word, synaptic dysfunction is a key initiator of AD pathology. Elucidating the underlying molecular mechanisms of microglia-synapse pathways may provide further insights into immune system regulation of neuronal circuit development in the AD brain and novel approaches for AD treatment.
Microglia-Mediated Synaptic Clearance
In the last few years, plenty studies have shown that microglia can mediate synaptic clearance. The pathway of complement-mediated synaptic clearance has been extensively studied (). Synapses requiring pruning can be localized and labelled by classic complement component 1q (C1q) and phagocytosed by complement receptor 3 (CR3)-mediated microglia (, ). Functional blockade of C1q by C1q antibodies or artificial knockdown of the C1q gene can reduce Aβ oligomer-induced and tau-induced excessive synaptic phagocytosis and improve synaptic deficits (, ). In addition, removal of the complement component C3 or knockdown of the CR3 gene also protected synaptic loss in the brains of AD transgenic mice (, ).
Besides complement system, TREM2 is also essential for microglia-mediated synaptic pruning (). A lack of TREM2 receptors leads to impaired synaptic clearance, increased dendritic spine density and enhanced excitatory neurotransmission (). Studies have shown that phosphatidylserine exposure at the synapse may be an “eat me” signal for TREM2 receptors (). Therefore, TREM2 can mediate synaptic clearance by regulating microglia phagocytosis.
Another pathway through which microglia mediate synaptic clearance is the phagocytic signaling pathway of C-X3-C motile chemokine receptor 1 (CX3CR1) (). In mice lacking Cx3cr1, microglia numbers were transiently reduced in the developing brain and synaptic pruning was delayed (). Knockdown of CX3CL1 resulted in reduced social interactions and increased repetitive behavioral traits in both juvenile and adult mice (). These results strongly suggest that microglia perform synaptic clearance via CX3CL1/CX3CR1 signaling.
In AD pathology, the abnormal mechanism of synaptic clearance mediated by microglia is not clear. Hence, we will summarize the various factors associated with microglia-mediated synaptic clearance in AD.
The Mechanism May Involve in Microglia-Mediated Synaptic Clearance in AD
In recent years, genome-wide association studies (GWAS) have identified more than 25 genetic loci that are strongly associated with the risk of developing late-onset Alzheimer’s disease (LOAD), one of them is TREM2 gene variants (). TREM2 has been suggested to play a crucial role in AD pathogenesis. TREM2 may act as a multifaceted player in microglial functions in AD brain homeostasis. TREM2 can not only influence microglial functions in amyloid and taupathologies, but also participate in inflammatory responses and metabolism. In this review, we will focus the role of TREM2 in microglia-mediated synaptic clearance in AD.
APOE is also undoubtedly associated with LOAD (). AD patients carrying the ϵ4 allele of APOE have an earlier age of onset, more severe amyloid, tau deposition and brain atrophy, and more rapid disease progression (). APOE not only involve in the aggregation and clearance of Aβ but also participate in the regulation of microglial activation, immune regulation and cytokine release (). However, the role of APOE in synaptic clearance and neural pathway regulation needs to be further explored.
Furthermore, the complement system has recently been shown to plays both neuroprotective and neuroinflammatory roles in AD pathophysiology (). During the early stages of AD, the complement system can succeed in clearing Aβ in conjunction with glial cells (). However, when Aβ starts to accumulate and Aβ plaques develop, the complement system can further increase the phagocytic capability of microglia (). Here, we will discuss how the complement system is involved in the effective functioning of microglia-mediated synaptic clearance in Alzheimer’s disease.
TREM2 Is Involved in Synaptic Clearance by Microglia in AD
TREM2 is a transmembrane protein expressed in myeloid microglia and play important roles in functional maintenance of normal brain activity (). Recent whole-genome sequencing studies have shown that rare TREM2 variants increase the risk of AD by 2-4-fold (). In the cerebrospinal fluid (CSF) of AD patients, soluble TREM2 has been shown to correlate with total tau and phosphorylated tau (p-tau, Thr181) levels (). Therefore, TREM2 has become a new hotspot in the study of AD pathogenesis and treatment.
TREM2 regulates a variety of biological functions (–), but the molecular mechanisms involved in AD pathogenesis are unclear. Most of the current studies on TREM2 regulation of microglial function have focused on its role in the regulation of abnormal protein accumulation and neuroinflammation (–). For example, TREM2 has been shown to have a critical role in reducing Aβ-induced neuronal atrophy and tau seeding/spreading (, , ). Other studies also point out TREM2 may affect the inflammatory response and energy metabolism, regulating microglial phagocytosis of abnormal proteins and damaged neurons (, ).
It is worth mentioning that several recent studies suggest that TREM2 is also essential for synaptic clearance by microglia (, , ). In vitro experiments showed that TREM2 knockdown significantly affected microglial activation and phagocytosis. In vivo, TREM2 mutant mice showed abnormal microglial synaptic phagocytosis, and functional magnetic resonance imaging also revealed altered functional connectivity in neural pathways throughout the brain (). In TREM2-deficient mice, microglia fail to recognize redundant synapses, synaptic modification is absent, neuronal synaptic density is increased, arousal is enhanced, normal brain function is impaired, and the mice develop abnormal behaviors and social deficits (). All in all, these data suggest microglia modified synapses via phagocytosis, which required the involvement of TREM2.
APOE Interacts With TREM2 in AD
Both APOE and TREM2 were identified as independent risk factors for LOAD. They are also expressed in glia cells and related immune response. Both TREM2 and APOE play important roles in promoting microglial activation, survival, and barrier formation. TREM2 expression and function are positively associated with APOE expression in AD pathology (), and APOE expression may be dependent on TREM2 regulation (). Several groups have shown that TREM2 binds to APOE (–). APOE can increase the phagocytosis of apoptotic neurons via the TREM2 pathway. The TREM2 R47H variant was shown to reduce TREM2 affinity to bind APOE. Besides, recent study suggests that human APOE contain the binding site for TREM2, and that there is an APOE-isoform-dependent binding to TREM2. Recent reports also identified TREM2-APOE pathway can drive the transcriptional phenotype of dysfunctional microglia in AD, which can induce a shift from a homeostatic phenotype to a neurodegenerative phenotype in microglia ().
Overall, emerging evidence suggests that APOE binds to TREM2 and APOE are putative ligands for TREM2, thus raising the possibility of an APOE-TREM2 interaction modulating different aspects of AD pathology. TREM2-APOE interaction may promote microglial activation, survival, phagocytosis and thus modulate inflammatory responses and neuronal injury. However, whether the TREM2-APOE pathway can modulate microglial synaptic clearance remains unclear and needs to be further investigated.
Complement Signaling Pathway Involves in Microglia-Mediated Synaptic Clearance
Previous studies have identified the complement signaling pathway as a component of the innate immune system that is involved in synaptic clearance by microglia in AD (, ). C1q, the initiating protein in the classical complement cascade, is expressed by postnatal neurons in response to immature astrocytes and is localized at synapses (). During CNS development, redundant neuronal synapses express the complement protein C1q, which activates CR3 receptors, and since microglia are the only cells that can express CR3 in the brain, activation of the complement signaling pathway enables the recognition of synapses by microglia, leading to phagocytosis and synaptic clearance. Mice lacking either complement protein C1q or downstream complement protein C3 exhibit a greater sustained deficit in CNS synaptic elimination (, ). Hong et al. examined a mouse model of Alzheimer’s disease and showed that C1q was significantly increased before significant plaque deposition and was associated with synapse clearance (). Inhibition of C1q, C3, or the complement receptor CR3 in microglia reduces the number of phagocytic microglia and decreases the extent of synaptic loss in AD.
C1q and APOE were previously thought to act separately and perform independent tasks in many tissue contexts. Recent studies have shown a correlation between C1q and APOE (). Currently, C1q has been shown to colocalize with APOE in the brain in human AD (). In vitro experiments also demonstrated that APOE is a checkpoint inhibitor of classic complement cascade activity (). All human APOE isoforms attenuate classic complement cascade activity in vitro through high affinity binding to activated C1q. The C1q-APOE complex is a marker of sustained complement activity in Aβ plaques in vivo. The C1q-APOE complex in Aβ plaques is associated with reduced cognitive performance. APOE activates C1q through the formation of the C1q-APOE complex, which in turn initiates the classic complement cascade. In AD, Aβ deposits may lead to the loss of synapses by activating complement-related clearance pathways. In summary, APOE binds to C1q and regulates the activation of the classic complement cascade. C1q-APOE complex may affect AD by regulating synaptic clearance by microglia.
Moreover, in recent years, it has been found that the levels of both TREM2 and C1q are significantly increased around Aβ, suggesting an association between TREM2 and C1q in microglia-mediated synaptic phagocytosis (, ). Therefore, clarifying the interaction between TREM2 and C1q in microglia-mediated synaptic clearance will further refine our understanding of TREM2 and the complement system in AD.
In conclusion, the exact relationship between TREM2, APOE and C1q is still unclear. Therefore, further in-depth and comprehensive studies are needed to investigate how these three pathways regulate microglial synaptic clearance in AD Table 1.
Table 1
| Factors | Mechanism in synaptic clearance in Alzheimer’s Disease |
|---|---|
| TREM2 | 1) Lack of TREM2 receptors lead to impaired synaptic clearance. 2)TREM2 knockdown significantly affect microglial activation and phagocytosis. 3) TREM2 recognize phosphatidylserine “eat me” signal at the synapses. |
| C1q | 1) Synapses requiring pruning can be localized and labelled by C1q. 2) Inhibition of C1q can reduce Aβ oligomer-induced and tau-induced excessive synaptic phagocytosis and improve synaptic deficits. 3) Removal of the complement component protect synaptic loss in the brains of AD transgenic mice. |
| CX3CR1 | 1) In mice lacking Cx3cr1, synaptic pruning is delayed. 2) Knockdown of CX3CL1 results in reduced social interactions and increases repetitive behavioral traits. |
| APOE | 1) APOE can increase the phagocytosis of apoptotic neurons via the TREM2 pathway. 2) APOE is a checkpoint inhibitor of classic complement cascade activity. |
| TREM2-APOE | 1) APOE binds to TREM2 and APOE are putative ligands for TREM2 2) TREM2-APOE pathway can drive the transcriptional phenotype of dysfunctional microglia in AD. 3) TREM2-APOE interaction promotes microglial activation, survival, phagocytosis. |
| APOE-C1q | 1) C1q has been shown to colocalize with APOE in the brain in human AD. 2) APOE activates C1q through the formation of the C1q-APOE complex, which in turn initiates the classic complement cascade. 3) C1q-APOE complex affect microglia-mediated synaptic clearance. |
| TREM2-C1q | An association between TREM2 and C1q in microglia-mediated synaptic phagocytosis needs to be explored. |
The specific mechanism in microglia-mediated synaptic clearance in Alzheimer’s Disease.
Summary and Prospects
As the most important immune cells in the brain, microglia play significant roles in almost all central nervous system disease processes. The studies of microglia function have been a hot topic in the field of AD research. Microglia can refine synaptic connections through phagocytosis to ensure accurate transmission of information. TREM2, a microglia surface receptor, is essential for synaptic clearance by microglia, but the exact mechanism needs to be further explored. TREM2 binds with high affinity to APOE and promotes the clearance of apoptotic neurons and cellular debris by microglia. Previous studies have identified the complement signaling pathway is also involved in synaptic clearance. APOE may affect the classic complement cascade through the C1q-APOE complex, which affects microglia and influences synaptic pruning. In recent years, significantly increased levels of both TREM2 and the complement protein C1q have been found around Aβ, suggesting an association between TREM2 and C1q. Therefore, we propose that TREM2, APOE and C1q interact with each other and promote microglia mediated synaptic clearance in AD Figure 1.
Figure 1
In this review, we systematically summarized the potential mechanisms of microglia involved in synaptic clearance, comprehensively reviewed the role of TREM2 in microglia regulating synaptic clearance and proposed our hypothesis that TREM2 interacts with APOE and C1q to promote synaptic clearance in AD. This review provides new insights into the role of TREM2 regulation in microglia synaptic clearance and provides new clues to elucidate the role of TREM2 in the pathogenesis of AD.
Funding
This work was supported by Capital’s Funds for HealthImprovement and Research [grant number CFH 2020-4-1033]; Beijing NOVA Program [grant number Z211100002121051];Beijing Hospitals Authority Innovation Studio of Young Staff Funding Support [grant number 202118] and Young Elite Scientists Sponsorship Program by CAST [grant number 2021QNRC001].
Publisher’s Note
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Statements
Author contributions
QQ: Conceptualization, Writing - review and editing, Supervision. MW: Conceptualization, Writing - original draft, Writing - review and editing. YY: Writing - original draft. YT: Conceptualization, Writing - review and editing, Supervision, Project administration. All authors read and approved the final manuscript.
Acknowledgments
We thank Prof. Zhaoqian Teng of the Institute of Stem Cell and Regeneration, Chinese Academy of Sciences for revising the article. The authors thank nature research editing (https://authorservices.springernature.com) for editorial assistance.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Abbreviations
AD, Alzheimer’s disease; APOE, apolipoprotein E; Aβ, amyloid-β; C1q:complement component 1q; CNS, central nervous system; CR3:complement receptor 3; CSF, cerebrospinal fluid; GWAS, Genome-wide association studies; LOAD, late-onset AD; NFTs, neurofibrillary tangles; TREM2, triggering receptor expressed on myeloid cells 2.
References
1
BarnettR. Alzheimer's Disease. Lancet (2019) 393(10181):1589. doi: 10.1016/S0140-6736(19)30851-7
2
ReitzCBrayneCMayeuxR. Epidemiology of Alzheimer Disease. Nat Rev Neurol (2011) 7(3):137–52. doi: 10.1038/nrneurol.2011.2
3
TuSOkamotoSLiptonSAXuH. Oligomeric Abeta-Induced Synaptic Dysfunction in Alzheimer's Disease. Mol Neurodegener (2014) 9:48. doi: 10.1186/1750-1326-9-48
4
PiccioniGMangoDSaidiACorboMNisticòR. Targeting Microglia-Synapse Interactions in Alzheimer's Disease. Int J Mol Sci (2021) 22(5):2342. doi: 10.3390/ijms22052342
5
SüdhofTC. Towards an Understanding of Synapse Formation. Neuron (2018) 100(2):276–93. doi: 10.1016/j.neuron.2018.09.040
6
MartinSJGrimwoodPDMorrisRG. Synaptic Plasticity and Memory: An Evaluation of the Hypothesis. Annu Rev Neurosci (2000) 23:649–711. doi: 10.1146/annurev.neuro.23.1.649
7
CitriAMalenkaRC. Synaptic Plasticity: Multiple Forms, Functions, and Mechanisms. Neuropsychopharmacology (2008) 33(1):18–41. doi: 10.1038/sj.npp.1301559
8
CardozoPLde LimaIBQMacielEMASilvaNCDobranskyTRibeiroFM. Synaptic Elimination in Neurological Disorders. Curr Neuropharmacol (2019) 17(11):1071–95. doi: 10.2174/1570159X17666190603170511
9
RajendranLPaolicelliRC. Microglia-Mediated Synapse Loss in Alzheimer's Disease. J Neurosci (2018) 38(12):2911–9. doi: 10.1523/JNEUROSCI.1136-17.2017
10
Augusto-OliveiraMArrifanoGPLopes-AraujoASantos-SacramentoLTakedaPYAnthonyDCet al. What Do Microglia Really Do in Healthy Adult Brain? Cells (2019) 8(10):1293. doi: 10.3390/cells8101293
11
ColonnaMButovskyO. Microglia Function in the Central Nervous System During Health and Neurodegeneration. Annu Rev Immunol (2017) 35:441–68. doi: 10.1146/annurev-immunol-051116-052358
12
HemonnotALHuaJUlmannLHirbecH. Microglia in Alzheimer Disease: Well-Known Targets and New Opportunities. Front Aging Neurosci (2019) 11:233. doi: 10.3389/fnagi.2019.00233
13
HansenDVHansonJE. Microglia in Alzheimer's Disease. J Cell Biol (2018) 217(2):459–72. doi: 10.1083/jcb.201709069
14
QinQTengZLiuCLiQYinYTangY. TREM2, Microglia, and Alzheimer's Disease. Mech Ageing Dev (2021) 195:111438. doi: 10.1016/j.mad.2021.111438
15
JonssonTStefanssonHSteinbergSJonsdottirIJonssonPVSnaedalJet al. Variant of TREM2 Associated With the Risk of Alzheimer's Disease. N Engl J Med (2013) 368(2):107–16. doi: 10.1056/NEJMoa1211103
16
FilipelloFMoriniRCorradiniIZerbiVCanziAMichalskiBet al. The Microglial Innate Immune Receptor TREM2 Is Required for Synapse Elimination and Normal Brain Connectivity. Immunity (2018) 48(5):979–91.e8. doi: 10.1016/j.immuni.2018.04.016
17
YamazakiYZhaoNCaulfieldTRLiuCCBuG. Apolipoprotein E and Alzheimer Disease: Pathobiology and Targeting Strategies. Nat Rev Neurol (2019) 15(9):501–18. doi: 10.1038/s41582-019-0228-7
18
NguyenATWangKHuGWangXMiaoZAzevedoJAet al. APOE and TREM2 Regulate Amyloid-Responsive Microglia in Alzheimer's Disease. Acta Neuropathol (2020) 140(4):477–93. doi: 10.1007/s00401-020-02200-3
19
AtagiYLiuCCPainterMMChenXFVerbeeckCZhengHet al. Apolipoprotein E Is a Ligand for Triggering Receptor Expressed on Myeloid Cells 2 (Trem2). J Biol Chem (2015) 290(43):26043–50. doi: 10.1074/jbc.M115.679043
20
WolfeCMFitzNFNamKNLefterovIKoldamovaR. The Role of APOE and TREM2 in Alzheimer's Disease-Current Understanding and Perspectives. Int J Mol Sci (2018) 20(1):81. doi: 10.3390/ijms20010081
21
PresumeyJBialasARCarrollMC. Complement System in Neural Synapse Elimination in Development and Disease. Adv Immunol (2017) 135:53. doi: 10.1016/bs.ai.2017.06.004
22
StevensBAllenNJVazquezLEHowellGRChristophersonKSNouriNet al. The Classical Complement Cascade Mediates CNS Synapse Elimination. Cell -CAMBRIDGE MA- (2007) 131(6):1164–78. doi: 10.1016/j.cell.2007.10.036
23
RicklinDReisESLambrisJD. Complement in Disease: A Defence System Turning Offensive. Nat Rev Nephrol (2016) 12(7):383–401. doi: 10.1038/nrneph.2016.70
24
StephanAHBarresBAStevensB. The Complement System: An Unexpected Role in Synaptic Pruning During Development and Disease. Annu Rev Neurosci (2012) 35(1):369–89. doi: 10.1146/annurev-neuro-061010-113810
25
DalakasMCAlexopoulosHSpaethPJ. Complement in Neurological Disorders and Emerging Complement-Targeted Therapeutics. Nat Rev Neurol (2020) 16(11):601–17. doi: 10.1038/s41582-020-0400-0
26
WerneburgSJungJKunjammaRBHaSKLucianoNJWillisCMet al. Targeted Complement Inhibition at Synapses Prevents Microglial Synaptic Engulfment and Synapse Loss in Demyelinating Disease. Immunity (2020) 52(1):167–82.e7. doi: 10.1016/j.immuni.2019.12.004
27
SudhofTC. The Cell Biology of Synapse Formation. J Cell Biol (2021) 220(7):e202103052. doi: 10.1083/jcb.202103052
28
Cuestas TorresDMCardenasFP. Synaptic Plasticity in Alzheimer's Disease and Healthy Aging. Rev Neurosci (2020) 31(3):245–68. doi: 10.1515/revneuro-2019-0058
29
FornerSBaglietto-VargasDMartiniACTrujillo-EstradaLLaFerlaFM. Synaptic Impairment in Alzheimer's Disease: A Dysregulated Symphony. Trends Neurosci (2017) 40(6):347–57. doi: 10.1016/j.tins.2017.04.002
30
RobinsonJLMolina-PorcelLCorradaMMRaibleKLeeEBLeeVMet al. Perforant Path Synaptic Loss Correlates With Cognitive Impairment and Alzheimer's Disease in the Oldest-Old. Brain (2014) 137(Pt 9):2578–87. doi: 10.1093/brain/awu190
31
MorrisGPClarkIAZinnRVisselB. Microglia: A New Frontier for Synaptic Plasticity, Learning and Memory, and Neurodegenerative Disease Research. Neurobiol Learn Mem (2013) 105:40–53. doi: 10.1016/j.nlm.2013.07.002
32
WuYDissing-OlesenLMacVicarBAStevensB. Microglia: Dynamic Mediators of Synapse Development and Plasticity. Trends Immunol (2015) 36(10):605–13. doi: 10.1016/j.it.2015.08.008
33
EikelenboomPVeerhuisR. The Role of Complement and Activated Microglia in the Pathogenesis of Alzheimer's Disease. Neurobiol Aging (1996) 17(5):673–80. doi: 10.1016/0197-4580(96)00108-X
34
FuHLiuBFrostJLHongSJinMOstaszewskiBet al. Complement Component C3 and Complement Receptor Type 3 Contribute to the Phagocytosis and Clearance of Fibrillar Abeta by Microglia. Glia (2012) 60(6):993–1003. doi: 10.1002/glia.22331
35
FonsecaMIZhouJBottoMTennerAJ. Absence of C1q Leads to Less Neuropathology in Transgenic Mouse Models of Alzheimer's Disease. J Neurosci (2004) 24(29):6457–65. doi: 10.1523/JNEUROSCI.0901-04.2004
36
WebsterSDGalvanMFerranEGarzon-RodriguezWTennerAJ. Antibody-Mediated Phagocytosis of the Amyloid -Peptide in Microglia Is Differentially Modulated by C1q. J Immunol (2001) 166(12):7496–503. doi: 10.4049/jimmunol.166.12.7496
37
DejanovicBHuntleyMADe MaziereAMeilandtWJWuTSrinivasanKet al. Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q Antibodies. Neuron (2018) 100(6):1322–36.e7. doi: 10.1016/j.neuron.2018.10.014
38
ShiQChowdhurySMaRLeKXHongSCaldaroneBJet al. Complement C3 Deficiency Protects Against Neurodegeneration in Aged Plaque-Rich APP/PS1 Mice. Sci Transl Med (2017) 9(392):eaaf6295. doi: 10.1126/scitranslmed.aaf6295
39
QuWLiL. Loss of TREM2 Confers Resilience to Synaptic and Cognitive Impairment in Aged Mice. J Neurosci (2020) 40(50):9552–63. doi: 10.1523/JNEUROSCI.2193-20.2020
40
Scott-HewittNPerrucciFMoriniRErreniMMahoneyMWitkowskaAet al. Local Externalization of Phosphatidylserine Mediates Developmental Synaptic Pruning by Microglia. EMBO J (2020) 39(16):e105380. doi: 10.15252/embj.2020105380
41
MeccaCGiambancoIDonatoRArcuriC. Microglia and Aging: The Role of the TREM2–DAP12 and CX3CL1-CX3CR1 Axes. Int J Mol Sci (2018) 19(1):318. doi: 10.3390/ijms19010318
42
PaolicelliRCBolascoGPaganiFMaggiLScianniMPanzanelliPet al. Synaptic Pruning by Microglia Is Necessary for Normal Brain Development. Science (2011) 333(6048):1456–8. doi: 10.1126/science.1202529
43
RogersJTMorgantiJMBachstetterADHudsonCEPetersMMGrimmigBAet al. CX3CR1 Deficiency Leads to Impairment of Hippocampal Cognitive Function and Synaptic Plasticity. J Neurosci (2011) 31(45):16241–50. doi: 10.1523/JNEUROSCI.3667-11.2011
44
KimJH. Genetics of Alzheimer's Disease. Dement Neurocogn Disord (2018) 17(4):131–6. doi: 10.12779/dnd.2018.17.4.131
45
HoltzmanDMHerzJBuG. Apolipoprotein E and Apolipoprotein E Receptors: Normal Biology and Roles in Alzheimer Disease. Cold Spring Harbor Perspect Med (2012) 2(3):a006312. doi: 10.1101/cshperspect.a006312
46
LanfrancoMFNgCARebeckGW. ApoE Lipidation as a Therapeutic Target in Alzheimer's Disease. Int J Mol Sci (2020) 21(17):6336. doi: 10.3390/ijms21176336
47
ShahAKishoreUShastriA. Complement System in Alzheimer's Disease. Int J Mol Sci (2021) 22(24):13647. doi: 10.3390/ijms222413647
48
KolevMVRusevaMMHarrisCLMorganBPDonevRM. Implication of Complement System and Its Regulators in Alzheimer's Disease. Curr Neuropharmacol (2009) 7(1):1–8. doi: 10.2174/157015909787602805
49
PasinettiGM. Inflammatory Mechanisms in Neurodegeneration and Alzheimer's Disease: The Role of the Complement System. Neurobiol Aging (1996) 17(5):707–16. doi: 10.1016/0197-4580(96)00113-3
50
HickmanSEEl KhouryJ. TREM2 and the Neuroimmunology of Alzheimer's Disease. Biochem Pharmacol (2014) 88(4):495–8. doi: 10.1016/j.bcp.2013.11.021
51
Suárez-CalvetMKleinbergerGCaballeroMABrendelMRomingerAAlcoleaDet al. Strem2 Cerebrospinal Fluid Levels Are a Potential Biomarker for Microglia Activity in Early-Stage Alzheimer's Disease and Associate With Neuronal Injury Markers. EMBO Mol Med (2016) 8(5):466–76. doi: 10.15252/emmm.201506123
52
JayTRMillerCMChengPJGrahamLCBemillerSBroihierMLet al. TREM2 Deficiency Eliminates TREM2+ Inflammatory Macrophages and Ameliorates Pathology in Alzheimer's Disease Mouse Models. J Exp Med (2015) 212(3):287–95. doi: 10.1084/jem.20142322
53
UllandTKSongWMHuangSCUlrichJDSergushichevABeattyWLet al. TREM2 Maintains Microglial Metabolic Fitness in Alzheimer's Disease. Cell (2017) 170(4):649–63.e13. doi: 10.1016/j.cell.2017.07.023
54
YuanPCondelloCKeeneCDWangYBirdTPaulSet al. TREM2 Haplodeficiency in Mice and Humans Impairs the Microglia Barrier Function Leading to Decreased Amyloid Compaction and Severe Axonal Dystrophy. Neuron (2016) 90(4):724–39. doi: 10.1016/j.neuron.2016.05.003
55
LeynsCEGUlrichJDFinnMBStewartFRKoscalLJRemolina SerranoJet al. TREM2 Deficiency Attenuates Neuroinflammation and Protects Against Neurodegeneration in a Mouse Model of Tauopathy. Proc Natl Acad Sci U S A (2017) 114(43):11524–9. doi: 10.1073/pnas.1710311114
56
BemillerSMMccrayTJAllanKFormicaSVXuGWilsonGet al. TREM2 Deficiency Exacerbates Tau Pathology Through Dysregulated Kinase Signaling in a Mouse Model of Tauopathy. Mol Neurodegeneration (2017) 12(1):74. doi: 10.1186/s13024-017-0216-6
57
LiJTZhangY. TREM2 Regulates Innate Immunity in Alzheimer's Disease. JÂ Neuroinflammation (2018) 15(1):107. doi: 10.1186/s12974-018-1148-y
58
LeeSHMeilandtWJXieLGandhamVDHansenDV. Trem2 Restrains the Enhancement of Tau Accumulation and Neurodegeneration by β-Amyloid Pathology. Neuron (2021) 109(8):1283–301. doi: 10.1016/j.neuron.2021.02.010
59
ShengLChenMCaiKSongYYuDZhangHet al. Microglial Trem2 Induces Synaptic Impairment at Early Stage and Prevents Amyloidosis at Late Stage in APP/PS1 Mice. FASEB J (2019) 33(9):10425–42. doi: 10.1096/fj.201900527R
60
UlrichJDFinnMBWangYShenAMahanTEHongJet al. Altered Microglial Response to Aβ Plaques in APPPS1-21 Mice Heterozygous for TREM2. Mol Neurodegeneration (2014) 3:9:20. doi: 10.1186/1750-1326-9-20
61
YehFLWangYTomIGonzalezLCShengM. TREM2 Binds to Apolipoproteins, Including APOE and CLU/APOJ, and Thereby Facilitates Uptake of Amyloid-Beta by Microglia. Neuron (2016) 91(2):328–40. doi: 10.1016/j.neuron.2016.06.015
62
KrasemannSMadoreCCialicRBaufeldCCalcagnoNEl FatimyRet al. The TREM2-APOE Pathway Drives the Transcriptional Phenotype of Dysfunctional Microglia in Neurodegenerative Diseases. Immunity (2017) 47(3):566–81.e9. doi: 10.1016/j.immuni.2017.08.008
63
BaileyCCDeVauxLBFarzanM. The Triggering Receptor Expressed on Myeloid Cells 2 Binds Apolipoprotein E. J Biol Chem (2015) 290(43):26033–42. doi: 10.1074/jbc.M115.677286
64
FraserDAPisalyaputKTennerAJ. C1q Enhances Microglial Clearance of Apoptotic Neurons and Neuronal Blebs, and Modulates Subsequent Inflammatory Cytokine Production. J Neurochem (2010) 112(3):733–43. doi: 10.1111/j.1471-4159.2009.06494.x
65
HongSBeja-GlasserVFNfonoyimBMFrouinAStevensB. Complement and Microglia Mediate Early Synapse Loss in Alzheimer Mouse Models. Science (2016) 352(6286):712. doi: 10.1126/science.aad8373
66
VogtLMKwasniewiczETalensSScaveniusCBieleckaEEkdahlKNet al. Apolipoprotein E Triggers Complement Activation in Joint Synovial Fluid of Rheumatoid Arthritis Patients by Binding C1q. J Immunol (2020) 204(10):2779–90. doi: 10.4049/jimmunol.1900372
67
ThangavelRBhagavanSMRamaswamySBSurpurSGovindarajanRKempurajDet al. Co-Expression of Glia Maturation Factor and Apolipoprotein E4 in Alzheimer's Disease Brain. J Alzheimers Dis (2017) 61(2):553–60. doi: 10.3233/JAD-170777
68
YinCAckermannSMaZMohantaSKZhangCLiYet al. ApoE Attenuates Unresolvable Inflammation by Complex Formation With Activated C1q. Nat Med (2019) 25(3):496–506. doi: 10.1038/s41591-018-0336-8
Summary
Keywords
Alzheimer disease, synaptic clearance, microglia, TREM2 (triggering receptor expressed on myeloid cells), APOE, complement
Citation
Qin Q, Wang M, Yin Y and Tang Y (2022) The Specific Mechanism of TREM2 Regulation of Synaptic Clearance in Alzheimer’s Disease. Front. Immunol. 13:845897. doi: 10.3389/fimmu.2022.845897
Received
30 December 2021
Accepted
19 April 2022
Published
19 May 2022
Volume
13 - 2022
Edited by
Jorge Matias-Guiu, Complutense University of Madrid, Spain
Reviewed by
Marta Olah, Columbia University Irving Medical Center, United States; Meerambika Mishra, Sambalpur University, India
Updates
Copyright
© 2022 Qin, Wang, Yin and Tang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Yi Tang, tangyi@xwhosp.org
†These authors have contributed equally to this work
This article was submitted to Multiple Sclerosis and Neuroimmunology, a section of the journal Frontiers in Immunology
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