ORIGINAL RESEARCH article
Front. Immunol.
Sec. Mucosal Immunity
This article is part of the Research TopicHost-microbiota immuno-interactions for personalized microbial therapeutics: Volume IIView all 7 articles
Preparation and Mechanism Study of Alhagi Honey Polysaccharide-Aluminum Pickering Emulsion Adjuvant for Improving Intestinal Mucosal Immune Function
Provisionally accepted
En Zhou1Jiubin Du2Yongbin Zhai1Yan Xiao2Guangyan He1Dilinuer Abudureheman1Saifuding Abula1
Shengyi Wang1
Adelijiang Wusiman1*- 1College of Veterinary Medicine, Xinjiang Agricultural University, Ürümqi, China
- 2Tiankang Biopharmaceutical Co., Ltd, Urumqi, China
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Bovine viral diarrhea virus (BVDV) can induce diarrhea and mucosal tissue damage, and in severe cases, may result in fatal. Targeted adjuvants capable of enhancing intestinal IgA antibody responses represent an effective strategy for preventing bovine viral diarrhea. In this study, an Alhagi honey polysaccharide Pickering emulsion (AHPPE) adjuvant was developed by incorporating Alhagi honey polysaccharide (AHP) and retinoic acid (RA) with an aluminium-based adjuvant. Mice were subsequently immunized via intramuscular injection to evaluate the adjuvants' immunostimulatory potential. The results demonstrate that the particle size of AHPPE is 2133 nm, with excellent dispersion and stability. The encapsulation efficiencies for BVDV and AHP were 66.8% and 73.2%, respectively. AHPPE demonstrated recruitment of antigen-presenting cells at the injection site and activated IgA cells in the duodenum, jejunum, and ileum, inducing to increased IgA expression across multiple intestinal segments. Furthermore, AHPPE significantly induced serum IgG production and elevated levels of cytokines, including IL-4, IL-10, IL-17, IFN-γ, and TNF-α (P<0.05). Through sequencing analysis, it was found that IgA production may be induced via Intestinal immune network for IgA production, thereby mediating intestinal mucosal immune responses. Collectively, these findings indicate that AHPPE adjuvant administered via injection can simultaneously induce effective systemic immune and intestinal mucosal immunity. Therefore, as a novel intestinal-targeted adjuvant, AHPPE shows potential to enhance the specific intestinal mucosal immunity and systemic immunity of BVDV vaccine.
Keywords: Alhagi honey polysaccharide, mechanism of action, Pickering emulsions, Retinoic acid, Vaccineadjuvants
Received: 25 Dec 2025; Accepted: 03 Feb 2026.
Copyright: © 2026 Zhou, Du, Zhai, Xiao, He, Abudureheman, Abula, Wang and Wusiman. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Adelijiang Wusiman
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