ORIGINAL RESEARCH article
Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1643366
This article is part of the Research TopicMolecular Pathways and Signaling Molecules in Cancer Therapy: Advances and InnovationsView all 10 articles
Identification and verification of the key genes involved in gallbladder cancer
Provisionally accepted
- 1The affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
- 2Wuxi Medical Center , Nanjing Medical University, Wuxi, China
- 3Wuxi People's Hospital, Wuxi, China
- 4General Surgery, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China
- 5General Surgery, Shenzhen Yantian District People's Hospital, Shenzhen, China
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Background: Gallbladder cancer (GBC) is a highly aggressive malignancy of the biliary tract. It often lacks distinct symptoms in its early stages, and no specific biomarkers have yet been identified for its diagnosis. Objective: To identify key genes involved in GBC pathogenesis using public databases and bioinformatics analysis and validate these findings experimentally, providing a foundation for developing potential GBC biomarkers Methods: Analysis of GBC-related data from the Gene Expression Omnibus database revealed that G protein-coupled receptor 64 (GPR64) was differentially expressed in GBC. GPR64 expression in GBC-SD and NOZ cells was modulated using lentiviral transfection. Functional assays assessed cancer-related phenotypes, while apoptosis was measured using flow cytometry. Xenograft models in nude mice were established with cell lines overexpressing GPR64. Results: GPR64 expression was reduced in GBC. Its overexpression suppressed GBC cell invasion, migration, and proliferation, and induced apoptosis. In vivo findings were consistent with in vitro results. Conclusion: GPR64 plays a critical role in GBC pathogenesis and may serve as a promising biomarker for its diagnosis and treatment.
Keywords: gallbladder cancer, GPR64, exosome, biomarker, bioinformatics
Received: 08 Jun 2025; Accepted: 20 Aug 2025.
Copyright: © 2025 Tang, Zhou, Lu, Cao, Zhang and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Dengyi Cao, General Surgery, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China
Yun Zhang, The affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Shaobo Zhou, General Surgery, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China
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