CORRECTION article

Front. Immunol., 05 May 2025

Sec. Viral Immunology

Volume 16 - 2025 | https://doi.org/10.3389/fimmu.2025.1594972

Corrigendum: Pharmacological potential of Withania somnifera (L.) Dunal and Tinospora cordifolia (Willd.) Miers on the experimental models of COVID-19, T cell differentiation, and neutrophil functions

This article is a correction to:

  1. Pharmacological potential of Withania somnifera (L.) Dunal and Tinospora cordifolia (Willd.) Miers on the experimental models of COVID-19, T cell differentiation, and neutrophil functions

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Zaigham Abbas Rizvi,*Zaigham Abbas Rizvi1,2*Prabhakar BabelePrabhakar Babele3Upasna Madan,Upasna Madan1,2Srikanth Sadhu,Srikanth Sadhu1,2Manas Ranjan Tripathy,Manas Ranjan Tripathy1,2Sandeep Goswami,Sandeep Goswami1,2Shailendra ManiShailendra Mani3Madhu Dikshit,*Madhu Dikshit3,4*Amit Awasthi,*Amit Awasthi1,2*
  • 1Immuno-biology Lab, Translational Health Science and Technology Institute, NCR-Biotech Science Cluster, Faridabad, Haryana, India
  • 2Immunology-Core Lab, Translational Health Science and Technology Institute, NCR-Biotech Science Cluster, Faridabad, Haryana, India
  • 3NCD, Translational Health Science and Technology Institute (THSTI), NCR Biotech Science Cluster, Faridabad, Haryana, India
  • 4Pharmacology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India

A Corrigendum on
Pharmacological potential of Withania somnifera (L.) Dunal and Tinospora cordifolia (Willd.) Miers on the experimental models of COVID-19, T cell differentiation, and neutrophil functions

by Rizvi ZA, Babele P, Madan U, Sadhu S, Tripathy MR, Goswami S, Mani S, Dikshit M and Awasthi A (2023) Front. Immunol. 14:1138215. doi: 10.3389/fimmu.2023.1138215

In the published article, there was an error in Figures 5A and 5D (FACS panel of Th0 & Th1; and Th0 & Th2) as published. The FACS images in Figures 5A and 5D (Th0 & Th2 panel) were originally generated by Upasna Madan, a graduate student, as part of a broader project investigating the in vitro immunomodulatory effects of eight herbal extracts. Initially, all experiments were conducted with multiple biological and technical replicates, intending to publish the findings in a single manuscript. However, during analysis, we determined that presenting the data in multiple manuscripts, each focusing on a specific herbal extract, would be more effective.

Figure 5
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Figure 5. Dose kinetics of WS response on in vitro differentiation of Th1, Th2 and Th17 cells from naïve CD4+ T cells. Sorted naïve CD4+ T cells from mouse spleen and lymph nodes were activated using soluble anti-CD3 antibody and differentiated into helper T (Th)2 (A, B), Th17 cells (D, E) and Th1 subtypes (G, H) by using different cytokines viz recombinant mouse IL-4; TGF-β + IL-6 and IL-12 cytokines respectively. WS was added in concentrations ranging from 10ug/ml to 1000ug/ml initially at the time of cell seeding. After 72 h of incubation IL-4, Il-17 and IFN-gamma production was measured respectively for Th2, Th17 and Th1 cells by intracellular cytokine staining. IC50 values were calculated using Graph pad prism software (C, F, I). ****P < 0.0001 by one-way ANOVA.

During this segmentation process, representative control images from the FACS analysis were inadvertently reused in two different manuscripts (PMID: 36960064 & PMID: 37765142). Since each experiment included multiple control replicates, we are in the process of replacing the affected control FACS plots. Importantly, as these FACS plots pertain only to control conditions, their replacement does not alter the overall findings, interpretations, or conclusions of either manuscript.

The corrected Figures 5A and 5D, Th0 & Th2 panel and its caption appear below.

The authors regret this oversight and affirm that it does not impact the scientific conclusions of the article. The original article has been updated accordingly.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: Withania somnifera (Ashwagandha), Tinospora cordifolia, SARS-CoV-2, hamster model, T cells, neutrophils, and hACE2 transgenic mice, COVID-19

Citation: Rizvi ZA, Babele P, Madan U, Sadhu S, Tripathy MR, Goswami S, Mani S, Dikshit M and Awasthi A (2025) Corrigendum: Pharmacological potential of Withania somnifera (L.) Dunal and Tinospora cordifolia (Willd.) Miers on the experimental models of COVID-19, T cell differentiation, and neutrophil functions. Front. Immunol. 16:1594972. doi: 10.3389/fimmu.2025.1594972

Received: 17 March 2025; Accepted: 11 April 2025;
Published: 05 May 2025.

Edited and Reviewed by:

Alfonso J. Rodriguez-Morales, Fundacion Universitaria Autónoma de las Américas, Colombia

Copyright © 2025 Rizvi, Babele, Madan, Sadhu, Tripathy, Goswami, Mani, Dikshit and Awasthi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Amit Awasthi, YWF3YXN0aGlAdGhzdGkucmVzLmlu; Madhu Dikshit, ZHJtYWRodWRpa3NoaXRAZ21haWwuY29t; bWFkaHUuZGlrc2hpdEBjZHJpLnJlcy5pbg==; Zaigham Abbas Rizvi, emFpZ2hhbS5hYmJhczE1QGdtYWlsLmNvbQ==

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.