In the original article, there was an error. We stated that “A similar phenotype was not achieved by deleting the genes coding for muramyl endopeptidase MepA, PbpG, NlpC, YedA, or YhdO”. This is incorrect as the correct names for “YedA” is “YebA” and the correct name for “YhdO” is “YdhO.”
A correction has been made to the Abstract:
“The impermeability barrier provided by the outer membrane of enteric bacteria, a feature lacking in Gram-positive bacteria, plays a major role in maintaining resistance to numerous antimicrobial compounds and antibiotics. Here we demonstrate that mutational inactivation of spr, coding for a muramyl endopeptidase, significantly sensitizes Salmonella enterica serovar Typhimurium to vancomycin without any accompanying apparent growth defect or outer membrane destabilization. A similar phenotype was not achieved by deleting the genes coding for muramyl endopeptidases MepA, PbpG, NlpC, YebA, or YdhO. The spr mutant showed increased autolytic behavior in response to not only vancomycin, but also to penicillin G, an antibiotic for which the mutant displayed a wild-type MIC. A screen for suppressor mutations of the spr mutant phenotype revealed that deletion of tsp (prc), encoding a periplasmic carboxypeptidase involved in processing Spr and PBP3, restored intrinsic resistance to vancomycin and reversed the autolytic phenotype of the spr mutant. Our data suggest that Spr contributes to intrinsic antibiotic resistance in S. Typhimurium without directly affecting the outer membrane permeability barrier. Furthermore, our data suggests that compounds targeting specific cell wall endopeptidases might have the potential to expand the activity spectrum of traditional Gram-positive antibiotics.”
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way.
Summary
Keywords
vancomycin, antibiotic resistance, Spr, MepS, YebA, MepM, Tsp, Prc
Citation
Vestö K, Huseby DL, Snygg I, Wang H, Hughes D and Rhen M (2019) Corrigendum: Muramyl Endopeptidase Spr Contributes to Intrinsic Vancomycin Resistance in Salmonella enterica Serovar Typhimurium. Front. Microbiol. 10:386. doi: 10.3389/fmicb.2019.00386
Received
29 January 2019
Accepted
13 February 2019
Published
05 March 2019
Volume
10 - 2019
Edited and reviewed by
Kunihiko Nishino, Osaka University, Japan
Updates
Copyright
© 2019 Vestö, Huseby, Snygg, Wang, Hughes and Rhen.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Kim Vestö Kim.Vesto@ki.se
This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology
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