ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Terrestrial Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1581725
This article is part of the Research TopicSoil Microbiome and Agroecosystem MultifunctionalityView all articles
Classification of Nostoc -like Morphology Cyanobacteria Isolated from Paddy Soil into Aliinostoc, Aulosira and Desmonostoc
Provisionally accepted
Hang T. L. Pham1*
Ngo Trang1
Thang V Tran1Tuan A. Duong2Long D. Tran1Anh T.T. Tran1Van T.H Nguyen1Sang V Nguyen1- 1University of Science, Vietnam National University, Hanoi, Hanoi, Vietnam
- 2University of Pretoria, Pretoria, South Africa
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Accurate identification of cyanobacterial strains is an essential step for subsequent research to be done on these organisms. The classification of cyanobacteria in Nostocaceae remains a significant challenge due to the lack of reference data for type species and robust morphological characters for each genus. This study aims to classify 38 new isolated Nostoc–like morphology strains to genus level. The relationship between phylogenetic classification and morphological characteristics at the genus level was also investigated. The 16S rRNA gene sequences served as primary data for phylogenetic classification, supporting the designation of eighteen isolates into the Aliinostoc, seven isolates into the Aulosira, and thirteen isolates into the Desmonostoc. Subsequently, we used these isolates as living materials to discover the most distinct features at each genus level of Aliinostoc, Aulosira, and Desmonostoc. As a result, the morphological characteristics of the three genera became distinguishable when grown in the BG110 medium. There, the mature vegetative cells of all isolated strains in the Aliinostoc were grey or brown , the strains in the Aulosira exhibited basal heterocysts at the beginning of cultivation, and the Desmonostoc strains showed the appearance of akinetes in the life cycle as alternative reproduction. All isolated strains exhibited heterocysts, indicating their ability to fix nitrogen and potentially improve nutrient availability in paddy soil, especially in nitrogen-deficient conditions. This study provides a dataset of 16S rRNA gene sequences and morphological characteristics of Nostoc morphotypes, contributing to cyanobacterial taxonomy.
Keywords: Aliinostoc, Aulosira, Desmonostoc, Cyanobacteria classification, Nostoc-like morphology, paddy soil, polyphasic approach
Received: 23 Feb 2025; Accepted: 28 Apr 2025.
Copyright: © 2025 Pham, Trang, Tran, Duong, Tran, Tran, Nguyen and Nguyen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Hang T. L. Pham, University of Science, Vietnam National University, Hanoi, Hanoi, 10000, Vietnam
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