Abstract
Macrophages are the preeminent phagocytic cells which control multiple infections. Tuberculosis a leading cause of death in mankind and the causative organism Mycobacterium tuberculosis (MTB) infects and persists in macrophages. Macrophages use reactive oxygen and nitrogen species (ROS/RNS) and autophagy to kill and degrade microbes including MTB. Glucose metabolism regulates the macrophage-mediated antimicrobial mechanisms. Whereas glucose is essential for the growth of cells in immune cells, glucose metabolism and its downsteam metabolic pathways generate key mediators which are essential co-substrates for post-translational modifications of histone proteins, which in turn, epigenetically regulate gene expression. Herein, we describe the role of sirtuins which are NAD+-dependent histone histone/protein deacetylases during the epigenetic regulation of autophagy, the production of ROS/RNS, acetyl-CoA, NAD+, and S-adenosine methionine (SAM), and illustrate the cross-talk between immunometabolism and epigenetics on macrophage activation. We highlight sirtuins as emerging therapeutic targets for modifying immunometabolism to alter macrophage phenotype and antimicrobial function.
Introduction
Macrophages are the preeminent phagocytic cells which respond to pathogen invasion using a variety of anti-microbial mechanisms. Circulating monocytes originating in bone marrow become macrophages (MΦs) at tissue sites of infection after getting exposed to cytokines and microbial stimuli. During tuberculosis, the causative organism Mycobacterium tuberculosis (MTB) infects and grows in naive MΦs. That tuberculosis continues to kill more than a million people each year indicates that MTB has evasion mechanisms to survive and grow in MΦs. Indeed, MTB evades antimicrobial mechanisms of MΦs using multiple strategies including epigenetic modifications (1, 2). For example, MTB encodes for dozens of methyltransferases of which, products from Rv1988 and Rv2966c methylate DNA (2, 3); DNA hypermethylation of MΦs was reported to decrease immunity in TB patients (4). MTB derived Rv3423.1 acetylates histones affecting gene expression, whereas Enhanced intracellular survival (Eis) protein acetylates histone H3 at K9 and K14 and increases IL-10 (5). Together, these observations suggest that ‘acetylation and methylation’ are important for controlling antimicrobial mechanisms within MΦs during intracellular infections like tuberculosis.
Intriguingly, T cell derived cytokines like IFN-γ drive naïve MΦs into an M1-MΦ phenotype whereas, IL-4, IL-10 and IL-13 differentiate them into M2-MΦs. We recently reported that MTB infected human M1- and M2-MΦs show unique transcriptional responses and M1-MΦs were able to inhibit the growth of MTB using a nitric oxide- and autophagy-dependent mechanism, whereas M2-MΦs were permissive for growth (6). During these studies, we noted that M1- and M2-MΦs expressed differing levels of sirtuin (SIRT) histone/protein deacetylases and significantly, SIRT2 blockade increased the ability of MΦs to kill MTB suggesting a pivotal role.
Recent studies demonstrate that the activity of MΦ derived histone acetyltransferases is regulated by their co-substrate, acetyl-CoA (ac-CoA), whereas the activity of sirtuin proteins which are nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylases, is dependent on NAD+. It is also known that the activity of histone methyltransferases and DNA methyltransferases is regulated by their specific co-substrate, s-adenosylmethionine (SAM) (7). Therefore, chromatin-modifying enzymes can sense the metabolic status and translate this information into gene expression. In MФs, this would determine their polarization state as either pro-inflammatory IFN-γ/LPS inducible M1-MΦs or alternatively activated and anti-inflammatory, IL-4/IL-10 and IL-13 driven M2-MΦs. Interestingly, Glucose metabolism differs between M1- and M2-MΦs and glycolysis and its associated pentose-phosphate- pathway (PPP), serine biosynthesis, and one-carbon metabolism are major sources for the co-substrates for methylation and acetylation. In M1-MΦs, glucose uptake is elevated by up-regulated glucose transporter GLUT1, followed by up-regulated glycolysis (8, 9). High glucose intake and metabolism is essential for phagocytosis, production of reactive-oxygen-species (ROS) and reactive-nitrogen-species (RNS), and secretion of pro-inflammatory cytokines (10).
Emerging evidence also links glycolysis to epigenetics. Locasale’s and Schultz’s groups have demonstrated that histone acetylation is enhanced by glucose flux in a variety of cell types (11, 12). Acetylation is strongly associated with ac-CoA levels but inversely correlated with the ratio of ac-CoA to free CoA (11). Inhibition of glycolysis results in the reduced production of ac-CoA and reduction of histone acetylation leading to differentiation of embryonic stem cells (13). In bacteria, two-thirds of glycolytic and TCA cycle enzymes are acetylated, with acetylation inhibiting their catalytic activity and promoting degradation (14). Glycolysis also regulates histone deacetylation because NAD+-dependent srtuin proteins regulate the expression of glycolytic enzymes and the ratio of NAD+/NADH is controlled by the glycolytic flux, and vice versa (15–18). In addition to acetylation, glycolysis also indirectly affects methylation through serine biosynthesis that utilizes 3-phospho-glycerate (3-P-G) as the starting material (19). Through one-carbon metabolism, serine is used for the de novo synthesis of methionine and SAM which is the co-substrate of methyltransferases (19, 20).
In this review, we summarize the recent research on the regulation of glucose metabolism and its associated metabolism by sirtuin proteins and their co-substrate NAD+ and their impact on epigenetic regulation of MΦ activation, polarization, and autophagy activity. We also discuss the NAD+-dependent sirtuin histone deacetylases as emerging drug targets for the treatment of infectious diseases, specifically for tuberculosis. Since we wish to focus on metabolism-derived co-substrates of histone acetylation/methylation enzymes and the NAD+-dependent histone deacetylase-sirtuin proteins, epigenetic regulation of autophagy by other histone modification enzymes or modification states is beyond the scope of this review and are covered elsewhere (21, 22).
Glucose metabolism and immune responses
Glucose metabolism exerts a strong impact on immune cell function (Figure 1) (23). For example, hexokinase (HK) binds to bacterium-produced N-acetylglucosamine and causes its deactivation as well as its dissociation from mitochondrial voltage-dependent anion channels (VDACs), which in turn, leads to NOD-Like Receptor family Pyrin domain containing 3 (NLRP3) inflammasome activation in MΦs (24, 25). Phosphoglucose isomerase (PGI) is identical to the protein known as Autocrine Motility Factor (AMF) which is upregulated in cancer cells together with other glycolysis enzymes and thought to play a key role in cancer metastasis by activating Epithelial-Mesenchymal Transition (EMT) and the MAPK/ERK or PI3K/AKT pathways (26, 27). These pathways are also upregulated in glucose deprived MΦs (28–30). Fructose-bisphosphate aldolase (FBA) is immuno-responsive during pathogen infection and is a potential vaccination target (31). Triosephosphate isomerase (TPI) catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P). TPI has been predicted to be essential for growth of MTB (32). Phosphoglycerate kinase (PGK) enhances the immunity to Streptococcus agalactiae in tilapia (33). Immunization of phosphoglyceromutase (PGM) induces Th1- and Th2-related immune responses in mice infected with Brucella (34). Deficiency of enolase (ENO1) causes the reduction of pyruvate which then contributes to a dysfunction in mitochondrial homeostasis and affects dendritic cell survival, maturation and antigen presentation (35). Pyruvate kinase 2 (PKM2) is required for the expression of PD-L1 in immune cells and tumors. Loss of PKM2 impairs endothelial cell proliferation and migration and triggers innate immune signaling (36). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) binds to 3’-UTR of inflammatory mRNAs and inhibits the translation of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) (37). PDK2/4 serves as a metabolic checkpoint for polarization of macrophages into the pro-inflammatory M1 phenotype (38). Though not generally characterized as a glycolytic enzyme involved in the 10 steps of glycolysis, lactate dehydrogenase (LDH) is elevated in pro-inflammatory immune cells to produce surplus lactate. Another glycolysis-related enzyme is glucose-6-phosphate dehydrogenase (G6PD) which acts at the first and the rate-limiting step of the pentose phosphate pathway (PPP). G6PD level is elevated in M1-MΦs and cells deficient in G6PD have a reduced ability to induce the innate immune response, thereby increasing host susceptibility to infection with pathogens (39). In addition to glycolytic enzymes, the glycolytic intermediates also play a significant role in the activation of the immune system. Pyruvate is reduced by LDH to form lactate that regulates immune response in macrophages and dendritic cells (40). Importantly, phosphoenolpyruvate (PEP), the precursor of pyruvate, is an immune signaling molecule; it promotes pro-inflammatory functions and activates T cells by regulating Ca2+-transportation and translocation of nuclear factor of activated T cells (NFAT) (41). Other metabolic enzymes and their products associated with glucose and immunometabolism have been reviewed elsewhere. These pathways include PPP (42), TCA cycle (43, 44), serine biosynthesis and one-carbon metabolism (45, 46), glutamine metabolism (47), and arginine metabolism (48).
Figure 1
Glucose metabolism, reactive oxygen (ROS) and reactive nitrogen species (RNS) production in macrophages, and their action on bactericidal function
Reduction-oxidation (redox) reactions occur in various metabolic processes including glycolysis, TCA cycle, but predominantly in the electron-transport chain (ETC) of mitochondria, which is essential for the generation of energy (ATP) for living cells. Oxidants, typically reactive oxygen species (ROS), are produced as the byproducts of redox reactions in ETC (49, 50). The major cellular redox reactions are conversions between NAD+ and NADH, NADP+ and NADPH, and FAD and FADH2. NAD+ is reduced/converted into NADH during glycolysis (two molecules) and in TCA cycle (three molecules) (51). NADH is re-oxidized to NAD+ by either lactate dehydrogenation (LDH) which catalyze the conversion of pyruvate into lactate, or by the ETC complex I through which, one proton and two electrons are released and ROS (O2.-) is formed when the electrons are added to O2 (51, 52). Paralleling the glycolysis initiating at glucose-6-phosphate, the pentose-phosphate-pathway (PPP) generates NADPH (from NADP+) from pentose as well as ribose 5-phosphate, a precursor for the synthesis of nucleotides. Similar to NAD+/NADH, NADPH is oxidized by NADPH oxidase (NOX) to be converted back to NADP+ and ROS is formed when the released electrons are added to O2 (53). NOX is a membrane-bound flavocytochrome, containing two molecules of heme and one molecule of flavin adenine dinucleotide (FAD) with a spectroscopic absorbancemax of 558 nm. For this reason, NOX is also referred to as flavocytochrome b558 which contains p22phox (α-subunit, the production of the CYBA gene) and NOX2/gp91phox (β-subunit, CYBB gene) (54). NOX is found in functional phagocytes including neutrophils, eosinophils, monocytes, dendritic cells, and macrophages (55). The third pair of redox reaction is FAD and FADH2, which are bound to succinate dehydrogenase complex (SDH). The substrate of SDH is succinate, an intermediate of TCA cycle, which is synthesized directly from succinyl-CoA. Succinate synthesis is enhanced in M1-MΦs due to the inhibition of TCA cycle (9). In addition, succinate can also be synthesized via glutamine-dependent anaplerosis or the γ-aminobutyric acid (GABA) shunt, which promotes and maintains polarization of M1-MΦs (56). SDH is a part of ETC Complex II, and mediates oxidation of succinate into fumarate. This reaction is coupled with the reduction of ubiquinone (UQ) to ubiquinol (UQH2) coupling with the oxidation of FADH2 to FAD. When high amounts of succinate are oxidized to fumarate under low oxidative phosphorylation conditions, electron flux moves in the opposite direction of ETC, from complex II toward complex I, leading to reverse electron transport (RET) and generating ROS (9, 57, 58) (Figure 2). The production of mitochondrial ROS is also mediated by immunoresponsive gene 1 (IRG1), which utilizes β-oxidation of fatty acids to generate ROS and improved activity of ETC increases ROS production in phagosomes thereby augmenting bactericidal activity (59). On the other hand, IRG1 is also called Aconitate Decarboxylase 1 (ACOD1), an important enzyme in the TCA cycle, which converts aconitate to itaconic acid that has a canonical antibacterial role through isocitrate lyase inhibition (60, 61). IRG1 is specifically up-regulated in LPS induced pro-inflammatory murine M1-MΦs (62, 63). ROS are essential for macrophages to fight against invasive pathogens through the M1-MΦ-dependent innate immune defense system, but they also play a critical role in signal transduction, differentiation, and gene expression (64, 65). In addition to ROS, cells generate oxidants through reactive nitrogen species (RNS). RNS are produced from the reaction of nitric oxide (•NO) with superoxide (O2•−) to form highly reactive peroxynitrite (ONOO−) (66) (Figure 2). NO is synthesized from arginine by NO synthase (NOS2/iNOS) (67, 68). Macrophages produce both ROS and RNS in response to phagocytosis and are required for killing of pathogens (69). The antimicrobial function of macrophages mainly depends on NOS2 and NOX2 genes which are upregulated in both murine and human M1-MΦs to generate abundant ROS and RNS (70–74). Therefore, M1-MΦs exhibit a high bactericidal function to defend against many intracellular pathogens including MTB (75). It has been noted that M1-MΦs have lower acidification rate and reduced proton pumping activity and thereby increased proton moving force compared to M2-MΦs; this facilitates M1-MΦs to generate ROS and efficiently control pathogens (76). Interestingly, NO also enhances the accumulation of itaconic acid in inflammatory cells increasing anti-bacterial activity (77); consequently, gene disruption of IRG1 reduces itaconic acid increasing the susceptibility to MTB infection and lung immunopathology (78). Paradoxically, for some pathogens, excess ROS can hijack host immune system and become favorable to pathogen survival (79). The mechanisms of ROS dependent hijack are not clear but inhibition of ETC complex I and regulation of TCA intermediates by NO may provide a plausible explanation (77, 80).
Figure 2
Metabolic profiles of mouse and human M1- versus M2-MΦs during tuberculosis infection
Metabolic gene expression profiling has revealed a biphasic metabolic behavior of MTB infection using an animal model (81). In the early phase post infection (up to 8 hr), the innate immune system is activated to generate proinflammatory cytokines including interleukin-1β (IL-1β), IL-6, IL-12, and TNF-α, predominantly in the M1-MΦs. In this early phase, glucose uptake aided by upregulated GLUT1 is accelerated and the genes of glycolysis are activated to increase the production of ATP and glycolytic intermediates and increase the consumption of NAD+. Concurrently, oxidative metabolism is down regulated indicated by a decrease in key enzymes of the TCA cycle and mitochondrial ETC complexes in mice exposed to MTB (82–84). However, as the infection progresses to 24 and 48 hr, post-infection, the M1- metabolic state of macrophages is reversed and an increase in TCA cycle and oxidative phosphorylation with dampened glycolysis are observed suggesting a switch towards M2-MΦs (9, 81, 85). These data are consistent with increased glycolysis and reduced TCA proteins in human M1-MΦs and switch towards M2-MΦs observed using proteomics analysis in our lab (86). Whereas most proteins of the ETC complexes II-IV were down-regulated, majority of proteins in complex I were up-regulated in human M1-MΦs (86).
Although many metabolic profiling studies have been done using mouse macrophages, recent studies are focusing on human macrophages (86–92). For example, mice are more susceptible to tuberculosis whereas nearly 90% of humans exposed to tuberculosis develop latent infection indicating a better control by their macrophages. In this direction, Gleeson, et al. identified that lactate derived from glycolysis-generated pyruvate, is increased in M1-MΦs when activity of TCA cycle is down-regulated, suggesting it as a key player during metabolic remodeling in MTB-infected human macrophages (93). Treatment of resting human macrophages with exogenous lactate caused a decrease in extracellular acidification rate while an increase of oxygen consumption rate (analogous to oxidative phosphorylation), resulted in an increased capacity to kill MTB possibly through autophagy (94). The same study also found that tuberculosis antimicrobial drugs, such as clofazimine, reshaped the immunometabolic profiles of MTB infected human macrophages towards oxidative phosphorylation similar to the effects of lactate (95).
On the other hand, Cumming, et al. found that in MTB-infected human monocyte-derived macrophages (nondifferentiated/resting state) both glycolysis and oxidative phosphorylation were suppressed leading to a state of metabolic quiescence resulting in a decrease of ATP production in mitochondria and a switch from dependency on glucose to fatty acids (88). This study suggested that MTB promoted polarization of macrophages towards M2-MΦs. We suggest that this discrepancy could arise when the starting monocyte-macrophage populations are different. Nonetheless, there seems to be a consensus that MTB infected human macrophages undergo a transition from M1-MΦs during early phase of infection to M2-MΦs during late phase similar to the mouse data (81).
Interestingly, pharmaceutical modulation with histone deacetylase inhibitor, suberanilohydroxamic acid (SAHA) promoted the glycolysis rate of human macrophages with increased production of pro-inflammatory cytokine IL-1β (a marker of M1-MΦs) and decreased production of anti-inflammatory cytokine IL-10 (a marker of M2-MΦs) during the early stage of MTB infection associated with enhanced T helper cell responses ex vivo (87). In this direction, we recently reported RNA-seq based transcriptomic data supporting metabolic profiling; genes of glycolysis, TCA cycle, and ETC complexes were all up-regulated that MTB infected in M1-MΦs at 24 hr post infection (6). We further demonstrated that human M1-MΦs expressed unique innate immune response genes to defend against tuberculosis through increased production of NO, accelerated autophagy- dependent killing of MTB and increased antigen presentation to T cells through an ATG-RAB7-cathepsin pathway (6). Taken together, these data indicate that MTB infection promotes naïve macrophage polarization progressively from M1-MΦ to M2-MΦ phenotype. The biphasic metabolic switch observed using ex-vivo MTB-infected human macrophages is similar to that of mouse macrophages infected with MTB. However, mice still develop progressive tuberculosis after aerosol infection with MTB unlike humans suggesting that differences in metabolic regulation of M1- vs. M2-MΦs may exist. For example, we found that sirtuins were differentially expressed by MTB infected M1 and M2-MΦs unlike similarly infected in mouse MΦs (96). The metabolic basis for the differences in the antimicrobial function of M1 vs. M2-MΦs is discussed below.
Glucose has a profound impact on immunometabolism and autophagy in human MΦs
Glucose metabolism and glycolysis are key players in inflammatory response (10). In mouse M1-MΦs exposed to pathogens, both glycolysis and GLUT1 expression are upregulated in the early phase of infection to facilitate rapid glucose uptake and consumption, resulting in eventual depletion of glucose, increased acidification of the microenvironment, both of which can be detrimental to proliferating pathogens (8, 9, 97). In addition to ROS/RNS discussed above, another bactericidal mechanism of macrophages is autophagy which is regulated by nutritional and metabolic states (98). Autophagy is generally induced by decreased availability of glucose or other nutrients such as amino acids (Figure 3A). In contrast, it can be stimulated by metabolites such as fatty acids and ammonia. Under nutrition-restricted conditions, glucose, acetyl-CoA, and amino acids are depleted, and NAD+ accumulates leading to an increase in the NAD+/NADH ratio (51) which in turn, regulates autophagy (99, 100). Several metabolic-sensor kinases also regulate this process (Figure 3A).
Figure 3
The target of rapamycin complex 1 (mTORC1) of the mTOR complex is a positive regulator of glycolysis and is activated in M1-MΦs. Whereas mTORC1 inhibits autophagy, inhibition of mTORC2 activates the process. Roberts et al. demonstrated that during glucose-limiting conditions, HK2 binds and inhibits mTORC1 thereby activating autophagy, whereas in glucose-repletion condition, glucose-6-phosphate (G6P) inhibits the binding of HK2 to mTORC1 to suppress autophagy (101, 102). Therefore, HK2 and G6P are pharmaceutical targets to induce autophagy in glucose-rich condition. Indeed, Metformin, a biguanide antidiabetic drug, lowers G6P in hepatocytes by activation of glucose phosphorylation, which is downstream of glycolysis and triggers autophagy (103, 104). This means that during early phase of infection of macrophages, high glucose intake produces excess ATP that activates ROS-dependent oxidative stress response and thereby up-regulated pro-inflammatory cytokines but this process also reduces autophagy without pharmacological intervention (102, 105). However, during the later phase of infection of macrophages, glucose and other nutrients are depleted, resulting in activation of autophagy and ROS level.
The second class of kinases is the AMP-activated kinases (AMPKs) which activate autophagy. Under glucose starvation, AMPK promotes autophagy by directly activating ULK1 through phosphorylation of Ser317 and Ser777 (106, 107), which can be prevented by mTORC1 that phosphorylates Ulk1 at Ser757 (106, 107). Glycolysis provides most of ATP in M1-MФs which is hydrolyzed into ADP and further into AMP, generating energy needed by cells. During glucose starvation, the AMP/ATP ratio increases leading to the activation of AMPK (108). Activation of AMPK inhibits mTOR resulting in an increase of autophagy (109, 110). Seemingly redundant to mTORC1, the RAS/cAMP-dependent protein kinase A (PKA) signaling pathway also regulates the induction of autophagy in yeast and mammals (111, 112). In addition to mTORC1 and PKA, Akt in the PI3K/Akt signaling pathway also regulates autophagy. Akt inhibits autophagy through phosphorylating the C-terminal Ser279 of Beclin-1 in the core autophagy machinery independent of mTORC1 (113, 114). Interestingly, during glutamine deprivation or hypoxia, a glycolytic enzyme – phosphoglycerate (PGK1), also directly phosphorylates the N-terminal Ser30 of Beclin-1 leading to enhanced VPS34 activity and subsequent autophagy (115). Of note, phosphorylation of Beclin-1 at N-terminus or C-terminus has different effects on autophagy; phosphorylation at the N-terminus enhances autophagy while at the C-terminus inhibits. Akt is a major mediator of insulin signaling and has been reported to be involved in mediating obesity and type 2 diabetes-related inflammatory disease (116). Metformin inhibits Akt activating autophagy, which is consistent with its activation of autophagy by lowering G6P as a result of inhibition of glucose flux and glycolysis (117). Deletion of Akt promotes macrophage polarization towards to M1-MФs and increased NO synthesis from arginine (118, 119). These observations suggest that, besides its antidiabetic effect, metformin can significantly reduce the risk of TB in patients with diabetes mellitus (120). Contradictory findings on the relationship between glucose metabolism and autophagy have been also revealed. Collins and coworkers reported that loss of mTORC1 in macrophages enhanced pro-inflammatory functions which are normally related to M1-MФs with upregulated glycolysis and activation of mTORC1 (121). These results were evaluated using rapamycin to polarize mouse and human macrophage models (122). The discrepancy can be explained by the differential localization of mTOR in lysosomes under M1- and M2- conditions (119). Mechanistically, it is known that, under starvation of glucose, a p38 MAPK-dependent pathway can trigger autophagy independent of the AMPK-mTOR pathway (123). We illustrate a diagram of Mtb-killing or survival during autophagy or nitric oxide (NO) through metabolite-sensing kinases corresponding to glucose homeostasis (Figure 3A).
During MTB infection of macrophages, glucose metabolism plays a significant role centered around autophagy. Glucose is a major metabolic source producing ac-CoA through glycolysis and SAM through serine biosynthesis and one-carbon metabolism. Ac-CoA and SAM are the necessary cofactors of histone acetyltransferases and methyltransferases (including DNA methyltransferases); further, glycolysis consumes NAD+ that is an essential cofactor of histone deacetylases. It is evident that glucose metabolism controls the level of cofactors and thereby, the epigenetic regulation through histone acetylation and methylation (and DNA methylation) which is further reviewed below. IFN-γ which drives M1-MΦs promotes a metabolic switch from oxidative phosphorylation to glycolysis, a process similar to the Warburg effect of hypoxia in cancer cells. Increased glycolysis causes the production and enrichment of copious lactate. Interestingly, Zhang et al. identified that histones can be modified by lactylation, and increased lactate promoted histone lactylation and polarization towards M2-MΦs (124). These data suggest that M1-MΦs can self-differentiate into M2-MΦs after prolonged glycolysis culminating in excess lactate. Noe et al. also show that glucose is still required for M2-MΦ polarization; under glucose starvation, exogenously added lactate matching the measured concentration of lactate produced by IL-4 primed M2-MΦs rescued the loss of lactate endogenously produced from glucose metabolism. This process enriched citrate from pyruvate by the half-blocked TCA cycle, and subsequently increased ac-CoA after ACLY cleavage resulting in global histone acetylation and M2 gene promoter-specific acetylation (Figure 3B) (125). Together, these observations indicate that lactate is a driver of M2 polarization from either M0- or M1-MΦs. Interestingly, the lactate-treated M2-MΦs had increased capacity to kill MTB possibly through autophagy (94). However, it remains unclear how histone lactylation is regulated and whether it causes histone acetylation to promote autophagy during TB.
Acetyl-CoA production from glycolysis is regulated by protein acetylation and sirtuins
Proteins acetylation dictates how cells choose glycolytic versus oxidative metabolism as a function of energy availability and then determine storage or utilization of carbon source (126, 127). Being a fundamental building block for fatty acid synthesis, ac-CoA is a necessary co-substrate of protein acetyltransferases to provide acetyl groups for acetylation of proteins, mostly on the ϵ-amino group of lysine, but also on the hydroxyl groups of serine, threonine, and tyrosine specifically among bacteria (128). Though it can be formed by fatty acid β-oxidation, amino acid catabolism, and break-up of citrate, ac-CoA is mainly produced by glycolysis (129, 130). Many enzymes in glycolysis, TCA cycle and proteins in mitochondria are the substrates of histone acetyltransferases whose acetylation sites have been identified by proteomics; nearly two-thirds of glycolic and TCA cycle enzymes show acetylation sites (14). Acetylation promotes or inhibits the activities of these enzymes, thereby increasing or decreasing the production of metabolites (129, 131). For instance, the enzymatic activity of phosphoglycerate mutase-1 (PGAM1), a protein critical for glycolysis, is regulated by glucose availability and SIRT1-dependent reversible deacetylation (15). When glucose is available, acetylation of PGAM1 stimulates catalysis. When glucose is restricted, SIRT1 levels increase, leading to deacetylation of PGAM1 and decrease in its enzymatic activity (15). Another positive correlation between acetylation and enzymatic activity is SIRT2 expression during iPSC reprogramming when OCT4 induces miR-200c-5p to suppress the expression of SIRT2 via microRNA binding sites in its coding sequence. As a result of downregulation of SIRT2, the activities of glycolytic enzymes (ALDOA, GAPDH, PGK1, ENO1 and PKM1/2) are increased due to elevated acetylation levels of these proteins (132). In contrast, acetylation of some glycolic enzymes can reduce their activity. It was reported that PKM2, a pyruvate kinase which is involved in the last step of glycolysis to produce pyruvate and ac-CoA, is acetylated at K305 by p300/(CREB binding protein) associated factor (PCAF) resulting in a decrease of its enzymatic activity (133). Moreover, acetylation of PKM2 enhanced its interaction with HSC70 and promoted its lysosome-dependent degradation via chaperone mediated autophagy under high glucose intake (133). Deacetylation at K305 by SIRT2 inhibits the pyruvate kinase of PKM2 by promoting its tetramerization (134), whereas deacetylation at K433 by SIRT6 inhibited the pyruvate kinase of PKM2 by suppressing its nuclear localization (135). The decrease of both enzymatic activity and protein level resulted in the accumulation of glycolytic metabolites upstream of PKM2, including FBP (fructose-1, 6-bisphophate) and G6P (glucose-6-phosphate). FBP was then found to couple with glycolytic flux to activate Ras and its downstream targets MEK and ERK driving autophagy (136); in contrast, G6P inhibited autophagy during glucose depletion (101, 102, 137). Interestingly, desuccinylation at K311 by SIRT5 counters acetylation at K355 and K433 to activate the pyruvate kinase of PKM2 by promoting its tetramer-to-dimer transition and nuclear localization, thereby blocking macrophage IL-1β production and preventing dextran sulfate sodium (DSS)-induced colitis in mice (138). These observations suggest that glucose metabolism and ac-CoA production are regulated by the acetylation states of glycolytic enzymes and sirtuin proteins play a major regulatory role.
Histone acetylation is responsive to metabolite levels and regulates autophagy
Acetylation of histones is a critical epigenetic modification that changes chromatin architecture and regulates gene expression. Many studies show that metabolism regulates acetylation, and, the changes in glucose metabolism can regulate histone acetylation (12, 13, 139). Using multiplexed stable isotopic labeling by amino acids in cell culture (SILAC)-based proteomics, Locasale’s lab found that the acetylation levels of half of identified histone acetylation sites and lysine acylation modifications at these sites were modulated by the rate of glycolysis and that histone acetylation levels were strongly correlated with ac-CoA levels and inversely associated with the ratio of ac-CoA to free CoA (11). However, glycolysis-generated, ac-CoA-dependent histone acetylation was competitively regulated by citrate-generated ac-CoA by ATP-citrate lyase (ACLY) (140–142). Moreover, the production of ac-CoA seems to be counter-balanced by utilization of ac-CoA to form lactate from pyruvate via LDH, reaction with OAA to form citrate entering the TCA cycle, acetylation of amino acids, and synthesis of fatty acids and other molecules in various metabolic pathways. Therefore, histone acetylation regulates metabolism and macrophage activation, whereas acetylation is fine-tuned by metabolism in polarized macrophages (143, 144). LPS/IFN-γ promotes polarization towards M1-MΦs characterized by up-regulated glycolysis and production of pro-inflammatory cytokines, such as IL-1β whose expression is enhanced by histone acetylation (145). The acetylation was thought to be due to the increased production of ac-CoA from elevated glucose metabolism and upregulated ACLY that reciprocally up-regulates glycolytic gene expression (146, 147). Higher levels of histone acetyltransferase MOF expression and acetylation at histone H4K16 were detected in inflammatory macrophages at the wound sites of diet-induced-obese mice compared to the anti-inflammatory macrophages in the healing phase (148). In addition, ACLY-mediated citrate metabolism in the TCA cycle contributes to the production of ROS and RNS in inflammatory cells (149). In contrast, Noe and co-workers reported that ACLY activation also promoted naive M0 to M2 polarization through the lactate-citrate-ac-CoA route for histone acetylation in tumor microenvironments (TME) (125). It remains unclear whether data from animal studies can be translated to humans although, some studies do reveal a positive correlation. For example, Vlad et al. found that histone acetylation, the expression of histone acetyltransferases p300, and the expression of NADPH oxidase-5 (Nox5) were all elevated in human atherosclerotic specimens. They were co-localized in the area of CD45+/CD68+ immune cells and lipid-rich deposits within atherosclerotic plaques (150); in these microenvironments, increased glucose intake and enhanced glycolysis were proposed (151). Consistently, ACLY was activated in inflammatory macrophages and human atherosclerotic plaques (152). In contrast, inhibition or silencing of Slc25a1, a transporter of citrate, resulted in decreased production of NO, ROS, and PGE2 in U937 cells (153) and inhibition of ACLY had the same effects (154). However, the role of ACLY in macrophage polarization was challenged by Namgaladze et al. who found that silencing ACLY expression using CRISPR/Cas9 in human THP-1 cells did not attenuate IL-4 induced gene expression as ACLY inhibitors did and concluded that ACLY might not be the major regulator of nucleocytoplasmic ac-CoA contributing to IL-4-induced M2-MΦ polarization of human macrophages (155). Erika Palmier and coworkers performed 13C tracing experiments using [U-13C]-glucose and glutamine and found that NO inhibited mitochondrial aconitase (ACO2) resulting in blockade of TCA, and that inflammatory macrophages rerouted pyruvate away from pyruvate dehydrogenase (PDH) in an NO-dependent but hypoxia-inducible factor 1α (HIF1α)-independent manner. This process promoted glutamine-based anaplerosis which sustained the TCA cycle using the glutamine generated αKG and OAA from pyruvate carboxylation (80). This suggested that ac-CoA generated from glycolysis would be reduced resulting in decreased histone acetylation in M1-MΦs due to NO-mediated inhibition of PDH. This is an intriguing cross regulation by NO in M1-MΦs that needs additional investigation. Besides production of ac-CoA from metabolism, histone acetyltransferases themselves also determine the acetylation level of histones and expression of autophagy genes. Fullgrabe et al. demonstrated that induction of autophagy by starvation or rapamycin inhibition of mTOX was coupled to reduction of histone H4 lysine 16 acetylation (H4K16ac) through downregulation of the histone acetyltransferase hMOF/KAT8/MYST1 in both mouse embryonic fibroblasts (MEF) and human transfected cells (156). However, downregulation of histone acetylation and hMOF also led to a transcriptional repression of autophagy genes based on a feedback mechanism, preventing chronic autophagy that could lead to cell apoptosis (156).
Sirtuins and NAD+ regulate protein/histone deacetylation and autophagy-mediated killing of bacteria
Sirtuins and antimicrobial mechanisms
Sirtuins, the class III histone deacetylases (HDAC), are crucial regulators of inflammation and immune cell metabolism and function (157–159). Metabolism is controlled not only by histone acetylation but also deacetylation. Activities of sirtuins are dependent of NAD+, NADH, or their ratio as NAD+ is their essential co-substrate (160). There are seven currently known sirtuins (SIRT1-7). Each sirtuin isoform is located at a specific compartment of the cell and has its specific preferred substrate. SIRT1, SIRT6, and SIRT7 are predominantly located in the cell nucleus (161). SIRT1 also exists in cytosol and is a master metabolic regulator and the most studied sirtuin protein so far; it is downregulated in cells with high insulin resistance and its overexpression increases insulin sensitivity (162–164). High concentration of glucose significantly downregulates SIRT1 expression at both mRNA and protein levels, which is related to upregulation of pro-inflammatory cytokines, IL-1β and TNF-α in RAW264.7 macrophages (165). On the other hand, SIRT1 is up-regulated under calorie-restrict conditions known to extend life-span (166, 167). SIRT1 also stimulates autophagy by deacetylating autophagy-related proteins (ATG) including ATG5, ATG7, and LC3 which are required for autophagy in cultured cells, embryonic and neonatal tissues (168, 169). SIRT1-dependent mechanism of autophagy induction is not clear; it may stabilize ATG proteins by forming a complex with them to prevent from degradation or prevent deacetylation at the promoters of ATG5 and ATG7 genes by other sirtuins due to its usage of NAD+ thereby activating expression of ATG5 and ATG7 (170). SIRT1 can also promote autophagy by activating AMPK to improve mitochondrial function (171), inhibiting the mTORC1 signaling pathway (172), and enhancing transcriptional activities of FOXO1 and FOXO3 through their deacetylation (169). Cheng and co-workers reported that MTB infection down-regulated SIRT1 in animal models and patients with active TB. Activation of SIRT1 by its activators, such as Resveratrol, not only induced autophagy but also dampened MTB-mediated chronic inflammation via deacetylation of RelA/p65 and impaired binding of RelA to the promoter of inflammatory genes (173). Similar results were obtained by others using mouse models (174). Another mechanism of the anti-TB property of SIRT1 was revealed by Yang, et al. who found that activation of SIRT1 prevented cell death in MTB-infected macrophages through BAX and GSK-3β (175, 176). In addition, SIRT1 activators also enhanced anti-TB drug efficacy (173). Interestingly, SIRT1 inhibition by sirtinol has also been reported to induce autophagy and autophagic cell death in MCF-7 cells (177). The mechanism is not known. Off target effects on NAD+ biosynthesis and/or salvage pathways is possible, since an enhanced activation of these pathways increases autophagy (178). SIRT6 is essentially a deacetylase of histones H3 and H4, which changes chromatin density and regulates gene expression and is required for normal base excision repair and double-strand break repair of DNA damage in mammalian cells (179). SIRT6, together with histone H3K9 methyltransferase G9a, participate in inflammatory response in macrophages, contribute to the IFN-sterol antiviral activity, and play an active role in inflammation-mediated glucose intolerance during obesity (180, 181). SIRT6 seems to facilitate MTB survival in macrophages by epigenetically modulating host cholesterol accumulation (182). SIRT7 was originally found to facilitate the transcription of DNA by DNA polymerase I, DNA polymerase II, and DNA polymerase III (183, 184). It has recently been found as a nutrient sensor similar to SIRT1 during glucose starvation or calorie-restricted diet and its depletion causes impaired activation of autophagy (185). The effects of SIRT7 on tuberculosis remain unclear.
SIRT2 is mainly cytoplasmic and also exists in nuclei where it can deacetylate histones. SIRT2 suppresses T cell metabolism by targeting key enzymes involved in glycolysis, TCA cycle, fatty acid oxidation, and glutaminolysis. SIRT2-deficient murine T cells and SIRT2 blockaded human tumor-infiltrating lymphocytes showed increased glycolysis and oxidative phosphorylation, enhanced proliferation and effector functions and thereby superior antitumor activity (186). SIRT2 dysregulated autophagy in high-fat-exposed mouse immune-tolerant and hypo-inflammatory macrophages (187). We found that the expression of SIRT2 was higher in MTB-infected human peripheral blood derived M2-MΦs which had lower autophagy activity than M1-MΦs infected with MTB (6). Pharmaceutical inhibition of SIRT2 increased autophagy and killing of MTB by M2-MΦs; morover, SIRT2 blockade combined with anti-TB drug dramatically increased MTB clearance in macrophages (6) (our unpublished data). Although Cardoso, et al. claimed that SIRT2 blockade only had a transient effect on MTB infection of mice (188), it is likely that human and mouse macrophages differ in sirtuin dependent regulation.
SIRT3, SIRT4, and SIRT5 are all found in the mitochondrial compartment and therefore implicated in regulating metabolic processes by deacetylating mitochondrial proteins. SIRT3 showed anti-inflammation property and mitigated endotoxin-induced acute lung injury (189). In MTB-infected macrophages, SIRT3 is down-regulated resulting in reduced expression of SIRT3-target genes including IDH2 and ETC complex I subunits and consequent accumulation of isocitrate, reduction of ETC complex I and II activity, lower GSH/GSSG ratio, and increase mtROS, promoting cell death (190). Paradoxically, activation of SIRT3 is necessary for autophagy and can provide protection for mitochondria in MTB-infected macrophages (191). However, anti-TB activity of SIRT3 is dependent on its genetic variants; for example, the minor allele genotype (A carriers) of rs3782118 shows a decreased risk of TB susceptibility, whereas the haptotype AGAAG (containing the major allete G of rs3782118) is associated with an increased risk of TB (192). SIRT4 is a mitochondrial ADP-ribosyltransferase that inhibits mitochondrial glutamate dehydrogenase 1 (GLUD1) activity, thereby downregulating insulin secretion in response to amino acids (193). SIRT4 shows opposite activity of SIRT1 and SIRT3 (194) and it counters SIRT1 and SIRT3 activity by suppressing their expression by rebalancing glycolysis and glucose oxidation during recovery of acute inflammatory response in monocytes (195).
SIRT5 exhibits multiple enzymatic activities, as it is a deacetylase, desuccinylase, and demalonylase, and capable of removing acetyl, succinyl, and malonyl groups from the lysine residues of proteins (196, 197). SIRT5 has dual functions of increasing ammonia production via promoting glutaminolysis and removing it by activating urea cycle. SIRT5 deacetylates and regulates carbamoyl phosphate synthetase (CPS1), the rate-limiting and initiating step of the urea cycle in liver mitochondria and therefore plays a critical role in ammonia detoxification (14, 197). On the other hand, SIRT5 stabilizes glutaminase (GLS) by desuccinylation, the enzyme transforming glutamine into glutamate generating ammonia (198). As ammonia is a diffusible regulator of autophagy (199), the regulation of autophagy by SIRT5 may be dependent on net ammonia concentration produced and consumed from glutaminolysis and urea cycle. Indeed, Polletta et al. demonstrated that in human breast cancer MDA-MB-231 and mouse myoblast C2C12 cell lines, ammonia production was increased when SIRT5 was silenced and decreased in SIRT5-overexpression cells (200). Morover, when GLS was activated by SIRT5, production of ammonia was increased and consequently autophagy activity was increased, whereas inhibition of SIRT5 decreased both ammonia production and autophagy (200). SIRT5 is therefore appears to be a potential regulator of autophagy and has additional, tangential effects like desuccinylation of mitochondrial proteins (201). Desuccinylation of ETC complex I and II occurs upon the binding of SIRT5 to the mitochondria-exclusive phospholipid-cardiolipin, which maintains the integrity of ETC residing on the inner mitochondrial membrane hence promoting the oxidation of NADH into NAD+ and the production of ROS and ATP (202, 203). Further, SIRT5 can desuccinylate glycolytic enzyme PKM2 causing its deactivation; in LPS activated but SIRT5 knock-out macrophages, IL-1β production was boosted due to an increase in succinylation of PKM2, demonstrating that SIRT5 is related to anti-inflammation (138). In our studies, we found that SIRT5 was up-regulated in MTB-infected and -uninfected human M1-MΦs in contrast to SIRT2 which was up-regulated in M2-MΦs (6). We found that both inflammatory IL-1β production and autophagy were up-regulated in MTB infected M1-MΦs unlike mouse macrophages (6, 96), and in contrast with Wang, et al. (138), we found that SIRT5 was related to a pro-inflammatory response. These issues underscore sirtuin- dependent differences between human and mouse macrophages. In cancer studies, SIRT5 was found to be downregulated in gastric cancer tissues and it enhanced autophagy via the AMP-activated protein kinase-mTOR signaling pathway (204). From these observations, we propose a tentative conclusion, though debatable, that of the seven sirtuin proteins, SIRT1, 3, 5, and 7 perform a protective function against infections with MTB whereas, SIRT2, SIRT4 and SIRT6 interfere with macrophage pathways facilitating pathogen survival. However, it is also likely that sirtuins are interdependent and compete with the shared resource of NAD+; for example, activity of one sirtuin protein may be enhanced by inhibition of another one. An example is that SIRT5 counters the inhibitory effects of SIRT2 and enhances the innate immune responses in macrophages by blocking SIRT2-dependent deacetylation of RelA/p65 activating NF-γB and increased production of downstream cytokines (205).
Sirtuins and arginine metabolism
An intriguing effect of SIRT5 is its ability to regulate arginine metabolism and NO production. As discussed above, SIRT5 deacetylates, desuccinates, and deglutarylates CPS1 to promote the formation of carbamoyl phosphate from ammonia in the urea cycle (196, 197). This process potentially increases the synthesis of citrulline because of interaction between carbmoyl phosphate and ornithine (Figure 4). Interestingly, acetylated glutamate (NAG) additively activates CPS1 (206). With the aid of catalytic enzyme arginosuccinate synthetase (ASS1), citrulline reacts with aspartate to form arginosuccinate which is then converted into arginine and fumarate by argininosuccinate lyase (ASL). Both ornithine and aspartate can be acetylated in macrophages. Therefore, it appears that acetylation of amino acids (glutamate, aspartate, and ornithine) and SIRT5 are involved in the conjugated urea cycle and arginine metabolism cycle. Nitric oxide, the RNS (reactive-nitrogen-species) precursor, is produced by arginine oxidation with the help of iNOS/NOS2. Increased citrulline can replenish arginine consumption for oxidation (207). We propose that an identification of the targets and functions of SIRT5 using mouse liver and human kidney cells can shed a light on the role of SIRT5 during macrophage activation and polarization.
Figure 4
In this direction, we measured mRNA expression of SIRT5 which was significantly higher in MTB-infected and uninfected M1-MФs than in M2-MФs cultured under identical conditions (6). Because we had detected that a majority of the proteins in the ETC complex I in M1-MФs was up-regulated (86), we suspected that not only desuccinylation by SIRT5 but also protein expression of ETC complex I promote NADH oxidation into NAD+ and ROS in M1-MФs. We also found an inverse relationship between acetylated amino acids and acetylated histones (86). Therefore, we speculated that acetylation of amino acids and acetylation of histones might compete for ac-CoA to fulfill acetylation; in M1-MФs, glycolysis generated acetyl-CoA cannot enter the partially blocked TCA cycle but is consumed by acetylation of amino acids as a consequence of which, the supply of ac-CoA for acetylation of histones is diminished. Another possibility is that histone acetylation was reduced by deacetylation with increased production of NAD+ by ETC complex I (86). Interestingly, acetylated aspartate (NAA), glutamate (NAG) and ornithine (NAO) were not only enriched in M1-MФs but were also connected with arginine metabolism (Figure 4). Both NAO and methylated arginine inhibit iNOS/NOS2 required for the production of NO (208–211). In addition, arginine is metabolized into citrulline releasing NO to form RNS that is upregulated in M1-MФs. These intriguing data led us to the tantalizing questions: how does SIRT5 regulate acetylation of amino acids and histones to leverage arginine metabolism and further, how is RNS production regulated by SIRT5 via arginine metabolism?
We note here that, bacteria including Mtb can synthesize arginine from glutamate by acetylation. NAG which is synthesized from glutamate by ArgA and NAO which is synthesized from NAG-5-semialdehyde by ArgD, are the important intermediates. Because mutation dependent loss of function for ArgA or ArgD led to antibiotic resistance in bacteria (212), it appears important to determine, how amino acid acetylation in macrophages is regulated by SIRT5 to replenish NAG and NAO during urea and arginine cycles in relation to drug resistance. Additional studies are warranted in this area.
Sirtuins and tryptophan metabolism
Deacetylation activities of sirtuins are regulated by the availability of NAD+. Two and three molecules of NAD+ are respectively consumed in glycolysis and TCA cycle. NAD+ can be recovered from NADH oxidation, pyruvate reduction to lactate, and the redox reaction in ETC complex I. NAD+ can also be de novo synthesized from tryptophan metabolism and synthesized via the nicotinamide salvage pathway (Figure 5). Thus, the overall level of NAD+ is well regulated under physiologic conditions to maintain optimal metabolism, appropriate energy production, and proliferation. Isotope tracing studies performed by Minhas et al. revealed that macrophage NAD+ was derived substantially from kynurenine pathway of tryptophan metabolism to maintain normal innate immune functions, whereas breakdown of this de novo NAD+ synthesis pathway could occur after LPS stimulation. They also demonstrated that inhibiting the expression of quinolinate phosphoribosytransferase (QPRT) decreased NAD+ level and caused innate immune dysfunction during aging and age-related diseases (213). Another study by Cameron et al. indicated that synthesis of NAD+ by the salvage pathway drove an immediate macrophage inflammatory response to LPS (214). The mechanistic insight was that LPS-induced ROS caused DNA damage through heightened expression of CD38 and increased PARP activity, a process which consumes NAD+ to trigger the salvage pathway for repletion of NAD+ (214, 215).
Figure 5
Using triomics to analyze IFN-γ activated but rested and uninfected human donor derived M1-MΦs, we found significantly increased expression of QRPT and the production of Niacin (aka, nicotinic acid or vitamin B3) which is the precursor of NAD+; this indicated up-regulated de novo NAD+ synthesis through tryptophan metabolism. We proposed that elevated NAD+ level would result in an increased deacetylation by sirtuins and thereby decreased histone acetylation. Indeed, we found decreased histone acetylation in uninfected M1-MΦs using mass spectrometric measurements (86). However, during MTB infection, we propose that NAD+ level could be depleted by glycolysis or the inhibition of NAD+ salvage pathway by tuberculosis necrotizing toxin (TNT) resulting in the death of macrophages (216, 217). Further, NAD+ replenishment alone or its combination with resveratrol (RSV) or cyclosporin A (CsA) can counter the toxicity of TNT and protect macrophages from MTB-induced cell death (173, 216, 218). Others found that NAD+ levels can also be raised by treatment with fatty acid oxidation inhibitors such as Trimetazidine (TMZ) which induced NADPH oxisase and autophagy mediated control of tuberculosis (219). Together, these data suggest that cellular NAD+ concentration controls both sirtuin deaceylase activity and antimycobacterial function of macrophages.
Pharmacological modulation of sirtuins to increase antimicrobial mechanisms
Sirtuins have been found as potential immunotherapeutic targets against tuberculosis because of their regulation of central energy metabolism via NAD+-dependent deacetylation. It has been reported that MTB infection depleted NAD+ level and perturbed sirtuin activity in MΦs (173, 190, 191, 217). Others reported that inhibition of SIRT2 with AGK2 restricted the growth of both dug-sensitive and -resistant strains of MTB and enhanced the efficacy of anti-TB drug Isoniazid in the mouse model of infection (220). In contrast, SIRT1 activators, such as resveratrol (RES), achieved a similar outcome by reducing lung pathology, chronic inflammation, and enhanced the efficacy of anti-TB drugs (173). As previously noted, hMOF is a specific histone H4K16 acetyltransferase; low activity of hMOF and low H4K16 acetylation is related to starvation-induced autophagy, which causes chronic repression of autophagic genes (156). SIRT1 is a H4K16 specific deacetylase. Mechanistically, activation of SIRT1 may keep the global H4K16 acetylation at low levels but on the other hand, it may deacetylate and activate ac-coA synthetase 1 (AceCS1) accumulating ac-CoA from acetate (221). Moreover, SIRT1 can also deacetylate hMOF to facilitate its binding to the chromatin at the promoters of autophagic genes promoting H4K16 acetylation due to increase in AceCS1 derived ac-Co-A (222). In murine J2-macrophages, the mRNA expression levels of SIRT1, SIRT3, SIRT5, and SIRT7 were all decreased at 24 hr post-infection of TB, which was also validated using mouse bone marrow derived macrophages (BMDM) (190). A detailed study of SIRT3 demonstrated that, over-expression of SIRT3 or treatment with SIRT3 activator Honokiol prevented MTB from inducing mitochondrial ROS accumulation in murine BMDM and cell death, whereas reduced expression of SIRT3 in Sirt3-/- mice increased bacterial burden (190). A similar report revealed that SIRT3 enhanced anti-TB defense through coordinated mitochondrial and autophagic functions (191). SIRT7 has protective effects against TB-infection through regulation of NO production and apoptosis demonstrated using an in-vitro model (223). Prakhar et al. observed restricted growth of TB and development of granulomatous lesion in the lungs and spleen of SIRT6 heterozygous mice infected with TB (182). Together these data suggest that the activators of SIRT3, SIRT5 and SIRT7 are potential anti-TB drugs in addition to the SIRT1 activator-Resveratrol. In contrast, we found that SIRT2 blockade increases autophagy-mediated killing of MTB. Of note, there are no data on whether SIRT4 contributes to anti-tuberculosis immunity.
Prospects for sirtuin modulators as drugs against tuberculosis
Despite reports that sirtuin inhibitors or activators in combination with the FDA-approved frontline anti-TB drugs enhance killing of drug resistant and dormant TB (173, 220, 224), none has been approved by FDA. Metformin is a direct SIRT1 activator based on computational modeling and experimental validation (225). Although it is not a TB-specific drug, it shows therapeutic efficacy for patients who have comorbidity of TB and diabetes and can be used as a pure adjunctive therapy for TB (226). Because, small chemical compounds that modulate sirtuin function have been pursued as anticancer agents (227), we propose that efforts should be made to use a combination of sirtuin activators and inhibitors to treat tuberculosis in combination with existing therapies.
Beside sirtuin proteins, the NAD+ biosynthesis pathway may also be a promising target for tuberculosis therapy. Recent elucidation of the mechanism of isoniazid (INH), a frontline anti-TB drug, indicated that INH couples with NADH catalyzed by KatG to form the active INH-NAD adduct, which in turn, binds tightly to the enoyl-acyl carrier protein reductase InhA so that the synthesis of mycolic acid for mycobacterial cell wall formation is inhibited (228). As MTB depends solely on its own de novo pathway to meet its NAD+ demand (229), MTB-QPRT provides an attractive target for designing novel anti-TB drugs (230). Coincidentally, NAD+ in the host M1-MΦ is significantly higher than M2-MΦs to maintain autophagy and bactericidal activity. Because of QPRT occurs in both host macrophages and MTB, its non-specific inhibition would decrease autophagy mediated killing capacity of macrophages. As crystal structures of both human and MTB derived QPRT have been elucidated (229, 231), to avoid toxicity, a drug to selectively target MTB-QPRT but not human-QPRT based on their structural difference at the substrate binding sites would be crucial. Quinolinic acid (QA) is the first intermediate in the de novo pathway of NAD+ biosynthesis that is common to all organisms and is mainly produced by the degradation of tryptophan in most eukaryotes. In contrast, in prokaryotes, including MTB, it is mainly produced from l-aspartate and dihydroxyacetone phosphate by the enzymes encoded by nadA (quinolinic acid synthetase) and nadB (l-aspartate oxidase) (232). Therefore, we propose that a drug to target nadA/B may be an alternative to QPRT inhibitors to control tuberculosis (233).
Sirtuins intersect the serine biosynthesis, one-carbon metabolism, and methylation of DNA and histones
The biosynthesis of serine starts with the oxidation of 3-phosphoglycerate (an intermediate from glycolysis) by NAD+ to 3-phosphohydroxypyruvate and NADH catalyzed by phosphoglycerate dehydrogenase (PHGDH), which is a rate-limiting enzyme (Figures 5, 6); the other two are Phosphoserine aminotransferase (PSAT) and Phosphoserine Phosphatase (PSPH). Since NAD+ is required for facilitating the functions of both PHGDH in serine biosynthesis and GAPDH in glycolysis, serine biosynthesis competes with the glycolysis pathway. Supporting this concept, serine deprivation in LPS-Simulated macrophages caused a reduction of pyruvate, decreased NAD+/NADH ratio, and decreased ROS level, partially resembling M2-MΦ phenotype but still maintaining a pro-inflammatory cytokine profile of M1-MΦs (234). However, Rodrigues et al. reported that serine is required for LPS induction of IL-1β mRNA expression but not inflammasome activation, because serine is used for conversion to glycine that is needed for macrophage GSH synthesis to support IL-1β production (235). Serine is required for the growth of MTB (236). Serine is converted to glycine by SHMT1 in the cytosol and SHMT2 in the mitochondria, which then donates one carbon to the folate cycle adjacent to the methionine cycle through methionine synthase (MTR) that in turn, requires vitamin B12 as a co-substrate. In the methionine cycle, SAM is synthesized from S-Adenosyl Homocysteine (SAH) with the donation of a methyl group from methionine. SAM is an essential co-substrate of methyltransferases, and provides the methyl group for methylation of histone, DNA and other biological compounds in the cells. In M1-MΦs, up-regulated glycolysis would increase the supply of 3-phosphohydroxypyruvate for serine biosynthesis. Because nitric oxide in M1-MΦs is toxic to vitamin B12, the transportation of B12 crossing the cell membrane is inhibited by hypoxia, and the mitochondrial citramalyl-CoA lyase (CLYBL) appears to be indirectly involved in the inhibition of vitamin B12 metabolism, depletion of B12 and as expected, subsequent inactivation of methionine synthase (MTR). As a result, one-carbon metabolism is hindered resulting in reduced formation of SAM and consequently, decreased methylation of histones or DNA (Figure 6). However, increased extracellular methionine uptake can still be triggered via the feedback mechanism to restore the loss. Excess methionine increases the production of SAM and DNA methylation attenuating LPS-induced inflammation (237). Because hypermethylation in macrophages reduces pro-inflammatory responses, we propose that a similar mechanism may favor the survival of MTB (238). Notably, MTB synthesizes its own methionine and SAM from homoserine which is produced through aspartate pathway (239, 240). Dinardo et al. performed methylation-sensitive enzyme-quantitative PCR (MSRE-PCR) and observed that in the PBMCs of TB-infected patients, pro-inflammatory genes including IL-1β and IFN-γ were DNA-hypermethylated resulting in dampened host immune responsiveness (4). MTB mediated hypermethylation of inflammatory genes is therefore a pathogen evasion strategy.
Figure 6
Proteomics has identified that the one-carbon enzyme, MTHFD1L (methylenetetrahydrofolate dehydrogenase [NADP+ dependent 1-like]) in the folate cycle, is a substrate for SIRT5 mediated desuccinylation/malonylation and SIRT5 also interacts with SHMT2 (241–244). In SIRT5 knock-down (KD) melanoma cells, reduced H3K4me3 and H3K9me3 were observed, indicating that reduced SAM production from impaired one-carbon metabolism of H3K4me3 and H3K9me3 sense the SAM levels in the cells (243, 245). SIRT5 also desuccinylates and activates SHMT2 to promote one-carbon metabolism and potential histone methylation in cancer cells (244). If this is true in immune cells, one-carbon metabolism and histone methylation would be enhanced in M1-MΦs as SIRT5 is up-regulated based on our RNA-seq data (6), which is opposite to what we proposed: that one-carbon metabolism would be down-regulated due to B12 depletion/MTR inactivation discussed above. In contrast, in breast cancer, SIRT2 regulates the reversible acetylation of PHGDH through TIP60 and promotes the binding of PHGDH and RNF5 to induce PHGDH degradation and reducing serine and glycine derived from glucose metabolism via the serine biosynthesis pathway in (246). If this information is also true in immune cells, in M2-MΦs upregulated SIRT2 would reduce serine synthesis from glucose metabolism potentially resulting in histone hypomethylation. Therefore, we propose that the methylation state in M1-MΦs versus M2-MΦ depends on which metabolic pathway is dominant- glycolysis and glucose intake, serine biosynthesis and intake, one-carbon metabolism and methionine intake, depending upon specific tissue microenvironments.
In order to understand how MTB regulates lysine and arginine methylation or other free amino acids and histones differently in M1- versus M2-MΦs, we will need to use isotope tracers and mass spectrometry. This will allow us to monitor how methyl migration to lysine and arginine residues from methionine/SAM produced by glucose derived serine occurs, and to determine whether serine is synthesized from intracellular source or directly taken up from extracellular nutrients in naïve versus polarized macrophages. Additionally, using isotope-labeled aspartate, we may be able to trace the methyl group migrating through the aspartate-homoserine-homocysteine route to lysine and arginine in MTB infected macrophages (Figure 6). We will then have a clear picture of methylation and epigenetic profiles differentially affected by metabolism in naïve or polarized macrophages infected with MTB.
Conclusion and perspectives
Glycolysis not only generates energy (ATP) to meet the demand of cells for their surviving but also controls the homeostasis of NAD+ which prevents cells from death and is an essential co-substrate of sirtuin proteins, the type-III histone deacetylases. Importantly, glycolysis is also a source of directly or indirectly producing ac-CoA and SAM, the co-substrates of histone acetyltransferases and methyltransferases respectively. Upregulated glycolysis in M1-MΦs generates increased ac-CoA from pyruvate and thereby increased histones acetylation which is counter-regulated by NAD+. NAD+ is consumed in glycolysis and TCA cycle and other redox processes. It is also reproduced by oxidation in metabolic pathways such as lactate synthesis from pyruvate and ETC. Increased NAD+ from de novo synthesis and the NAD+ salvage pathway would tip the balance towards hypoacetylation. Glycolysis also links to serine biosynthesis, a fuel for one-carbon metabolism, and the synthesis of SAM for histone/DNA methylation. Metabolic switch between M1- and M2-MΦs therefore causes an imbalance of co-substrates (ac-CoA and SAM) of histone acetyltransferases and methyltransferases thereby changing the landscapes of acetylation and methylation of histones and proteins in the metabolic pathways. Consequently, metabolism controls macrophage gene expression, the production of anti-mycobacterial oxidants, and autophagy during pathogen infection. Since the co-substrates produced by metabolites from glucose are regulated by other metabolic pathways, future work needs to be focused on the dynamic correlation between metabolism and histone modifications through measurement of the levels of co-substrates produced in polarized macrophages and the states of histone modifications on a time scale. For example, we can use stable isotope labeled glucose as the major probe during early and late phase of infection. It is also important to seek an insight into the impact of glucose metabolism on the expression of cytokines and autophagy genes regulated by co-substrates. Moreover, we can use stable-isotope labeled glutamine and arginine, to probe the mechanism of how sirtuin proteins control glutaminolysis and NO production through conjunction of the urea cycle and arginine metabolism cycle. Sirtuin proteins and their substrates are therefore promising targets for treatment of tuberculosis and likely other intracellular infections.
Statements
Author contributions
KZ wrote the manuscript. MS, EC, VS, BR, AK: contributed to supporting data and made graphics. CJ: Proposed the contents and edited the manuscript. All authors contributed to the article and approved the submitted version.
Acknowledgments
The authors wish to acknowledge funding support from NIH RO1 AI161015 (CJ, Janice Endsley, AK), AI138587 (CJ and Deepak Kaushal).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
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Summary
Keywords
human macrophages, autophagy, glycolysis, metabolism, histone modifications, SIRTUIN
Citation
Zhang K, Sowers ML, Cherryhomes EI, Singh VK, Mishra A, Restrepo BI, Khan A and Jagannath C (2023) Sirtuin-dependent metabolic and epigenetic regulation of macrophages during tuberculosis. Front. Immunol. 14:1121495. doi: 10.3389/fimmu.2023.1121495
Received
11 December 2022
Accepted
01 February 2023
Published
13 March 2023
Volume
14 - 2023
Edited by
Kai Wang, Southwest Medical University, China
Reviewed by
Tie Fu Liu, Fudan University, China; Dimin Wang, Zhejiang University, China
Updates
Copyright
© 2023 Zhang, Sowers, Cherryhomes, Singh, Mishra, Restrepo, Khan and Jagannath.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Kangling Zhang, kazhang@utmb.edu; Chinnaswamy Jagannath, cjagannah@houstonmethodist.org
This article was submitted to Inflammation, a section of the journal Frontiers in Immunology
Disclaimer
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