Exploring the role of intestinal microbiota in mitigating acute radiation-induced intestinal injury through high-energy X-ray FLASH radiotherapy via metagenomic analysis

Huan Du¹Xiaofei Hao¹Binwei Lin¹Yihan Zhu¹Yiwei Yang²Mingming Tang³Wei Wu⁴Decai Wang¹Bo Lin¹Yuwen Li Ang³汤 文强³Haonan Xu³Jie Li¹Feng Gao¹Xiaobo Du^1*

• ¹Mianyang Central Hospital, Mianyang, China
• ²China Academy of Engineering Physics, Mianyang, China
• ³North Sichuan Medical College, Nanchong, Sichuan Province, China
• ⁴Southwest University of Science and Technology, Mianyang, Sichuan Province, China

Objectives This study preliminarily examines the potential correlation between the gut microbiome and the protective effects of FLASH radiotherapy (FLASH-RT) on intestinal tissue using metagenomic analysis. Methods Compact single high-energy X-ray source (CHEXs) FLASH-RT was employed for FLASH irradiation, while EBT3 radiochromic film and a fast current transformer were used to measure the absolute dose and the pulsed beam characteristics. Sham radiotherapy (control), FLASH-RT (333Gy/s), and Conventional dose rate radiotherapy (CONV-RT, 0.07Gy/s) were performed on whole abdomen of normal C57BL/6J female mice (10Gy,12Gy,14Gy). At 72 hours post-irradiation, intestinal contents from normal C57BL/6J female mice were collected for metagenomic analysis. The survival status, body weight, and damage to normal tissues were observed. Results At 28 days post-whole abdomen irradiation with doses of 12 Gy, the survival rate of the FLASH group was higher than that of the CONV group (p < 0.05). Histological analysis of intestinal tissues by H&E staining revealed significantly less acute intestinal damage and inflammation in the FLASH group compared to the CONV group. Further macrobiome analysis using LEfSe indicated that the abundance of beneficial bacteria, including Weissella, Lactobacillus ruminis and Lactobacillus taiwanensis was significantly higher in the FLASH group than in the CONV group. Moreover, compared to the CONV group, the FLASH group exhibited significant upregulation of several signaling pathways, including the Glycosaminoglycan degradation, PI3K/Akt and Arabinogalactan biosynthesis Mycobacterium signaling pathway. Conclusion Compared to CONV-RT, high-energy X-ray FLASH irradiation exerts radioprotective effects on normal intestinal tissue. Alterations in the gut microbiota and associated signaling pathways may be linked to the protective effects of FLASH.