A Multi-Omics Analysis of Pancreatitis: Bridging Familial Genetics and Disease Progression

Fu Li¹Jin-xin Huang¹Wen-jie Sun²Jingqing Zeng³Ke-xin Gan¹Biao Gong¹Jian-mei Ji¹Jian Chen^4*Zhaohui Deng³Dongliang Xu¹

• ¹Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
• ²The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
• ³Shanghai Children's Medical Center Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
• ⁴Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China

Chronic and acute pancreatitis (CP and AP) are debilitating conditions with significant morbidity and mortality, necessitating a comprehensive understanding of their underlying mechanisms. Our study provides a high-resolution, multi-omics investigation into the genetic and immune-cell underpinnings of pancreatitis, integrating rare familial chronic pancreatitis with a large cohort of acute pancreatitis patients. Utilizing an integrative approach that combined whole-exome sequencing (WES) from two pediatric CP patients and their family members with single-cell RNA sequencing (scRNA-seq) and bulk transcriptomics from a public acute pancreatitis cohort (n=119), we identified a shared molecular and cellular pathology. WES of the CP family revealed heterozygous mutations in 12 novel genes, including EXOC4, ATG2A, and UNC80. Functional enrichment analysis highlighted autophagy, cell adhesion, and vesicle-mediated transport as key biological processes implicated in the pathophysiology of both conditions. Single-cell profiling of peripheral blood mononuclear cells (PBMCs) from the CP family revealed a marked increase in the proportion of naive B cells and an altered activity of CD8+ T cells, suggesting a dysregulated B-cell-mediated immune response. This observation was corroborated in the acute pancreatitis cohort, where CIBERSORT analysis revealed a significant increase in both naive B cells and CD8+ T cells correlating with disease severity. Weighted gene co-expression network analysis (WGCNA) on the AP cohort uncovered 14 gene modules associated with disease progression. These modules were significantly enriched for pathways central to the innate immune response, including complement-dependent cytotoxicity and neutrophil degranulation, providing a molecular link to the observed immune cell infiltration. An AI-driven model incorporating 110 CP family-related genes (GTCPFs) demonstrated exceptional predictive capability (average AUC > 0.84) for acute pancreatitis severity, highlighting the translational potential of our findings. The model identified a robust signature of 17 genes, including ATG2A, EXOC4, and TNS1, which may serve as novel diagnostic and prognostic biomarkers. Our findings provide a unified view of pancreatitis pathogenesis, linking novel genetic variants to specific immune-cell and transcriptomic signatures. This integrative approach underscores the critical importance of both genetic and immune factors in CP and AP, identifying potential biomarkers and therapeutic targets and paving the way for personalized medicine in managing these challenging conditions.