Corrigendum: Changes in the Carbon Metabolism of Escherichia coli During the Evolution of Doxycycline Resistance

In the original article, there was an error. The methods used to extract and purify RNA were not well described.

A correction has been made to Materials and Methods, Transcriptome Sequencing and Analysis ofEscherichia coli section, paragraph 1:

“Total RNA of Escherichia coli was extracted using a E.Z.N.A Bacterial RNA Kit (R6950-01, OMEGA, USA) according to the manufacturer's instruction. A total of 3 μg of RNA per sample was used as input material for RNA sample preparation. Sequencing libraries were generated using the NEBNext^® Ultra™ Directional RNA Library Prep Kit for Illumina^® (NEB, USA) according to the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, MRZB12424) was used for removal of rRNA from total RNA preparations. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5×). First strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNaseH-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. In the reaction buffer, dNTPs with dTTP were replaced by dUTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3′ ends of DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare the samples for hybridization. To preferentially select cDNA fragments that were 150–200 bp in length, the library fragments were purified with the AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 μl of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min, followed by 5 min at 95°C before PCR. Then, PCR was performed with Phusion high-fidelity DNA polymerase, universal PCR primers, and Index (X) primer. Finally, products were purified (AMPure XP system), and library quality was assessed on am Agilent Bioanalyzer 2100 system.”

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Summary

Keywords

carbon metabolism, evolution, antibiotic resistance, DOX, Escherichia coli

Citation

Yang Y, Mi J, Liang J, Liao X, Ma B, Zou Y, Wang Y, Liang J and Wu Y (2022) Corrigendum: Changes in the Carbon Metabolism of Escherichia coli During the Evolution of Doxycycline Resistance. Front. Microbiol. 13:852577. doi: 10.3389/fmicb.2022.852577

Received

11 January 2022

Accepted

13 January 2022

Published

08 February 2022

Volume

13 - 2022

Edited by

Rustam Aminov, University of Aberdeen, United Kingdom

Reviewed by

Eric Altermann, AgResearch Ltd, New Zealand

Updates

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Copyright

© 2022 Yang, Mi, Liang, Liao, Ma, Zou, Wang, Liang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Yinbao Wu wuyinbao@scau.edu.cn

This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology

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