ORIGINAL RESEARCH article
Front. Immunol.
Sec. Alloimmunity and Transplantation
This article is part of the Research TopicThe Significance of Induced Pluripotent Stem Cells in Translational MedicineView all 5 articles
Deciphering Reprogramming Efficiency in human induced Pluripotent Stem Cells: Insights from the Generation of 150 Cell Lines
Provisionally accepted
- Institut d'Investigacio Biomedica de Bellvitge, Barcelona, Spain
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The discovery of induced pluripotent stem cells (iPSCs) revolutionized the field of translational medicine by enabling the reprogramming of adult somatic cells into a pluripotent state. From personalized disease models to innovative cell therapies, iPSCs are poised to play a central role in the future of clinical medicine. iPSCs hold enormous promises due to their ability to self-renew indefinitely and differentiate into all somatic cell types, thus offering patient-specific cellular models and therapeutic options without the ethical constraints of embryonic stem cells (ESCs). iPSCs, which exhibit pluripotency similar to embryonic stem cells, are generated by introducing specific factors into terminally differentiated cells, inducing a shift in their epigenetic and transcriptional landscape, which leads to the reactivation of the pluripotency program of the cells. Nevertheless, the mechanisms underlying successful reprogramming remain poorly understood. In this study we performed a statistical evaluation of reprogramming efficiencies of 150 iPSC lines generated in our lab, comparing factors such as the starting somatic cell type, passage number, donor´s health status, donor age and sex, reprogramming methodology, and growth conditions. We found that the most relevant factor This is a provisional file, not the final typeset article influencing reprogramming efficiency is the developmental status of the starting cells. While other parameters may exert minor effects, inherent donor-specific biological characteristics appear to play the strongest role in determining reprogramming outcomes. 213 words.
Keywords: Induced Pluripotent Stem Cells, integrating reprogramming methods, iPSC characterization, non-integrating reprogramming methods, Reprogramming efficiency
Received: 05 Oct 2025; Accepted: 17 Dec 2025.
Copyright: © 2025 Kuebler, Selvitella, Aran, Raya and VEIGA. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Bernd Kuebler
ANNA VEIGA
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