ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1575257
This article is part of the Research TopicInfectious disease control in the microbial functional genomics eraView all 11 articles
Phylogenomic analysis of OXA-23-positive and genetic context view of rare NDM-1-producing carbapenem-resistant Acinetobacter baumannii isolates in one teaching hospital in China
Provisionally accepted
- 1Shaoxing Hospital of Traditional Chinese Medicine, Shaoxing, China
- 2Zhejiang Cancer Hospital, hangzhou, China
- 3Quzhou Maternal and Child Health Care Hospital, Quzhou, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Nosocomial outbreaks caused by carbapenem-resistant Acinetobacter baumannii (CRAB) strains are rapidly emerging worldwide and are cause for concern. Here, a phylogenetic tree of 18 A. baumannii strains collected from one teaching hospital in China was constructed to explore the genetic relationship from the genome insights with the aim to explore the relationship among them and observe the possible spread in the teaching hospital. All CRAB strains were collected from 17 patients and were mainly collected from sputum (55.56%, 10/18). Moreover, 61.11% (11/18) CRAB strains were collected from the ICU. Whole-genome sequencing (WGS) was performed on the Illumina and Oxford Nanopore platforms and the bioinformatic analysis was further performed. Based on the Pasteur MLST scheme, 16 A. baumannii strains were typed into a sequence type 2 (ST2). Two A. baumannii strains belong to two rare STs, named ST34 and ST23. KL and OCL analysis showed most of the strains (61.11%, 11/18) contained KL7. Whole-genome sequencing showed that 16 CRAB strains were OXA-23 producers and two other strains were found to carry blaNDM-1 and blaOXA-72. Genetic structure analysis showed the context of blaNDM-1 is ISAba14-aph(3')-VI-ISAba125-blaNDM-1-ble-MBL. Comparative genomics analysis showed 16 CRAB strains (ST2_Pas) have a close genetic relationship, and 8 CRAB strains possess a same resistance genes profile with only 1-6 SNPs were observed among these strains. Thus, there is an urgent need for increased surveillance in the clinics of patients and environment to prevent and control CRAB spreading.
Keywords: crab, WGS, Comparative genomics analysis, SNP, Plasmid structure
Received: 12 Feb 2025; Accepted: 22 Jul 2025.
Copyright: © 2025 Dong, Lou, Mao, Fang and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Su Dong, Shaoxing Hospital of Traditional Chinese Medicine, Shaoxing, China
Huan Zhang, Zhejiang Cancer Hospital, hangzhou, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.