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ORIGINAL RESEARCH article

Front. Microbiol.

Sec. Food Microbiology

Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1603412

This article is part of the Research TopicDiversity, Function, and Application of Microbes in the Fermentation or Production of Traditional FoodView all 17 articles

Composition and distribution characteristics of acetic acid related bacteria in the fermentation process of strong-flavor Baijiu

Provisionally accepted
Mandlaa  MandlaaMandlaa Mandlaa1*Mengyu  LiuMengyu Liu1Guojun  RenGuojun Ren2Hui  WangHui Wang2Fei  MaFei Ma2Hui  QuHui Qu2Min  ZhangMin Zhang2Jing  GuoJing Guo2Zhongjun  ChenZhongjun Chen1Ziyu  SunZiyu Sun1
  • 1Inner Mongolia Agricultural University, Hohhot, China
  • 2Inner Mongolia Hetao Liquor Group Co., Hohhot, China

The final, formatted version of the article will be published soon.

Ethyl acetate (EA) has an important impact on the quality of Strong-flavor Baijiu (SFB) and is synthesized from acetic acid (AA) and ethanol by microorganisms in the fermentation process. However, the composition and distribution characteristics of AA-related microorganisms is unclear in SFB production process. In this study the changes of bacterial community and concentration of AA during the fermentation process (7-67d) and different season fermentation (spring and summer) was investigated, and the composition of AA-related bacteria was analyzed to reveal the distributional characteristics of AA-related bacteria in brewing environment (Daqu, raw materials, pit mud, ground and tools). The results showed that the concentration of AA increased during the fermentation process and AA in summer was significantly higher than in spring (P<0.05). A total of 23 genera significantly related with AA (P<0.05) and most of them also significantly related (P<0.05) with the dominant genus, reducing sugar, moisture and acidity. Moreover, Pantoea (negatively related with AA) mainly come from DaquF and was the dominant genus in the fermentation process. Saccharopolyspora, Lactococcus and Streptomycetaceae, etc., the low abundance negative-related bacteria mainly came from DaquL and pit mud. Unclassified_Bacilli had positive relation with AA and mainly came from raw material. Ground and tools can provide 7 genera by low relative abundance.

Keywords: Strong-flavor baijiu, ethyl acetate, Acetic Acid, composition, source tracking, Bacteria

Received: 01 Apr 2025; Accepted: 02 Jul 2025.

Copyright: © 2025 Mandlaa, Liu, Ren, Wang, Ma, Qu, Zhang, Guo, Chen and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mandlaa Mandlaa, Inner Mongolia Agricultural University, Hohhot, China

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