It has come to our attention that in Figure 4B of the original article, we stated that the S. schleferi autoinducing peptide has the sequence YPFCIAYF. This peptide was synthesized, tested and found to have inhibitory activity. However, the correct AIP sequence is YPFCIGYF. We have now synthesized this peptide with the protocol stated below, which differs from the one published, and have tested the correct peptide for activity. As demonstrated below, we find that the new peptide has strong agr inhibitory activity, as expected, and, therefore, the conclusions of the paper remain the same.
Figure 4
Materials and methods
Chemical synthesis of S. schleiferi AIP
The S. schleiferi AIP was synthesized applying a strategy based on the C-terminal peptide N-acyl-benzimidazolinone (Nbz) derivative (Blanco-Canosa and Dawson, 2008), which was previously reported by Blackwell and coworkers (Tal-Gan et al., 2016) for AIP syntheses. Briefly, the linear peptide (20.0 μmol) was synthesized on 3-(Fmoc-amino)-4-aminobenzoyl aminomethyl polystyrene resin (Dawson Dbz AM resin; 0.49 mmol/g, Merck) by automated Fmoc solid-phase peptide synthesis (SPPS) as described in the Materials and Methods of the manuscript. The peptide (5.00 μmol) was then cyclized by Nbz formation, TFA-mediated cleavage from the resin, and stirring in aqueous guanidinium chloride (6 M in 0.1 M phosphate buffer, pH = 6.8)–MeCN (6:4) for 2 h at 50°C (Tal-Gan et al., 2016). Cyclization was followed by purification as described in our manuscript to give the title compound as a white fluffy solid (1.9 mg, 28%).
Purity: >98% as determined by UPLC–MS analysis at 230 nm.
MS: m/z calcd for C52H62N8O10S 991.4. ESI-MS found 991.4 [M+H+]. MALDI-TOF MS found 991.5 [M+H+].
β-lactamase assay and inhibitory concentration (IC50)
The method used is described by Nielsen et al. (2014). Briefly, the RN10829 (P2-agrA:P3-blaZ)/pagrC-I (WT) and RN10829(P2-agrA:P3-blaZ)/pagrC-I-R23H (AgrC const.) reporter strains were grown to an OD600 of 0.4–0.5 where a 1/10 volume of AIP-I containing supernatant (obtained from strain 8325-4) and 1/10 S. schleiferi supernatants were added to the reporter strain culture. In assays using heterologously expressed AIPSs 1/20 volume of AIP-I containing supernatant was challenged with 1/5 volume supernatant from expression cultures. Samples obtained at 30 min time intervals after addition of test solutions were analyzed for β-lactamase activity by nitrocefin conversion. The IC50 of the selected S. schleiferi supernatants was also tested using the β-lactamase assay, where a 1/10 volume (0.5 mL) of supernatant was added to the total volume of 5 mL of the reporter strain culture (RN10829-WT) representing the undiluted supernatant (100%). Then, 80, 60, 40, 20, 10, 5, 2.5 and 2% of the initial volume of the selected supernatant was added to obtain the IC50 curve. Statistical analysis was performed using the Student's t-test (2-tailed).
Results
S. schleiferi inhibition of S. aureus agr is AIP-mediated
To support that the S. schleiferi AIP that is responsible for inhibition of S. aureus RNAIII via AgrC agonist activity, we synthesized the S. schleiferi AIP with the sequence YPFCIGYF and tested the synthetic compound in the P3-blaZ reporter strain. Our results in this amended Figure 4B show that the S. schleiferi AIP is a potent inhibitor of S. aureus RNAIII expression and that it acts antagonistically on the reporter strain at low nanomolar concentrations.
Conflict of interest statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Statements
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
Blanco-CanosaJ. B.DawsonP. E. (2008). An efficient Fmoc-SPPS approach for the generation of thioester peptide precursors for use in native chemical ligation. Angew. Chem. Int. Ed.47, 6851–6855. 10.1002/anie.200705471
2
NielsenA.MånssonM.BojerM. S.GramL.LarsenT. O.NovickR. P.et al. (2014). Solonamide B Inhibits Quorum Sensing and Reduces Staphylococcus aureus Mediated Killing of Human Neutrophils. PLoS ONE9:e84992. 10.1371/journal.pone.0084992
3
Tal-GanY.IvancicM.CornilescubG.BlackwellH. E. (2016). Characterization of structural elements in native autoinducing peptides and non-native analogues that permit the differential modulation of AgrC-type quorum sensing receptors in Staphylococcus aureus. Org. Biomol. Chem.14, 113–121. 10.1039/c5ob01735a
Summary
Keywords
Staphylococcus aureus, Staphylococcus schleiferi, quorum sensing, agr, quorum sensing inhibition, auto-inducing peptide, cross-talk, anti-virulence therapy
Citation
Canovas J, Baldry M, Bojer MS, Andersen PS, Grzeskowiak PK, Stegger M, Damborg P, Olsen CA and Ingmer H (2017) Corrigendum: Cross-Talk between Staphylococcus aureus and Other Staphylococcal Species via the agr Quorum Sensing System. Front. Microbiol. 8:1075. doi: 10.3389/fmicb.2017.01075
Received
10 April 2017
Accepted
29 May 2017
Published
08 June 2017
Volume
8 - 2017
Edited by
Susanne Fetzner, Universität Münster, Germany
Reviewed by
Susanne Fetzner, Universität Münster, Germany; Mattias Collin, Lund University, Sweden
Updates
Copyright
© 2017 Canovas, Baldry, Bojer, Andersen, Grzeskowiak, Stegger, Damborg, Olsen and Ingmer.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Hanne Ingmer hi@sund.ku.dk
†These authors have contributed equally to this work.
This article was submitted to Systems Microbiology, a section of the journal Frontiers in Microbiology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.