Abstract
Salmonella enterica serotypes are invasive enteric pathogens spread through fecal contamination of food and water sources, and represent a constant public health threat around the world. The symptoms associated with salmonellosis and typhoid disease are largely due to the host response to invading Salmonella, and to the mechanisms these bacteria employ to survive in the presence of, and invade through the intestinal mucosal epithelia. Surmounting this barrier is required for survival within the host, as well as for further dissemination throughout the body, and subsequent systemic disease. In this review, we highlight some of the major hurdles Salmonella must overcome upon encountering the intestinal mucosal epithelial barrier, and examine how these bacteria surmount and exploit host defense mechanisms.
Salmonella Biology
Salmonellaentericaare Gram (−) bacteria responsible for causing typhoid disease and gastroenteritis. S. enterica serovar Typhi (S. Typhi) is the primary cause of typhoid fever, while non-typhoidal Salmonella (NTS) strains can cause Salmonella-induced food poisoning called salmonellosis. While young children, the elderly, and immuno-compromised individuals are most at risk for complications, people at any age are susceptible to the diarrhea, intestinal cramping, and intestinal epithelial erosion associated with salmonellosis. The disease is primarily spread by the contamination of water and food items with fecal matter from infected hosts and is often self-limiting, but can cause prolonged complications (Graham et al., 2000).
Salmonella possess Salmonella pathogenicity islands (SPI), or collections of pathogenesis-related genes acquired horizontally. At least 21 SPIs have been identified in S. Typhimurium and S. Typhi combined (for review of functions, see Sabbagh et al., 2010). In S. Typhimurium and in S. Typhi, SPI-1 and SPI-2 contain genes for two type-three secretion systems (T3SS). Specific to Gram (−) bacteria, the T3SS likely evolved from the flagella basal body, and is composed of a motor, needle complex, and translocon through which secreted effectors are injected into host cells (Stebbins and Galan, 2003). The effectors provide various functions, including promoting bacterial entry, controlling inflammatory responses, and regulating bacterial survival within the cell (summarized in Table 1). The SPI-1 T3SS (T3SS1) is primarily associated with invasion (Galan, 1996). Effectors secreted through the SPI-2 T3SS (T3SS2) seem to primarily promote the intracellular survival of Salmonella, although they may not be absolutely required for S. Typhi survival within human macrophages (Forest et al., 2010). However, in S. Typhi infections of humanized non-obese–diabetic mice, the loss of some SPI-2 genes caused a competitive disadvantage (Libby et al., 2010). Also, SPI-2 genes are up-regulated during S. Typhi infection of macrophages in vitro (Faucher et al., 2006). While the functions of SPI-1 and SPI-2 effectors have traditionally been considered distinct from each other, there is increasing evidence suggesting overlap in the times at which effectors from each system are required (Lawley et al., 2006; Brawn et al., 2007). These data highlight the intricate level of coordination between SPI-1 and SPI-2 involved with Salmonella pathogenesis. Nonetheless, recent data challenges the dependence of T3SS1 in Salmonella invasion (Radtke et al., 2010). SPI-1 mutant strains of Salmonella were capable of invading HT-29 3D intestinal cells to a higher degree than HT-29 monolayers, although the level of invasion was less than that of the wild-type strains. Additionally, SPI-1 was required for wild-type levels of intracellular replication over a 24-h period. The additional loss of SPI-2 may enhance these phenotypes. These data suggest that various pathways are required for wild-type levels of invasion and intracellular replication. Other types of secretion systems that may promote pathogenicity have been identified in additional SPI loci (Sabbagh et al., 2010).
Table 1
| Effector | Location | Function | Targets | T3SS apparatus |
|---|---|---|---|---|
| AvrA | SPI-1 | Controls Salmonella-induced inflammation (Collier-Hyams et al., 2002; Ye et al., 2007). | IkBa, beta-catenin | 1 |
| SipA | SPI-1 | Mediates invasion at apical surface by inducing actin bundling and promotes neutrophil migration to the apical surface. Also suspected to promote SCV formation. Undergoes cleavage by CASPASE-3 (Zhou et al., 1999; Brawn et al., 2007; Wall et al., 2007; Srikanth et al., 2010) | Actin | 1 |
| SipB | SPI-1 | Component of T3SS1 translocon (Kaniga et al., 1995; Hayward et al., 2000) | Cholesterol | 1 |
| SipC | SPI-1 | SPI-1 translocon component, induces actin bundling to promote invasion (Kaniga et al., 1995; McGhie et al., 2001) | Actin | 1 |
| SipD | SPI-1 | Component of T3SS1 translocon (Lara-Tejero and Galan, 2009) | 1 | |
| SopA | E3 ubiquitin ligase that may promote escape from SCV and promotes neutrophil migration. Also required during invasion (Wood et al., 2000; Raffatellu et al., 2005; Zhang et al., 2006) | 1 | ||
| SptP | SPI-1 | Similar to GAPs, and is a tyrosine phosphatase. Reverses pro-inflammatory responses due to other Salmonella effectors (Stebbins and Galan, 2000) | Cdc42, Rac-1 | 1 |
| SopB | SPI-5 | Inositol polyphosphate phosphatase that promotes macropinocytosis, regulates SCV localization, and promotes fluid secretion (Norris et al., 1998; Hernandez et al., 2004) | Inositol phosphates | 1 |
| SpiC | SPI-2 | Helps regulate T3SS2 secretion (Yu et al., 2002) | Hook 3, TassC | 2 |
| SseF | SPI-2 | SCV regulation (Abrahams et al., 2006) | Microtubules | 2 |
| SseG | SPI-2 | SCV positioning (Abrahams et al., 2006) | Microtubules | 2 |
| SopD | Promotes invasion and fluid secretion (Zhang et al., 2002; Raffatellu et al., 2005) | 1/2 | ||
| SopE | Bacteriophage | Promotes membrane ruffling and disrupts tight junctions (Hardt et al., 1998; Boyle et al., 2006) | Rac-1, Cdc42 | 2 |
| SopE2 | Promotes membrane ruffling and disrupts tight junctions (Stender et al., 2000; Boyle et al., 2006) | Cdc42 | 2 | |
| SspH1 | Bacteriophage Gifsy-3 | E3 ubiquitin ligase (Rytkonen and Holden, 2007) | 1/2 | |
| SspH2 | SPI-12 | E3 ubiquitin ligase (Quezada et al., 2009) | 2 | |
| PipB2 | Promotes Sif extension (Knodler and Steele-Mortimer, 2005) | Kinesin-1 | 2 | |
| SifA | Sif formation and membrane integrity (Stein et al., 1996; Beuzon et al., 2000) | SKIP, Rab7 | 2 | |
| SopD2 | Sif formation and promotes bacterial replication in mouse macrophages (Jiang et al., 2004) | 2 | ||
| SseJ | Negatively regulates Sifs and antagonizes SifA-mediated stability of SCV (Ruiz-Albert et al., 2002) | Cholesterol | 2 | |
| SseL | Cysteine protease and has de-ubiquitinating activity. Helps attenuate Salmonella virulence (Rytkonen et al., 2007) | 2 | ||
| SteC | A kinase that promotes F-actin meshwork formation (Poh et al., 2008) | 2 | ||
| SpvB | pSLT (in S. Typhimurium) | Depolymerizes actin filaments in vitro (Lesnick et al., 2001) | Actin | 2 |
| SpvC | pSLT (in S. Typhimurium) | A phosphothreonine lyase required for complete virulence in murine models (Mazurkiewicz et al., 2008) | 1/2 |
Salmonella effectors and their roles in pathogenesis.
A summary of effectors whose functions in Salmonella pathogenesis have been identified.
Salmonella Interaction with the Intestinal Mucosal Epithelia
The gastrointestinal tract is the largest mucosal surface in the human body, with the epithelial monolayer surface area alone measuring 400 m2 (MacDonald and Monteleone, 2005; Turner, 2009). Regulating the host microbiota, immune responses, and barrier functions is paramount to providing timely and controlled retaliation to pathogenic assaults, while simultaneously maintaining a healthy, balanced, intestinal environment (Figure 1).
Figure 1
Gut microbiome
The human intestine is home to a plethora of mutualistic microorganisms, dominated mostly by Lactobacillus, Bacteroides, and Firmicutes (Backhed et al., 2005). Among other roles, these organisms assist immune system development and promote epithelial homeostasis (Rakoff-Nahoum et al., 2004; Mazmanian et al., 2005). Beyond these regulatory roles, the microbiome also functions as a critical barrier to invading pathogens. The population of host microbes in the gut physically blocks pathogen access to the epithelial layer, and also outcompetes pathogens for nutrients, thus reducing the survival and invasiveness of intestinal pathogens. Nevertheless, S. Typhimurium is capable of maneuvering through the microbiome to reach intestinal epithelial cells. In mouse experiments with wild-type and avirulent S. Typhimurium, Stecher et al. demonstrate that an inflammatory state in the intestines not only permits wild-type S. Typhimurium to outcompete the microbiome during colonization, but also allows a normally avirulent strain to colonize the intestines in the presence of intestinal microbiota (Stecher et al., 2007). These data unveil an intriguing concept that S. Typhimurium utilizes inflammation to outcompete host microbiota in mouse models of salmonellosis.
Host microbiota may also regulate the degree of S. Typhimurium shedding by infected mice. Following infection with 108 CFU of bacteria, mice were classified into three groups corresponding to the level of fecal shedding: supershedders (>108 CFU/g feces), moderate shedders (104–108 CFU/g feces), and low shedders (<104 CFU/g feces). Treating low shedders with streptomycin converted these mice to supershedders (Lawley et al., 2008), suggesting that the absence of host microbes may have permitted Salmonella to colonize more heavily, thus elevating the level of shed bacteria. The significance of a gut microbiota barrier to Salmonella infection was also explored by Croswell et al. (2009). The authors found that treatment of mice with streptomycin, streptomycin–bacitracin, or ampicillin–vancomycin–neomycin–metronidazole (AVNM) reduced gut microflora populations, and that each treatment was subsequently associated with an increase in Salmonella colonization and Salmonella-induced inflammation compared to untreated mice. The authors further show that allowing 3 weeks between antibiotic treatment and infection still predisposed mice to greater levels of inflammation in response to Salmonella infection than was seen in mice that were never treated with antibiotics. These results suggest that antibiotics have long-lasting effects on gut microflora populations, and that the presence and composition of gut microfloral populations may play an important role in controlling Salmonella colonization.
Additionally, Barman et al. (2008) showed that salmonellosis may alter the microfloral population in FvB mice. Infected mice showed disrupted numbers of Bacteroides, Lactobacillus/Enterococcus, and Eubacterium rectale/Clostridium coccoides groups and of the C. perfringens group compared to uninfected mice. However, Salmonella did not replace these populations, and wild-type population numbers returned after Salmonella clearance, suggesting any effect salmonellosis has on the intestinal microbiota is not permanent.
Mucosal layer
Key to the mucosal epithelium is the formation of a mucus layer along the luminal lining of the gastrointestinal tract. Mucosal epithelial cells, specifically goblet cells, secrete glycosylated transmembrane proteins called mucins at the cell surface. These proteins generate a layer of large complexes containing thread-like structures and oligosaccharides. The resulting gelatinous layer blocks contact between the underlying epithelial monolayer and large particles, including bacteria. The mucus layer also contains trefoil factors, which are peptides produced in tissues containing mucus-producing cells, such as the intestinal epithelia. Their various proposed functions include limiting intestinal inflammation (Playford et al., 1996) and regulation of immune system responses (Baus-Loncar et al., 2005). Adhering to the mucosal layer would be necessary for Salmonella to avoid being washed out of the intestines. Indeed, there is evidence of Salmonella binding to mucus, although increased adherence does not necessarily correlate with increased mucosal penetration (Nevola et al., 1987; McCormick et al., 1988; Vimal et al., 2000).
Epithelial monolayer: M cells and the associated peyer's patches
The epithelial monolayer mediates interactions between triggers of immune responses, and the gut-associated lymphoid tissue (GALT), where immune responses originate (MacDonald and Monteleone, 2005; Turner, 2009). A follicle-associated epithelial (FAE) layer of columnar epithelial cells blankets lymphoid tissue within the intestinal wall. The intestinal mucosa contains a variety of cell types with unique functions including enterocytes, entero-endocrine cells, goblet cells, Paneth cells, and microfold (M) cells (Table 2). While all the unique epithelial cells found within the intestine are important for establishing the functional intestinal mucosal epithelium, in mice the M cells are a key route of invasion by Salmonella, and are thus discussed in greater detail here.
Microfold cells are specialized intestinal epithelial cells that are found in the FAE overlying mucosa-associated lymphoid tissue (MALT). Their primary role is to sample mucosal contents and transfer antigens from the lumen to underlying Peyer's patches (Tam et al., 2008), and thus act as sentinels of the intestinal epithelium. The overall structure of M cells differs in various ways from that of enterocytes. For example, the apical (mucosal) surface of M cells is not covered by the mucus layer observed over other cells in the intestines (Frey et al., 1996). Additionally, the apical brush border characteristic of enterocytes is absent from M cells, which instead contain microfolds. These features promote the sampling role of M cells, but also inadvertently provide opportunities for enteropathogen docking and invasion.
Table 2
| Cell type | General function | Salmonella infection impact |
|---|---|---|
| Goblet cell | Sustained and environmentally triggered mucin production (Deplancke and Gaskins, 2001) | Loss of goblet cells reported as a result of S. Typhimurium-mediated colitis in mice (Hapfelmeier et al., 2004, 2005), although this observation is not restricted only to colitis caused by Salmonella. Invasion of goblet cells reported in Salmonella infections of pig ileal loops (Meyerholz et al., 2002; Meyerholz and Stabel, 2003). |
| Paneth cell | Secrete the anti-microbial peptides lysozyme and alpha-defensin (Ayabe et al., 2000) | S. Typhimurium may modulate the production of anti-microbial peptides (Salzman et al., 2003) |
| M cells | Transfer antigens to Peyer's patches (Tam et al., 2008) | M cells are the preferred route of invasion by Salmonella (Jensen et al., 1998) |
| Enterocytes | Absorptive/villus enterocytes: Nutrient absorptionCrypt enterocytes: Chloride and IgA secretion | Salmonella invasion of absorptive enterocytes reported in calf ileal loops (Frost et al., 1997), and in pig ileal loops (Meyerholz et al., 2002; Meyerholz and Stabel, 2003) |
| Entero-endocrine | Secretion of various hormone molecules, promote food digestion | Salmonella-specific responses undetermined |
Cells within the intestinal epithelial monolayer.
An additional trait of M cells that promotes both their role as intestinal sentinels and promotes enteropathogen invasion is their use of a transcytotic pathway to shuttle luminal contents to lymphoid tissue (Kraehenbuhl and Neutra, 2000). Following endocytosis of extra-cellular material (including invading pathogens), M cells transfer the endosomal contents to the basolateral (serosal) surface and to the underlying MALT. This action results in appropriate immune responses to infection. Likewise, bacteria engulfed at the apical surface can escape the M cell through this same pathway and disseminate to other areas.
M cells provide a key route of invasion by S. Typhimurium (Jensen et al., 1998). SPI-1 genes help regulate Salmonella invasion through M cells, although SPI-1-independent processes are also proposed to regulate M cell invasion (Clark et al., 1996). For example, Salmonella defective for invA are less able to invade M cells (Clark et al., 1998), but SPI-1 mutants are still capable of invading M cells (Martinez-Argudo and Jepson, 2008). These results suggest that while SPI-1 genes are needed for wild-type levels of invasion, SPI-1-independent mechanisms are also important for Salmonella invasion through M cells.
The lymphoid tissue underlying M cells contains Peyer's patches, which are large aggregates of B lymphocyte follicles that contain germinal centers, and can be found along the length of the intestine. Here, the antigens translocated by M cells are processed by local dendritic cells. Lysozyme-producing dendritic cells associated with Peyer's patches mediate uptake of S. Typhimurium (LeLouard et al., 2010). Peyer's patches also play a role in up-regulating intestinal IgA as a result of Salmonella infection (Hashizume et al., 2008).
Epithelial monolayer: structure and salmonella entry
The barrier function of the epithelial monolayer results from a variety of proteins that maintain close intercellular interactions. Desmosomes are composed of cadherins, which form an adhesive interface (Koch and Franke, 1994; Green and Simpson, 2007), and varying structural proteins, notably plakoglobin and desmoplakin (Hatsell and Cowin, 2001), all of which help disperse forces from physical stress. Meanwhile, the paracellular pathway, or area between adjacent epithelial cells, is kept closed by tight junctions and adherens junctions. Bacteria attempting to penetrate the epithelial monolayer must possess mechanisms for overcoming these strong interactions. As such, Salmonella possess several effectors that mediate disruption of the epithelial barrier and subsequent uptake into the non-phagocytic epithelial cells lining the intestinal lumen. These actions are carried out largely through the alteration of junctional protein localization and of Rho GTPase activity. The latter mechanism is especially important in the Salmonella-mediated modulation of tight junctions. Dis-regulating the Rho-GTPases leads to changes in organization of junctions and of actin at the cell membrane, which subsequently alters the membrane morphology in a manner referred to as “ruffling” (Finlay et al., 1991), and promotes Salmonella entry (Figure 2).
Figure 2
Interactions between classical (Ca2+ dependent) cadherins and catenin family proteins are the foundation of adherens junctions. E-cadherin (epithelial cadherin) is a single pass transmembrane protein and its ability to homodimerize with E-cadherins on neighboring cells makes it a key component of adherens junctions (Takeichi, 1991). E-cadherins form small, intercellular clusters that quickly associate with actin and, overtime, expand into larger bundles that strengthen cell–cell adhesion (Adams et al., 1998). p120-catenin stabilizes E-cadherin at the cell surface, and its loss induces down-regulation of E-cadherin (Davis et al., 2003). Further, beta-catenin, a transcription factor involved in cell proliferation and differentiation, can bind to the cytoplasmic domain of E-cadherin (Hartsock and Nelson, 2008), and direct the localization of E-cadherin from the ER to the plasma membrane (Chen et al., 1999). Phosphorylation of beta-catenin promotes its ubiquitination and subsequent degradation (Aberle et al., 1997). Duan et al. (2007) demonstrated that cultured epithelial cells infected with S. Typhimurium display increased phosphorylated beta-catenin. This increase in phosphorylated beta-catenin resulting from Salmonella infection could increase the level of beta-catenin degradation, and thus could limit E-cadherin translocation to the plasma membrane. Such an action would result in weakened adherens junctions and promote Salmonella invasion through the epithelial monolayer.
The tight junction is an important regulator of epithelial monolayer permeability (Martinez-Palomo and Erlij, 1975), and of cell polarity by preventing mixing of apical (mucosal) and basolateral (serosal) components. The “leak pathway” permits passage of larger solutes, such as bacterial peptides (but not whole bacteria), while the “small pore pathway” excludes solutes larger than 4 Å, and exhibits some charge selectivity (Forster, 2008; Turner, 2009). The core of tight junction complexes is composed of occludin, ZO, and claudin proteins. Occludin binds to several crucial tight junction proteins including ZO-1 (Furuse et al., 1994), ZO-2 (Itoh et al., 1999), and ZO-3 (Haskins et al., 1998) and its activity is regulated by PKC-mediated phosphorylation (Andreeva et al., 2001). ZO-1, ZO-2, and ZO-3 are members of the membrane-associated guanylate kinase (MAGUK) family (Gonzalez-Mariscal et al., 2000). ZO-1 and ZO-2 assist in the polymerization of claudins, which permits extension of tight junctions, and also recruit ZO-3 to the tight junction (Umeda et al., 2006; Tsukita et al., 2009). A specific role for ZO-3 has yet to be determined, although it is shown to not be required for tight junction formation (Adachi et al., 2006). Claudins, which are tetraspan transmembrane proteins, are key structural elements, as they recruit occludin (Furuse et al., 1998). Another class of tight junction proteins include junction-associated adhesion molecules (JAMs), or which are integral membrane proteins that belong to the immunoglobin superfamily.
Cytoskeletal regulators of tight junctions include myosin ATPase, AMP-activated protein kinases, and especially Rho-GTPases (Turner, 2009). Rho-GTPases regulate various cell functions and are activated by guanine-nucleotide exchange factors (GEFs), which facilitate the active, GTP-bound state. GTPase activating protein (GAPs) inhibit Rho-GTPases by activating GTP hydrolysis, thereby inducing the inactive, GDP-bound state (Etienne-Manneville and Hall, 2002). Rho-GTPases are regulated by several proteins, the best-studied being Rho, Cdc42, and Rac. Rho is required for formation of focal adhesions and stress fibers, while constitutive Rac causes formation of plasma membrane extensions (Ridley and Hall, 1992; Ridley et al., 1992). Cdc42 activity promotes finger-like extrusions called filopodia (Nobes and Hall, 1995).
The tight junction is a key target of Salmonella, as infection with S. Typhimurium induces altered localization of ZO-2 and claudin-1, degradation of ZO-1, and promotes dephosphorylation of occludin in T84 cells (Kohler et al., 2007). Salmonella SPI-1 effectors SopB, SopE, SopE2, and SipA each play various roles in inducing ruffling and bacterial uptake by changing the localization and expression of ZO-1 and occludin, although SipA and SopB alone are not sufficient for this process (Boyle et al., 2006). These same effectors were also associated with altered epithelial cell polarity. Specifically, SopB (or SopB/SigD) is a phosphoinositide phosphatase that promotes formation of macropinosomes (Hernandez et al., 2004). This effector is capable of indirectly stimulating Cdc42-dependent cytoskeletal rearrangements (Zhou et al., 2001), which can lead to extension of the cell membrane around the bacterium. Similarly, SopE and SopE2 act like GEFs, and activate Cdc42 and Rac1 GTPases to induce cytoskeletal rearrangements that favor bacterial uptake (Stender et al., 2000). SipA and SipC associate with actin and promote bundling of actin filaments so as to facilitate macropinocytosis of Salmonella (McGhie et al., 2001).
As control mechanisms, Salmonella also secrete SptP and AvrA. SptP acts like a GAP, and essentially reverses the cytoskeletal rearrangements that occur during Salmonella uptake (Fu and Galan, 1999). AvrA, which stabilizes tight junctions (Liao et al., 2008) and inhibits NF-kB activation (Collier-Hyams et al., 2002), keeps the Salmonella-induced inflammation response in check.
Immune response
PAMP Receptors
Intestinal epithelial cells can recognize invading pathogens by capturing common viral or bacterial components, known as pathogen-associated molecular patterns (PAMPs). Toll-like receptors (TLRs) are a well-studied family of PAMP receptors. Several types of intestinal epithelial cells have been shown to express TLRs (Elphick and Mahida, 2005; Tyrer et al., 2006; Palazzo et al., 2007; Gribar et al., 2008), and each type of receptor recognizes a unique PAMP. TLR4 recognizes lipopolysaccharide (LPS) from Gram (−) bacterial cell walls, TLR2 in concert with TLR1 or TLR6 recognizes triacyl or diacyl bacterial lipopeptide, respectively, and TLR5 recognizes flagellin protein from bacterial flagella. The binding of a TLR to its cognate antigen triggers activation of a signaling cascade that ultimately, via NF-kB activation, induces production of pro-inflammatory cytokines. TLR5-flagellin interaction during S. Typhimurium in mouse infections was shown to regulate the early stages of Salmonella infection and immune response. Peyer's patches and mesenteric lymph nodes (MLN) in TLR5-deficient mice accumulate more Salmonella than wild-type littermates, and aflagellate Salmonella accumulate more in Peyer's patches and MLN of wild-type mice than do wild-type Salmonella (Fournier et al., 2009). As Salmonella encounter Peyer's patches after traversing the epithelial monolayer, regulation at this stage would be key to preventing further bacterial dissemination.
NLRs, or nucleotide-binding and oligomerization domain (NOD)-like receptors, are another established family of PAMP receptors that recognize components of bacterial peptidoglycan. The NLR family includes Nod1 and Nod2, both of which are expressed intracellularly by intestinal epithelial cells (MacDonald and Monteleone, 2005). Nod1 recognizes – d-glutamyl-meso-diaminopimelic (DAP) acid, expressed predominantly by Gram (−) bacteria (Chamaillard et al., 2003), while Nod2 recognizes muramyl dipeptide (MDP), which is common to Gram (−) and Gram (+) bacteria (Girardin et al., 2003). Binding between Nod1 and Nod2 and their specific PAMPs is followed by association with the adaptor protein Rip2, which initiates production of NF-kB-dependent pro-inflammatory cytokines. A recent paper suggests the role of Rip2-mediated inflammatory responses is key during Salmonella infection only when SPI-2 effectors are present (Geddes et al., 2010). As SPI-2 effectors are associated predominantly with intracellular survival, Rip2, and thus Nod1 and Nod2, may be required for dampening the ability of Salmonella to survive after invading host cells.
Neutrophil recruitment
The release of IL-8 resulting from pathogen-induced immune signaling stimulates the recruitment of neutrophils from blood vessels to the basolateral (serosal) surface (Figure 2). Neutrophils are phagocytic white blood cells that are among the first line of the innate immune defense in response to pathogens. Recruitment of neutrophils to the apical (mucosal) surface is mediated by release of hepoxilin A3 (HXA3), a potent neutrophil chemoattractant, and a metabolite of the arachidonic acid pathway (McCormick, 2007). SipA at the apical surface induces a lipid signal cascade that includes the activation of PKC, which causes the release of arachidonic acid from the plasma membrane (Wall et al., 2007). Arachidonic acid is then converted to HXA3 by 12-lipoxygenase activity. Release of HXA3 is facilitated by the ABC transporter, MRP2 (Pazos et al., 2008), and generates a concentration gradient across the tight junction and through the paracellular space. The recruitment of neutrophils superficially seems solely an act of host defense; however the rapid migration of these cells through the epithelial monolayer may actually loosen the epithelial cell–cell interactions, and thus create space through which Salmonella can invade, further facilitating PMN infiltration (Kohler et al., 2007). Indeed, PMN transmigration has been demonstrated to reduce epithelial monolayer resistance in vitro (Nash et al., 1987).
Salmonella Reside in the Salmonella Containing Vacuole
The Salmonella containing vacuole (SCV) is an intracellular vacuole that forms via macropinocytosis of Salmonella. The SopB effector helps direct the maturation of the SCV into a compartment suitable for bacterial survival and replication (Bakowski et al., 2008), and may function to steer the compartment away from the endocytic pathway (Hernandez et al., 2004). After formation of the SCV, many T3SS2 effectors assist in formation of Salmonella-induced filaments (Sifs), localization of the SCV, and intravacuolar replication.
SifA, SseF, SseG, and SopD2 are involved with Sif formation, which results in the extension of tubules outward from the SCV after fusion with late endosomal compartments (Brumell et al., 2001). SifA is required for Sif formation (Stein et al., 1996), as its absence precludes formation of any Sif structures. SseF and SseG are also required for proper Sif formation, as mutants lacking either of these effectors only form Sif-like structures that differ in composition from fully formed Sifs (Kuhle et al., 2004). SopD2 has recently been shown to balance the effects of SifA, as its loss in SifA mutants restores SCV stability in the presence of other effectors (Schroeder et al., 2010). The role of Sifs in Salmonella survival and replication has yet to be determined. To date, these structures have not been observed in vivo. SseF and SseG also localize with, and promote bundling of, microtubules in the cytoplasm and help position the SCV near the microtubule organizing center (MTOC), which is close to the Golgi network (Kuhle et al., 2004; Ramsden et al., 2007). Proximity to the Golgi network appears necessary for efficient bacterial replication within the SCV, as disruption of the Golgi network and loss of SseG diminish bacterial growth (Salcedo and Holden, 2003).
Once Salmonella enter the host epithelial cell, they seem to form two populations with distinct doubling rates. One population, referred to as “hyper-replicating,” doubles approximately every 20 min while the population as a whole doubles approximately every 95 min (Knodler et al., 2010). Interestingly, at least a third of the hyper-replicating population was found in the cytosol and express T3SS1 genes, while the slower replicating population was found in SCVs, and was expressing T3SS2 genes. These findings suggest that while Salmonella are capable of replicating within the SCV, cytosolic replication may be more efficient.
Salmonella Induces Ileal Secretion
One of the hallmarks of salmonellosis is a severe, watery diarrhea caused by high levels of fluid secretion into the intestines. Various T3SS1 effectors are proposed to mediate the level of fluid secretion into the gut, likely due to their ability to induce inflammation, including SopA, SopB, SopD, SopE2, and SipA (Zhang et al., 2002). Additionally, the loss of some genes encoded on SPI-5, including SopB, significantly reduced the level of S. dublin-induced fluid secretion from ligated ileal loops (Wood et al., 1998). One theory is that the rapid influx of neutrophils, in response to the inflammation caused by secreted effectors, impairs the epithelial barrier to an extent that results in leakage of extravascular fluids (Zhang et al., 2003). Neutrophil migration could also lead to chloride secretion by epithelial cells (Madara et al., 1993), an event that is compensated for by the subsequent secretion of water in an attempt by the host to restore ion balance.
Mechanisms of Salmonella Escape from Epithelial Cells and Dissemination
Salmonella Typhi, using the same initial invasion mechanisms as S. Typhimurium can disseminate in humans to cause systemic disease through infection of the gallbladder, liver, spleen, and bone marrow (Gonzalez-Escobedo et al., 2011). In mice, S. Typhimurium is capable of invading and destroying M cells, allowing penetration of the intestinal epithelium (Jones et al., 1994), and subsequent phagocytosis by macrophages. S. Typhimurium survival within the macrophages is mediated by SPI-2 effectors (Cirillo et al., 1998) and is essential for dissemination.
The role of the MLN in bacterial dissemination was closely explored by Voedisch et al. Here, the authors show that dendritic cells are major suppliers of S. Typhimurium to the MLN, but they alone are not regulating dissemination. After permitting re-circulation of intestinal lymph following removal of the MLN (mesenteric adenectomy), the authors observed increased liver colonization by S. Typhimurium in wild-type mice, and increased colonization of liver and spleen in NRAMP1+ 129Sv mice. Wild-type levels of liver and spleen colonization were observed in Rag2-deficient mice, suggesting the observations in the adenectomized mice were not artifacts of a weak adaptive immune response (Voedisch et al., 2009). These results suggest that while dendritic cells supply the MLN with S. Typhimurium, the MLN is the final barrier to dissemination in this pathway, as its presence is needed to limit the circularization of infected dendritic cells.
Although most NTS infections are self-limiting, NTS strains are capable of inducing prolonged infection, particularly in those with weakened immune systems. To cause prolonged infection, Salmonella must have a mechanism in place to facilitate escape from infected host cells. A recent paper suggests Salmonella escape their intracellular niche by co-opting the epithelial cell shedding process, a host mechanism used to remove dying epithelial cells. During this process, called extrusion, cells adjacent to a dying cell contract and force the dying cell out into the lumen (Madara, 1990; Mayhew et al., 1999). The authors found that during Salmonella infection, extrusion rates were increased, and about 10% of infected cells underwent extrusion, followed by inflammatory cell death. In contrast, less than 1% of uninfected cells underwent extrusion, and those that were extruded did not exhibit inflammatory cell death (Knodler et al., 2010). Escape into the lumen can permit Salmonella to infect additional cells, or to exit the host completely as part of its transmission cycle.
Concluding Remarks
Salmonella employ various mechanisms to overcome host defense mechanisms. By evolving ways to subvert, mimic, antagonize, or exploit a defense strategy, Salmonella maintain their ability to infect vertebrate hosts. The present and future research endeavors aimed at better understanding the tools Salmonella use to invade and traverse the mucosal intestinal epithelia will provide invaluable knowledge that will help devise ways to better treat and prevent Salmonella infections.
Statements
Acknowledgments
This work was supported by the National Institutes of Health Grant DK56754 and the Crohn's and Colitis Foundation of America to Beth A. McCormick.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
AberleH.BauerA.StappertJ.KispertA.KemlerR. (1997). β-catenin is a target for the ubiquitin–proteasome pathway. EMBO J.16, 3793–3804.
2
AbrahamsG. L.MullerP.HenselM. (2006). Functional dissection of SseF, a Type III Effector protein involved in positioning the Salmonella-containing vacuole. Traffic7, 950–965.10.1111/j.1600-0854.2006.00454.x
3
AdachiM.InokoA.HataM.FuruseK.UmedaK.ItohM.TsukitaS. (2006). Normal establishment of epithelial tight junctions in mice and cultured cells lacking expression of ZO-3, a tight-junction MAGUK protein. Mol. Cell. Biol.26, 9003–9015.10.1128/MCB.01811-05
4
AdamsC. L.ChenY.SmithS. J.NelsonW. J. (1998). Mechanisms of epithelial cell–cell adhesion and cell compaction revealed by high-resolution tracking of E-cadherin–green fluorescent protein. J. Cell Biol.142, 1105–1119.10.1083/jcb.142.4.1105
5
AndreevaA. Y.KrauseE.MüllerE. C.BlasigI. E.UtepbergenovD. I. (2001). Protein kinase C regulates the phosphorylation and cellular localization of occludin. J. Biol. Chem.42, 38480–38486.10.1074/jbc.M104923200
6
AyabeT.SatchellD. P.WilsonC. L.ParksW. C.SelstedM. E.OuelletteA. J. (2000). Secretion of microbicidal α-defensins by intestinal Paneth cells in response to bacteria. Nat. Immunol.1, 113–118.10.1038/77783
7
BackhedF.LeyR. E.SonnenburgJ. L.PetersonD. A.GordonJ. I. (2005). Host-bacterial mutualism in the human intestine. Science307, 1915–1920.10.1126/science.1104816
8
BakowskiM. A.BraunV.BrumellJ. H. (2008). Salmonella-containing vacuoles: directing traffic and nesting to grow. Traffic9, 2022–2031.10.1111/j.1600-0854.2008.00827.x
9
BarmanM.UnoldD.ShifleyK.AmirE.HungK.BosN.SalzmanN. (2008). Enteric salmonellosis disrupts the microbial ecology of the murine gastrointestinal tract. Infect. Immun.76, 907–915.10.1128/IAI.01432-07
10
Baus-LoncarM.SchmidJ.Lalaniel-N.RosewellI.GoodladR. A.StampG. W.BlinN.KayademirT. (2005). Trefoil factor 2 (Tff2) Deficiency in murine digestive track influences the immune system. Cell Physiol. Biochem. 16, 31–42.10.1159/000087729
11
BeuzonC. R.MéresseS.UnsworthK. E.Ruíz-AlbertJ.GarvisS.WatermanS. R.RyderT. A.BoucrotE.HoldenD. W. (2000). Salmonella maintains the integrity of its intracellular vacuole through the action of SifA. EMBO J. 19, 3235–3249.10.1093/emboj/19.13.3235
12
BoyleE. C.BrownN. F.FinlayB. B. (2006). Salmonella enterica serovar Typhimurium effectors SopB, SopE, SopE2 and SipA disrupt tight junction structure and function. Cell. Microbiol.8, 1946–1957.10.1111/j.1462-5822.2006.00762.x
13
BrawnL. C.HaywardR. D.KoronakisV. (2007). Salmonella SPI1 effector SipA persists after entry and cooperates with a SPI2 effector to regulate phagosome maturation and intracellular replication. Cell Host Microbe1, 63–75.10.1016/j.chom.2007.02.001
14
BrumellJ. H.TangP.MillsS. D.FinlayB. B. (2001). Characterization of Salmonella-induced filaments (Sifs) reveals a delayed interaction between Salmonella-containing vacuoles and late endocytic compartments. Traffic2, 643–653.10.1034/j.1600-0854.2001.20907.x
15
ChamaillardM.HashimotoM.HorieY.MasumotoJ.QiuS.SaabL.OguraY.KawasakiA.FukaseK.KusumotoS.ValvanoM. A.FosterS. J.MakT. W.NuñezG.InoharaN. (2003). An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol. 4, 702–707.10.1038/ni945
16
ChenY.StewartD. B.NelsonJ. W. (1999). Coupling Assembly of the E-Cadherin/β-Catenin complex to efficient endoplasmic reticulum exit and basal-lateral membrane targeting of E-Cadherin in polarized MDCK cells. J. Cell Biol.144, 687–699.10.1083/jcb.144.4.687
17
CirilloD. M.ValdiviaR. H.MonackD. M.FalkowS. (1998). Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival. Mol. Microbiol.30, 175–188.10.1046/j.1365-2958.1998.01048.x
18
ClarkM. A.HirstB. H.JepsonM. A. (1998). Inoculum composition and Salmonella pathogenicity island 1 regulate M-cell invasion and epithelial destruction by Salmonella typhimurium. Infect. Immun.66, 724–731.
19
ClarkM. A.ReedK. A.LodgeJ.StephenJ.HirstB. H.JepsonM. A. (1996). Invasion of murine intestinal M cells by Salmonella typhimurium inv mutants severely deficient for invasion of cultured cells. Infect. Immun.64, 4363–4368.
20
Collier-HyamsL. S.ZengH.SunJ.TomlinsonA. D.BaoZ. Q.ChenH.MadaraJ. L.OrthK.NeishA. S. (2002). Cutting edge: Salmonella AvrA effector inhibits the key proinflammatory, anti-apoptotic NF-kB pathway. J. Immunol.169, 2846–2850.
21
CroswellA.AmirE.TeggatzP.BarmanM.SalzmanN. H. (2009). Prolonged impact of antibiotics on intestinal microbial ecology and susceptibility to enteric Salmonella infection. Infect. Immun.77, 2741–2753.10.1128/IAI.00006-09
22
DavisM. A.IretonR. C.ReynoldsA. B. (2003). A core function for p120-catenin in cadherin turnover. J. Cell Biol.163, 525–534.10.1083/jcb.200307111
23
DeplanckeB.GaskinsH. R. (2001). Microbial modulation of innate defense: goblet cells and the intestinal mucus layer. Am. J. Clin. Nutr.73(Suppl.), 1131S–1141S.
24
DuanY.LiaoA. P.KuppireddiS.YeZ.CiancioM. J.SunJ. (2007). β-Catenin activity negatively regulates bacteria-induced inflammation. Lab. Invest.87, 613–624.
25
ElphickD. A.MahidaY. R. (2005). Paneth cells: their role in innate immunity and inflammatory disease. Gut54, 1802–1809.10.1136/gut.2005.068601
26
Etienne-MannevilleS.HallA. (2002). Rho GTPases in cell biology. Nature420, 629–635.10.1038/nature01148
27
FaucherS. P.PorwollikS.DozoisC. M.McClellandM.DaigleF. (2006). Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences. Proc. Natl. Acad. Sci. U.S.A.103, 1906–1911.10.1073/pnas.0509183103
28
FinlayB.RuschkowskiS.DedharS. (1991). Cytoskeletal rearrangements accompanying Salmonella entry into epithelial cells. J. Cell Sci.99, 283–296.
29
ForestC. G.FerraroE.SabbaghS. C.DaigleF. (2010). Intracellular survival of Salmonella enterica serovar Typhi in human macrophages is SPI-2 independent. Microbiology156(Pt 12), 3689–3698.10.1099/mic.0.041624-0
30
ForsterC. (2008). Tight junctions and the modulation of barrier function in disease. Histochem. Cell Biol.130, 55–70.10.1007/s00418-008-0424-9
31
FournierB.WilliamsI. R.GewirtzA. T.NeishA. S. (2009). Toll-like receptor 5-dependent regulation of inflammation in systemic Salmonella enterica serovar Typhimurium infection. Infect. Immun.77, 4121–4129.10.1128/IAI.00656-09
32
FreyA.GiannascaK. T.WeltzinR.GiannascaP. J.ReggioH.LencerW. I.NeutraM. R. (1996). Role of the glycocalyx in regulating access of microparticles to apical plasma membranes of intestinal epithelial cells: implications for microbial attachment and oral vaccine targeting. J. Exp. Med.184, 1045–1059.10.1084/jem.184.3.1045
33
FrostA. J.BlandA. P.WallisT. S. (1997). The early dynamic response of the calf ileal epithelium to Salmonella typhimurium. Vet. Pathol.34, 369–386.10.1177/030098589703400501
34
FuY.GalanJ. E. (1999). A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion. Nature401, 293–297.10.1038/45829
35
FuruseM.ItohM.HiraseT.NagafuchiA.YonemuraS.TsukitaS.TsukitaS. (1994). Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions. J. Cell Biol.127, 1617–1626.10.1083/jcb.127.6.1617
36
FuruseM.SasakiH.FujimotoK.TsukitaS. (1998). A single gene product, Claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts. J. Cell Biol.143, 391–401.10.1083/jcb.143.2.391
37
GalanJ. E. (1996). Molecular genetic bases of Salmonella entry into host cells. Mol. Microbiol.20, 263–271.10.1111/j.1365-2958.1996.tb02615.x
38
GeddesK.RubinoS.StreutkerC.ChoJ. H.MagalhaesJ. G.Le BourhisL.SelvananthamT.GirardinS. E.PhilpottD. J. (2010). Nod1 and Nod2 regulation of inflammation in the Salmonella colitis model. Infect. Immun.78, 5107–5115.10.1128/IAI.00759-10
39
GirardinS. E.BonecaI. G.VialaJ.ChamaillardM.LabigneA.ThomasG.PhilpottD. J.SansonettiP. J. (2003). Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J. Biol. Chem.278, 8869–8872.10.1074/jbc.C200651200
40
Gonzalez-EscobedoG.MarshallJ. M.GunnJ. S. (2011). Chronic and acute infection of the gall bladder by Salmonella Typhi: understanding the carrier state. Nat. Rev. Microbiol.9, 9–14.10.1038/nrmicro2490
41
Gonzalez-MariscalL.BetanzosA.Avila-FloresA. (2000). MAGUK proteins: structure and role in the tight junction. Cell Dev. Biol.11, 315–324.10.1006/scdb.2000.0178
42
GrahamS. M.MolyneuxE. M.WalshA. L.CheesbroughJ. S.MolyneuxM. E.HartC. A. (2000). Nontyphoidal Salmonella infections of children in tropical Africa. Pediatr. Infect. Dis. J.19, 1189–1196.10.1097/00006454-200012000-00016
43
GreenK. J.SimpsonC. L. (2007). Desmosomes: new perspectives on a classic. J. Investig. Dermatol.127, 2499–2515.
44
GribarS. C.AnandR. J.SodhiC. P.HackamD. J. (2008). The role of epithelial toll-like receptor signaling in the pathogenesis of intestinal inflammation. J. Leukoc. Biol.83, 493–498.10.1189/jlb.0607358
45
HapfelmeierS.EhrbarK.StecherB.BarthelM.KremerM.HardtW. D. (2004). Role of the Salmonella pathogenicity island 1 effector proteins SipA, SopB, SopE, and SopE2 in Salmonella enterica subspecies 1 Serovar Typhimurium colitis in streptomycin-pretreated mice. Infect. Immun.72, 795–809.10.1128/IAI.72.2.795-809.2004
46
HapfelmeierS.StecherB.BarthelM.KremerM.MüllerA. J.HeikenwalderM.StallmachT.HenselM.PfefferK.AkiraS.HardtW. D. (2005). The Salmonella pathogenicity island (SPI)-2 and SPI-1 Type III secretion systems allow Salmonella Serovar typhimurium to trigger colitis via MyD88-dependent and MYD88-independent mechanisms. J. Immunol.174, 1675–1685.
47
HardtW.ChenL. M.SchuebelK. E.BusteloX. R.GalánJ. E. (1998). S. typhimurium encodes an activator of Rho GTPases that induces membrane ruffling and nuclear responses in host cells. Cell93, 815–826.10.1016/S0092-8674(00)81442-7
48
HartsockA.NelsonJ. W. (2008). Adherens and tight junctions: structure, function and connections to the actin cytoskeleton. Biochim. Biophys. Acta1778, 660–669.10.1016/j.bbamem.2007.07.012
49
HashizumeT.TogawaA.NochiT.IgarashiO.KweonM. N.KiyonoH.YamamotoM. (2008). Peyer's patches are required for intestinal immunoglobulin a responses to Salmonella spp. Infect. Immun.76, 927–934.10.1128/IAI.01145-07
50
HaskinsJ.GuL.WittchenE. S.HibbardJ.StevensonB. R. (1998). ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin. J. Cell Biol.141, 199–208.10.1083/jcb.141.1.199
51
HatsellS.CowinP. (2001). Deconstructing desmoplakin. Nat. Cell Biol.3, E270–E272.10.1038/ncb1201-e270
52
HaywardR. D.McGhieE. J.KoronakisV. (2000). Membrane fusion activity of purified SipB, a Salmonella surface protein essential for mammalian cell invasion. Mol. Microbiol.37, 727–739.10.1046/j.1365-2958.2000.02027.x
53
HernandezL. D.HuefferK.WenkM. R.GalanJ. E. (2004). Salmonella modulates vesicular traffic by altering phosphoinositide metabolism. Science304, 1805–1807.10.1126/science.1098188
54
ItohM.MoritaK.TsukitaS. (1999). Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and α-catenin. J. Biochem. Chem.274, 5981–5986.
55
JensenV. B.HartyJ. T.JonesB. D. (1998). Interactions of the invasive pathogens Salmonella Typhimurium, Listeria monocytogenes, and Shigella flexneri with M cells and murine Peyer's patches. Infect. Immun.66, 3758–3766.
56
JiangX.RossaneseO. W.BrownN. F.Kujat-ChoyS.GalánJ. E.FinlayB. B.BrumellJ. H. (2004). The related effector proteins SopD and SopD2 from Salmonella enterica serovar Typhimurium contribute to virulence during systemic infection of mice. Mol. Microbiol.54, 1186–1198.10.1111/j.1365-2958.2004.04344.x
57
JonesB. D.GhoriN.FalkowS. (1994). Salmonella typhimurium initiates murine infection by penetrating and destroying the specialized epithelial M cells of the Peyer's patches. J. Exp. Med.180, 15–23.10.1084/jem.180.1.15
58
KanigaK.TuckerS.TrollingerD.GalanJ. E. (1995). Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella Typhimurium entry into cultured epithelial cells. J. Bacteriol.177, 3965–3971.
59
KnodlerL. A.Steele-MortimerO. (2005). The Salmonella effector PipB2 affects late endosome/lysosome distribution to mediate Sif extension. Mol. Biol. Cell16, 4108–4123.10.1091/mbc.E05-04-0367
60
KnodlerL. A.VallanceB. A.CelliJ.WinfreeS.HansenB.MonteroM.Steele-MortimerO. (2010). Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia. Proc. Natl. Acad. Sci. U.S.A.107, 17733–17738.10.1073/pnas.1006098107
61
KochP. J.FrankeW. W. (1994). Desmosomal cadherins: another growing multigene family of adhesion molecules. Curr. Opin. Cell Biol.6, 682–687.10.1016/0955-0674(94)90094-9
62
KohlerH.SakaguchiT.HurleyB. P.KaseB. A.ReineckerH. C.McCormickB. A. (2007). Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Am. J. Physiol. Gastroinest. Liver Physiol.293, G178–G187.10.1152/ajpgi.00535.2006
63
KraehenbuhlJ.NeutraM. R. (2000). Epithelial M cells: differentiation and function. Annu. Rev. Cell Dev. Biol.16, 301–332.10.1146/annurev.cellbio.16.1.301
64
KuhleV.JackelD.HenselM. (2004). Effector proteins encoded by Salmonella pathogenicity island 2 interfere with the microtubule cytoskeleton after translocation into host cells. Traffic5, 356–370.10.1111/j.1398-9219.2004.00179.x
65
Lara-TejeroM.GalanJ. E. (2009). Salmonella enterica Serovar Typhimurium pathogenicity island 1-encoded type III secretion system translocases mediate intimate attachment to nonphagocytic cells. Infect. Immun.77, 2635–2642.10.1128/IAI.00077-09
66
LawleyT. D.ChanK.ThompsonL. J.KimC. C.GovoniG. R.MonackD. M. (2006). Genome-wide screen for Salmonella genes required for long-term systemic infection of the mouse. PLoS Pathogens2, e11.10.1371/journal.ppat.0020011
67
LawleyT. D.BouleyD. M.HoyY. E.GerkeC.RelmanD. A.MonackD. M. (2008). Host transmission of Salmonella enterica Serovar typhimurium is controlled by virulence factors and indigenous intestinal microbiota. Infect. Immun.76, 403–416.10.1128/IAI.01189-07
68
LeLouardH.HenriS.De BovisB.MugnierB.Chollat-NamyA.MalissenB.MéresseS.GorvelJ. P. (2010). Pathogenic bacteria and dead cells are internalized by a unique subset of Peyer's Patch dendritic cells that express lysozyme. Gastroenterology138, 173–184.10.1053/j.gastro.2009.09.051
69
LesnickM. L.ReinerN. E.FiererJ.GuineyD. G. (2001). The Salmonella spvB virulence gene encodes an enzyme that ADP-ribosylates actin and destabilizes the cytoskeleton of eukaryotic cells. Mol. Microbiol.39, 1464–1470.10.1046/j.1365-2958.2001.02360.x
70
LiaoA. P.PetrofE. O.KuppireddiS.ZhaoY.XiaY.ClaudE. C.SunJ. (2008). Salmonella Type III effector AvrA stabilizes cell tight junctions to inhibit inflammation in intestinal epithelial cells. PLOS ONE3, e2369.10.1371/journal.pone.0002369
71
LibbyS. J.BrehmM. A.GreinerD. L.ShultzL. D.McClellandM.SmithK. D.CooksonB. T.KarlinseyJ. E.KinkelT. L.PorwollikS.CanalsR.CummingsL. A.FangF. C. (2010). Humanized nonobese diabetic-scid IL2rynull mice are susceptible to lethal Salmonella Typhi infection. Proc. Natl. Acad. Sci. U.S.A.107, 15589–15594.10.1073/pnas.1005566107
72
MacDonaldT. T.MonteleoneG. (2005). Immunity, inflammation, and allergy in the gut. Science307, 1920–1925.10.1126/science.1106442
73
MadaraJ. L. (1990). Maintenance of the macromolecular barrier at cell extrusion sites in intestinal epithelium: physiological rearrangement of tight junctions. J. Membr. Biol.116, 177–184.10.1007/BF01868675
74
MadaraJ. L.PatapoffT. W.Gillece-CastroB.ColganS. P.ParkosC. A.DelpC.MrsnyR. J. (1993). 5’-Adenosine monophosphate is the neutrophil-derived paracrine factor that elicits chloride secretion from T84 intestinal epithelial cell monolayers. J. Clin. Invest.91, 2320–2325.10.1172/JCI116462
75
Martinez-ArgudoI.JepsonM. A. (2008). Salmonella translocates across an in vitro M cell model independently of SPI-1 and SPI-2. Microbiology154, 3887–3894.10.1099/mic.0.2008/021162-0
76
Martinez-PalomoA.ErlijD. (1975). Structure of tight junctions in epithelia with different permeability. Proc. Natl. Acad. Sci. U.S.A.72, 4487–4491.10.1073/pnas.72.11.4487
77
MayhewT. M.MyklebustR.WhybrowA.JenkinsR. (1999). Epithelial integrity, cell death and cell loss in mammalian small intestine. Histol. Histolpathol.14, 257–267.
78
MazmanianS. K.LiuC. H.TzianabosA. O.KasperD. L. (2005). An immunodeficiency molecule of symbiotic bacteria directs maturation of the host immune system. Cell122, 107–118.10.1016/j.cell.2005.05.007
79
MazurkiewiczP.ThomasJ.ThompsonJ. A.LiuM.ArbibeL.SansonettiP.HoldenD. W. (2008). SpvC is a Salmonella effector with phosphothreonine lyase activity on host mitogen-activated protein kinases. Mol. Microbiol.67, 1371–1383.10.1111/j.1365-2958.2008.06134.x
80
McCormickB. A. (2007). Bacterial-induced hepoxilin A3 secretion as a pro-inflammatory mediator. FEBS J.274, 3513–3518.10.1111/j.1742-4658.2007.05911.x
81
McCormickB. A.StockerB. A. D.LauxD. C.CohenP. S. (1988). Roles of motility, chemotaxis, and penetration through and growth in intestinal mucus in the ability of an avirulent strain of Salmonella typhimurium to colonize the large intestine of streptomycin-treated mice. Infect. Immun.56, 2209–2217.
82
McGhieE. J.HaywardR. D.KoronakisV. (2001). Cooperation between actin-binding proteins of invasive Salmonella: SipA potentiates SipC nucleation and bundling of actin. EMBO J.20, 2131–2139.10.1093/emboj/20.9.2131
83
MeyerholzD. K.StabelT. J. (2003). Comparison of early ileal invasion by Salmonella enterica serovars choleraesuis and typhimurium. Vet. Pathol.40, 371–375.10.1354/vp.40-4-371
84
MeyerholzD. K.StabelT. J.AckermannM. R.CarlsonS. A.JonesB. D.PohlenzJ. (2002). Early epithelial invasion by Salmonella enterica serovar typhimurium DT104 in the swine ileum. Vet. Pathol.39, 712–720.10.1354/vp.39-6-712
85
NashS.StaffordJ.MadaraJ. L. (1987). Effects of Polymorphonuclear leukocyte transmigration on the barrier function of cultured intestinal epithelial monolayers. J. Clin. Invest.80, 1104–1113.10.1172/JCI113167
86
NevolaJ. J.LauxD. C.CohenP. S. (1987). In vivo colonization of the mouse large intestine and in vitro penetration of intestinal mucus by an avirulent smooth strain of Salmonella typhimurium and its lipopolysaccharide-deficient mutant. Infect. Immun.55, 2884–2890.
87
NobesC. D.HallA. (1995). Rho, Rac, and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell81, 53–62.10.1016/0092-8674(95)90370-4
88
NorrisF. A.WilsonM. P.WallisT. S.GalyovE. E.MajerusP. W. (1998). SopB, a protein required for virulence of Salmonella dublin, is an inositol phosphate phosphatase. Proc. Natl. Acad. Sci. U.S.A.95, 14057–14059.10.1073/pnas.95.24.14057
89
PalazzoM.BalsariA.RossiniA.SelleriS.CalcaterraC.GariboldiS.ZanobbioL.ArnaboldiF.ShiraiY. F.SerraoG.RumioC. (2007). Activation of enteroendocrine cells via TLRs induces hormone, chemokine, and defensin secretion. J. Immunology178, 4296–4303.
90
PazosM.SiccardiD.MumyK. L.BienJ. D.LouieS.ShiH. N.GronertK.MrsnyR. J.McCormickB. A. (2008). Multidrug resistance-associated transporter 2 regulates mucosal inflammation by facilitating the synthesis of hepoxilin A3. J. Immunol.181, 8044–8052.
91
PlayfordR. J.MarchbankT.GoodladR. A.ChineryR. A.PoulsomR.HanbyA. M. (1996). Transgenic mice that overexpress the human trefoil factor peptide pS2 have an increased resistance to intestinal damage. Proc. Natl. Acad. Sci. U.S.A.93, 2137–2142.10.1073/pnas.93.5.2137
92
PohJ.OdendallC.SpanosA.BoyleC.LiuM.FreemontP.HoldenD. W. (2008). SteC is a Salmonella kinase required for SPI-2-dependent F-actin remodeling. Cell. Microbiol.10, 20–30.
93
QuezadaC. M.HicksS. W.GalanJ. E.StebbinsC. E. (2009). A family of Salmonella virulence factors functions as a distinct class of autoregulated E3 ubiquitin ligases. Proc. Natl. Acad. Sci. U.S.A.106, 4864–4869.10.1073/pnas.0811058106
94
RadtkeA.WilsonJ. W.SarkerS.NickersonC. A. (2010). Analysis of interactions of Salmonella type three secretion mutants with 3-D intestinal epithelial cells. PLoS ONE5, e15750.10.1371/journal.pone.0015750
95
RaffatelluM.WilsonR. P.ChessaD.Andrews-PolymenisH.TranQ. T.LawhonS.KhareS.AdamsL. G.BäumlerA. J. (2005). SipA, SopA, SopB, SopD, and SopE2 contribute to Salmonella enterica serotype typhimurium invasion of epithelial cells. Infect. Immun.73, 146–154.10.1128/IAI.73.1.146-154.2005
96
Rakoff-NahoumS.PaglinoJ.Eslami-VarzanehF.EdbergS.MedzhitovR. (2004). Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis. Cell118, 229–241.10.1016/j.cell.2004.07.002
97
RamsdenA. E.MotaL. J.MünterS.ShorteS. L.HoldenD. W. (2007). The SPI-2 type III secretion system restricts motility of Salmonella-containing vacuoles. Cell. Microbiol.9, 2517–2529.10.1111/j.1462-5822.2007.00977.x
98
RidleyA. J.HallA. (1992). The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell70, 389–399.10.1016/0092-8674(92)90163-7
99
RidleyA. J.PatersonH. F.JohnstonC. L.DiekmannD.HallA. (1992). The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell70, 401–410.10.1016/0092-8674(92)90164-8
100
Ruiz-AlbertJ.YuX. J.BeuzónC. R.BlakeyA. N.GalyovE. E.HoldenD. W. (2002). Complementary activities of SseJ and SifA regulate dynamics of the Salmonella typhimurium vacuolar membrane. Mol. Microbiol.44, 645–661.10.1046/j.1365-2958.2002.02912.x
101
RytkonenA.HoldenD. W. (2007). Bacterial interference of ubiquitination and deubiquitination. Cell Host Microbe1, 13–22.10.1016/j.chom.2007.02.003
102
RytkonenA.PohJ.GarmendiaJ.BoyleC.ThompsonA.LiuM.FreemontP.HintonJ. C. D.HoldenD. W. (2007). SseL, a Salmonella deubiquitinase required for macrophage killing and virulence. Proc. Natl. Acad. Sci. U.S.A.104, 3502–3507.10.1073/pnas.0610095104
103
SabbaghS. C.ForestC. G.LepageC.LeclercJ. M.DaigleF. (2010). So similar, yet so different: uncovering distinctive features in the genomes of Salmonella enterica serovars Typhimurium and Typhi. FEMS Microbiol.305, 1–13.10.1111/j.1574-6968.2010.01904.x
104
SalcedoS. P.HoldenD. W. (2003). SseG, a virulence protein that targets Salmonella to the golgi network. EMBO J.22, 5003–5014.10.1093/emboj/cdg517
105
SalzmanN. H.ChouM. M.de JongH.LiuL.PorterE. M.PatersonY. (2003). Enteric Salmonella infection inhibits paneth cell antimicrobial peptide expression. Infect. Immun.71, 1109–1115.10.1128/IAI.71.3.1109-1115.2003
106
SchroederN.HenryT.de ChastellierC.ZhaoW.GuilhonA. A.GorvelJ. P.MéresseS. (2010). The virulence protein SopD2 regulates membrane dynamics of Salmonella-containing vacuoles. PLOS Pathogens6, e1001002.10.1371/journal.ppat.1001002
107
SrikanthC. V.WallD. M.Maldonado-ContrerasA.ShiH. N.ZhouD.DemmaZ.MumyK. L.McCormickB. A. (2010). Salmonella pathogenesis and processing of secreted effectors by caspase-3. Science330, 390–393.10.1126/science.1194598
108
StebbinsC. E.GalanJ. E. (2000). Modulation of host signaling by a bacterial mimic: structure of the Salmonella effector SptP bound to Rac1. Mol. Cell6, 1449–1460.10.1016/S1097-2765(00)00141-6
109
StebbinsC. E.GalanJ. E. (2003). Priming virulence factors for delivery into the host. Nat. Rev. Mol. Cell Biol.4, 738–744.10.1038/nrm1201
110
StecherB.RobbianiR.WalkerA. W.WestendorfA. M.BarthelM.KremerM.ChaffronS.MacphersonA. J.BuerJ.ParkhillJ.DouganG.von MeringC.HardtW. D. (2007). Salmonella enterica serovar typhimurium exploits inflammation to compete with the intestinal microbiota. PLOS Biol.5, 2177–2189.10.1371/journal.pbio.0050244
111
SteinM.LeungK. Y.ZwickM.Garcia-del PortilloF.FinlayB. B. (1996). Identification of a Salmonella virulence gene required for formation of filamentous structures containing lysosomal membrane glycoproteins within epithelial cells. Mol. Microbiol.20, 151–164.10.1111/j.1365-2958.1996.tb02497.x
112
StenderS.FriebelA.LinderS.RohdeM.MiroldS.HardtW. D. (2000). Identification of SopE2 from Salmonella typhimurium, a conserved guanine nucleotide exchange factor for Cdc42 of the host cell. Mol. Microbiol.36, 1206–1221.10.1046/j.1365-2958.2000.01933.x
113
TakeichiM. (1991). Cadherin cell adhesion receptors as a morphogenetic regulator. Science251, 1451–1455.10.1126/science.2006419
114
TamM. A.RydstromA.SundquistM.WickM. J. (2008). Early cellular responses to Salmonella infection: dendritic cells, monocytes, and more. Immunol. Rev.225, 140–162.10.1111/j.1600-065X.2008.00679.x
115
TsukitaS.KatsunoT.YamazakiY.UmedaK.TamuraA.TsukitaS. (2009). Roles of ZO-1 and ZO-2 in establishment of the belt-like adherens and tight junctions with paracellular permselective barrier function. Ann. N.Y. Acad. Sci.1165, 44–52.10.1111/j.1749-6632.2009.04056.x
116
TurnerJ. R. (2009). Intestinal mucosal barrier function in health and disease. Nat. Rev. Immunol.9, 799–809.10.1038/nri2653
117
TyrerP.FoxwellA. R.CrippsA. W.ApicellaM. A.KydJ. M. (2006). Microbial pattern recognition receptors mediate M-cell uptake of a gram-negative bacterium. Infect. Immun.74, 625–631.10.1128/IAI.74.1.625-631.2006
118
UmedaK.IkenouchiJ.Katahira-TayamaS.FuruseK.SasakiH.NakayamaM.MatsuiT.TsukitaS.FuruseM.TsukitaS. (2006). ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation. Cell126, 741–754.10.1016/j.cell.2006.06.043
119
VimalD. B.KhullarM.GuptaS.GangulyN. K. (2000). Intestinal mucins: the bindings sites for Salmonella Typhimurium. Mol. Cell. Biochem.204, 107–117.10.1023/A:1007015312036
120
VoedischS.KoeneckeC.DavidS.HerbrandH.FörsterR.RhenM.PabstO. (2009). Mesenteric lymph nodes confine DC-mediated dissemination of Salmonella and limit systemic disease in mice. Infect. Immun.77, 3170–3180.10.1128/IAI.00272-09
121
WallD. M.NadeauW. J.PazosM. A.ShiH. N.GalyovE. E.McCormickB. A. (2007). Identification of the Salmonella enterica serotype Typhimurium SipA domain responsible for inducing neutrophil recruitment across the intestinal epithelium. Cell. Microbiol.9, 2299–2313.10.1111/j.1462-5822.2007.00960.x
122
WoodM. W.JonesM. A.WatsonP. R.HedgesS.WallisT. S.GalyovE. E. (1998). Identification of a pathogenicity island required for Salmonella enteropathogenicity. Mol. Microbiol.29, 883–891.10.1046/j.1365-2958.1998.00984.x
123
WoodM. W.JonesM. A.WatsonP. R.SiberA. M.McCormickB. A.HedgesS.RosqvistR.WallisT. S.GalyovE. E. (2000). The secreted effector protein of Salmonella dublin, SopA, is translocated into eukaryotic cells and influences the induction of enteritis. Cell. Microbiol.2, 293–303.10.1046/j.1462-5822.2000.00054.x
124
YeZ.PetrofE. O.BooneD.ClaudE. C.SunJ. (2007). Salmonella effector AvrA regulation of colonic epithelial cell inflammation by deubiquitination. Am. J. Pathol.171, 882–892.10.2353/ajpath.2007.070220
125
YuX.Ruiz-AlbertJ.UnsworthK. E.GarvisS.LiuM.HoldenD. W. (2002). SpiC is required for secretion of Salmonella pathogenicity island 2 type III secretion system proteins. Cell. Microbiol.4, 531–540.10.1046/j.1462-5822.2002.00211.x
126
ZhangS.KingsleyR. A.SantosR. L.Andrews-PolymenisH.RaffatelluM.FigueiredoJ.NunesJ.TsolisR. M.AdamsL. G.BäumlerA. J. (2003). Molecular pathogenesis of Salmonella enterica serotype typhimurium-induced diarrhea. Infect. Immun.71, 1–12.10.1128/IAI.71.1.1-12.2003
127
ZhangS.SantosR. L.TsolisR. M.StenderS.HardtW. D.BäumlerA. J.AdamsL. G. (2002). The Salmonella enterica serotype typhimurium effector proteins SipA, SopA, SopB, SopD, and SopE2 Act in concert to induce diarrhea in calves. Infect. Immun.70, 3843–3855.10.1128/IAI.70.7.3843-3855.2002
128
ZhangY.HigashideW. M.McCormickB. A.ChenJ.ZhouD. (2006). The inflammation-associated Salmonella SopA is a HECT-like E3 ubiquitin ligase. Mol. Microbiol.62, 786–793.10.1111/j.1365-2958.2006.05407.x
129
ZhouD.ChenL. M.HernandezL.ShearsS. B.GalánJ. E. (2001). A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization. Mol. Microbiol.39, 248–259.10.1046/j.1365-2958.2001.02230.x
130
ZhouD.MoosekerM. S.GalánJ. E. (1999). Role of the S. Typhimurium actin-binding protein SipA in bacterial internalization. Science283, 2092–2095.10.1126/science.283.5410.2092
Summary
Keywords
Salmonella, intestinal mucosa, tight junctions
Citation
Hallstrom K and McCormick BA (2011) Salmonella Interaction with and Passage through the Intestinal Mucosa: Through the Lens of the Organism. Front. Microbio. 2:88. doi: 10.3389/fmicb.2011.00088
Received
01 February 2011
Accepted
13 April 2011
Published
29 April 2011
Volume
2 - 2011
Edited by
John S. Gunn, The Ohio State University, USA
Reviewed by
Brian K. Coombes, McMaster University, Canada; Leigh A. Knodler, NIAID, NIH, USA
Copyright
© 2011 Hallstrom and McCormick.
This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.
*Correspondence: Beth A. McCormick, Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA.e-mail: beth.mccormick@umassmed.edu
This article was submitted to Frontiers in Cellular and Infection Microbiology, a specialty of Frontiers in Microbiology.
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