In the published article, there was an error in Table 1 as published. The sequence of the LAMP internal backward and forward primers (BIP and FIP, respectively) in Table 1 was displayed as BIP 5′ – TTG TGC TCG AGT CGT GGC GCA TAC GAT AGG TGC GAA T-3′ and FIP 5′ ACC GCA GCA TCT CAA TCA AGT CGA AGG CTC CGT CCT AAT – 3′, while the corrected sequences are BIP 5′- AGA AGT GTT TAA CTT GAT GAA GGG GAA ACA AAA CCG AAA CCA CTA−3′and FIP 5′- GCC AAG TAG AGA CTA CAA ACA TCT TTG GGT AAG GTA GAA AAT GTT GT−3′. The correct sequences were added to Table 1 and its caption remains the same as the original table, which appear below.
Table 1
| Type | Amplicon | Target | Assay | Sequence | References |
|---|---|---|---|---|---|
| Forward primer | 121 bp | S. mansoni repetitive region Sm1-7 (GenBank: M61098) | PCR and PCR-ELISA | 5-Biosg/GAT CTG AAT CCG ACC AAC CG−3′ | Gomes et al., 2009 |
| Reverse primer | 5′- ATA TTA ACG CCC ACG CTC TC- 3′ | ||||
| Probe | PCR-ELISA | 5-6[FAM]/TGG TTT CGG AGA TAC AAC GA-3′ | |||
| Forward primer | 120 bp | Human β-actin gene (GenBank: AY582799.1) | 5-Biosg/ACC TCA TGA AGA TCC TCA CC-3′ | Musso et al., 1996 | |
| Reverse primer | 5′- CCA TCT CTT GCT CGA AGT CC-3′ | ||||
| Probe | 5-6[FAM]/TCT CCT TAA TGC ACG CAC G-3′ | Gomes et al., 2010 | |||
| Forward primer | 90 bp | S. mansoni repetitive region Sm1-7 (GenBank: M61098) | qPCR | 5′-CCG ACC AAC CGT TCT ATG A-3′ | Siqueira et al., 2021 |
| Reverse primer | 5′-CAC GCT CTC GCA AAT AAT CTA AA-3′ | ||||
| Probe | 5′-6[FAM]/TCG TTG TAT CTC CGA AACCAC TGG ACG/[3BHQ1] | ||||
| Forward primer | 92 bp | Human β-actin gene (GenBank: AY582799.1) | 5′-CCA TCT ACG AGG GGT ATG-3′ | ||
| Reverse primer | 3′-GGT GAG GAT CTT CAT GAG GTA-5′ | ||||
| Probe | 5′- 6[JOE]/CCT GCG TCT GGA CCT GGC TG/[3BHQ1] | ||||
| Internal forward primer-FIP | NA | Mitochondrial S. mansoni minisatellite DNA region (GenBank: L27240) | LAMP | 5′- GCC AAG TAG AGA CTA CAA ACA TCT TTG GGT AAG GTA GAA AAT GTT GT−3′ | Fernández-Soto et al., 2014 |
| Internal backward primer-BIP | 5′- AGA AGT GTT TAA CTT GAT GAA GGG GAA ACA AAA CCG AAA CCA CTA−3′ | ||||
| External forward primer-F | 203 bp | 5′- TTA TCG TCT ATA GTA CGG TAG G-3′ | |||
| External backward primer-B3 | 5′- ATA CTT TAA CCC CCA CCA A-3′ |
Primers and probes used in the molecular tests.
bp, base pair; FAM, 6-Carboxyfluorescein; BHQ1, Black Hole Quencher 1; JOE, 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein; NA, not applicable.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Publisher’s note
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References
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Fernández-Soto P. Gandasegui Arahuetes J. Sánchez Hernández A. López Abán J. Vicente Santiago B. Muro A. (2014). A loop-mediated isothermal amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: a diagnostic approach in a murine model. PLoS Negl. Trop. Dis.8, e3126. 10.1371/journal.pntd.0003126
2
Gomes L. I. dos Marques L. H. S. Enk M. J. de Oliveira M. C. Coelho P. M. Z. Rabello A. (2010). Development and evaluation of a sensitive PCR-ELISA system for detection of Schistosoma infection in feces. PLoS Negl. Trop. Dis. 4, e664, 1–8. 10.1371/journal.pntd.0000664
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Gomes L. I. Marques L. H. S. Enk M. J. Coelho P. M. Z. Rabello A. (2009). Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples. Mem. Inst. Oswaldo Cruz104, 1194–1196. 10.1590/S0074-02762009000800021
4
Musso O. Sommer P. Drouet E. Cotte L. Neyra M. Grimaud J. A. et al . (1996). In situ detection of human cytomegalovirus DNA in gastrointestinal biopsies from AIDS patients by means of various PCR-derived methods. J. Virol. Methods56, 125–137. 10.1016/0166-0934(95)01892-1
5
Siqueira L. M. V. Senra C. de Oliveira A. A. de Carneiro N. F. Gomes L. I. Rabello A. et al . (2021). A real-time PCR assay for the diagnosis of intestinal schistosomiasis and cure assessment after the treatment of individuals with low parasite burden. Front. Immunol.11, 1–13. 10.3389/fimmu.2020.620417
Summary
Keywords
schistosomiasis, Schistosoma mansoni , diagnostic tests, diagnosis, latent class analysis, clinical research, sensitivity, specificity
Citation
Mesquita SG, Caldeira RL, Favre TC, Massara CL, Beck LCNH, Simões TC, Carvalho GBFd, Neves FGdS, de Oliveira G, Lacerda LdSB, de Almeida MA, Carvalho OdS, Mourão MM, Oliveira E, Silva-Pereira RA and Fonseca CT (2023) Corrigendum: Assessment of the accuracy of 11 different diagnostic tests for the detection of Schistosomiasis mansoni in individuals from a Brazilian area of low endemicity using latent class analysis. Front. Microbiol. 14:1331715. doi: 10.3389/fmicb.2023.1331715
Received
01 November 2023
Accepted
02 November 2023
Published
15 November 2023
Approved by
Frontiers Editorial Office, Frontiers Media SA, Switzerland
Volume
14 - 2023
Updates
Copyright
© 2023 Mesquita, Caldeira, Favre, Massara, Beck, Simões, Carvalho, Neves, de Oliveira, Lacerda, de Almeida, Carvalho, Mourão, Oliveira, Silva-Pereira and Fonseca.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Cristina Toscano Fonseca cristina.toscano@fiocruz.br
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.