Combined Complement and Coagulation Activation in ST-Elevation Myocardial Infarction: Associations with myocardial injury and dysfunction

Karsten Engseth Kluge^1,2*Sigrun Halvorsen^1,2Geir Øystein Andersen²Charlotte Holst Hansen²Ingebjørg Seljeflot²Theis Tønnessen^1,3Ida Gjervold Lunde^1,2,4Ragnhild Helseth²

• ¹University of Oslo, Oslo, Norway
• ²Oslo Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevål, Oslo, Norway
• ³Department of Cardiothoracic Surgery, Oslo University Hospital, Oslo, Norway
• ⁴KG Jebsen Center for Cardiac Biomarkers, Campus Ahus, University of Oslo, Oslo, Norway

Introduction: Preclinical data indicates reciprocal activation of the complement system and the coagulation cascade. The magnitude of this interaction in patients with acute myocardial infarction is unknown. We aimed to determine associations between circulating markers of complement and coagulation activation in patients with acute ST-elevation myocardial infarction (STEMI), and explore a possible link to myocardial injury and left ventricular dysfunction. Materials and methods: We included 864 patients with STEMI. Blood was drawn at a median of 18 hours after percutaneous coronary intervention. Complement activation was assessed by the terminal complement complex (TCC), and coagulation activation by prothrombin fragment 1+2 (F1+2), D-dimer and endogenous thrombin potential (ETP). Myocardial injury was estimated by peak troponin T (TnT), and left ventricular function was quantified on echocardiography by left ventricular ejection fraction (LVEF). Results: TCC was weakly correlated to F1+2 (r=0.086, p=0.012), D-dimer (r=0.176, p<0.001) and ETP (r=0.144, p<0.001). In multivariate binary logistic regression, there was no significant interaction between TCC and the coagulation markers on the risk of having high peak TnT or low LVEF. Conclusion: In this STEMI cohort, complement activation as measured by TCC was weakly associated with markers of coagulation activation, but the two systemsmeasured markers had no combined relation with the risk of high peak TnT or low LVEF. These findings suggest that while simultaneous activation of complement and coagulation cannot be ruled out, combined high levels of TCC and coagulation markers do not mirror simultaneous high activation of both systems is not relevant for the extent of myocardial injury or dysfunction in STEMI.