Correction: Anti-Lipid IgG Antibodies Are Produced via Germinal Centers in a Murine Model Resembling Human Lupus

Carlos Wong-Baeza^1*Albany Resendiz-Mora¹Luis Donis-Maturano²Isabel Wong-Baeza¹Luz Zarate-Neira¹Juan Carlos Yam-Puc²Juana Calderón-Amador²Yolanda Medina-Flores³Carlos Wong¹Isabel Baeza¹Leopoldo Flores-Romo^2*

• ¹National Polytechnic Institute (IPN), Mexico City, Mexico
• ²Cinvestav, Mexico City, Mexico
• ³Instituto de Diagnostico y Referencia Epidemiologicos, Mexico City, Mexico

Therefore, we deleted panel 3D and updated the letters on the remaining panels. The corrected Figure 3 appears below.The original version of this article has been updated. There was a mistake in the caption of Figure 3 as published. We used the same image to show the gating strategy (panel 3A) and the experimental result (panel 3D). The caption of Figure 3 with the mistake appears below:Region analysis shows the percentage of NPA-specific IgG + and IgG -plasma cells on days 10 (B) 20 (C) and 35 (D) in the bone marrow of mice that had been inoculated with NPA-liposomes. Percentage of NPAspecific IgG + and IgG -plasma cells on day 35 in the spleens of mice inoculated with saline solution (E) or with smooth liposomes (F). Percentage of IgG + or IgG -plasma cells specific to NPA in the draining lymph nodes (G, J), spleen (H, K) and bone marrow (I, L).Therefore, we deleted panel 3D, referred to panel 3A when describing the experimental result, and updated the letters on the remaining panels. The corrected caption of Figure 3 appears below.Region analysis shows the percentage of NPA-specific IgG + and IgG -plasma cells on days 10 (B) 20 (C) and 35 (A) in the bone marrow of mice that had been inoculated with NPA-liposomes. Percentage of NPAspecific IgG + and IgG -plasma cells on day 35 in the spleens of mice inoculated with saline solution (D) or with smooth liposomes (E). Percentage of IgG + or IgG -plasma cells specific to NPA in the draining lymph nodes (F, I), spleen (G, J) and bone marrow (H, K).The original version of this article has been updated. Adding/removing text We used the same image to show the gating strategy (panel 3A) and the experimental result (panel 3D). The paragraph with the mistake appears below: Specific plasma cells for NPA (Gr1 -, CD19 -, CD138 + , NPA + ) were identified by flow cytometry (Figures 3A -3D) in the draining lymph nodes, the spleen and the bone marrow of mice that received liposomes bearing NPA; these NPA-specific plasma cells were not observed in mice that received either smooth liposomes or saline solution (Figures 3E -3F). The percentage of IgG + NPA-specific plasma cells increased progressively, from 0.78%, 1.5% and 1.63% on day 10 to 2.67%, 7.04% and 7.27% on day 35 for the draining lymph nodes, the spleen and bone marrow, respectively (Figures 3G -3I). Concomitantly, the percentage of IgG -NPA-specific plasma cells decreased progressively from 0.75% and 7.2% on day 20 to 0.41% and 5.15% on day 35 for the draining lymph nodes and the spleen, respectively (Figures 3J -3K); and from 6.48% on day 10 to 2.19% on day 35 for bone marrow (Figure 3L).Therefore, we deleted panel 3D, referred to panel 3A when describing the experimental result, and updated the letters on the remaining panels. A correction has been made to the section [Results Section, subsection Plasma cells that produce IgGs specific to NPA are found in mice with the lupus-like disease, Paragraph 3]: Specific plasma cells for NPA (Gr1 -, CD19 -, CD138 + , NPA + ) were identified by flow cytometry (Figures 3A -3C) in the draining lymph nodes, the spleen and the bone marrow of mice that received liposomes bearing NPA; these NPA-specific plasma cells were not observed in mice that received either smooth liposomes or saline solution (Figures 3D -3E). The percentage of IgG + NPA-specific plasma cells increased progressively, from 0.78%, 1.5% and 1.63% on day 10 to 2.67%, 7.04% and 7.27% on day 35 for the draining lymph nodes, the spleen and bone marrow, respectively (Figures 3F -3H). Concomitantly, the percentage of IgG -NPA-specific plasma cells decreased progressively from 0.75% and 7.2% on day 20 to 0.41% and 5.15% on day 35 for the draining lymph nodes and the spleen, respectively (Figures 3I -3J); and from 6.48% on day 10 to 2.19% on day 35 for bone marrow (Figure 3K).The original version of this article has been updated. There was a mistake in Figure 4 as published. We used the same image in panels 4A and 4D to show the gating strategy (panel 4A) and the experimental result (panel 4D). The Figure with the mistake appears below: Therefore, we deleted panel 4D and updated the letters on the remaining panels. The corrected Figure 4 appears below.The original version of this article has been updated. There was a mistake in the caption of Figure 4 as published. We used the same image to show the gating strategy (panel 4A) and the experimental result (panel 4D). The caption of Figure 4 with the mistake appears below:Region analysis shows the percentage of NPA-specific germinal center B cells on days 10 (B), 20 (C) and 35 (D) in the spleens of mice that had been inoculated with NPA-liposomes. Percentage of NPA-specific germinal center B cells on day 35 in the spleens of mice inoculated with saline solution (E) or with smooth liposomes (F). Percentages of germinal center B cells specific to NPA in the draining lymph nodes (G) and spleen (H).Therefore, we deleted panel 4D, referred to panel 4A when describing the experimental result, and updated the letters on the remaining panels. The corrected caption of Figure 4 appears below.Region analysis shows the percentage of NPA-specific germinal center B cells on days 10 (B), 20 (C) and 35 (A) in the spleens of mice that had been inoculated with NPA-liposomes. Percentage of NPA-specific germinal center B cells on day 35 in the spleens of mice inoculated with saline solution (D) or with smooth liposomes (E). Percentages of germinal center B cells specific to NPA in the draining lymph nodes (F) and spleen (G).The original version of this article has been updated. Adding/removing text We used the same image to show the gating strategy (panel 4A) and the experimental result (panel 4D). The paragraph with the mistake appears below:Germinal center B cells specific for NPA (IgD -, CD19 + , PNA + , NPA + ) were identified by flow cytometry (Figures 4A -4D) in the draining lymph nodes and the spleen of mice that received liposomes bearing NPA; these germinal center B cells were not found in mice that received only smooth liposomes or saline solution (Figures 4E -4F). The percentage of NPA-specific germinal center B cells increased progressively, from 4.37% and 6.15% on day 10 to 16.37% and 19.87% on day 35 for the draining lymph nodes and the spleen, respectively (Figures 4G -4H).Therefore, we deleted panel 4D, referred to panel 4A when describing the experimental result, and updated the letters on the remaining panels. A correction has been made to the section [Results Section, subsection NPA-specific germinal center B cells are found in mice with the lupus-like disease, Paragraph 4]:Germinal center B cells specific for NPA (IgD -, CD19 + , PNA + , NPA + ) were identified by flow cytometry (Figures 4A -4C) in the draining lymph nodes and the spleen of mice that received liposomes bearing NPA; these germinal center B cells were not found in mice that received only smooth liposomes or saline solution (Figures 4D -4E). The percentage of NPA-specific germinal center B cells increased progressively, from 4.37% and 6.15% on day 10 to 16.37% and 19.87% on day 35 for the draining lymph nodes and the spleen, respectively (Figures 4F -4G).The original version of this article has been updated.