ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1614122
This article is part of the Research TopicUnravelling Microbial Interactions in Plant Health and Disease DynamicsView all 18 articles
Delineating the Source of Resistance to Bean Common Mosaic Virus (BCMV) and Bean Common Mosaic Necrosis Virus (BCMNV) in Common Bean (Phaseolus vulgaris) Cultivars of Jammu & Kashmir, A North-Western Himalayan Region
Provisionally accepted- Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, India
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Bean common mosaic virus (BCMV) and bBean common mosaic necrosis virus (BCMNV) are among the most challenging constraints for common bean production in Northern states of India due to their easy transmission through aphids and seeds. Highly valuable Indian common bean varieties and landraces are more susceptible to BCMV and BCMNV and very few varieties exhibit resistance to these viruses. Resistance towards these viruses is governed by a single dominant (I) gene and a few recessive genes (bc-1, bc-2, bc-3, bc-4, bc-u d and bc-u r ). This study aims to identify common bean genotypes bearing multiple resistant genes, each working with a different mode of action. A total of 123 genotypes of common beans were mechanically inoculated with BCMV and BCMNV isolates and molecular markers (SW13, ROC11, BCMV-CAPS, ENM-FWe/Rve) were used to identify the presence of these two resistant genes (I and bc-3). Out of these, 23 genotypes were found identified plant materials conferring phenotypically resistantce to both viruses.to the virus were resown and re-confirmed by inoculating the same set of isolates used earlier. The virus accumulation in the resistant genotypes was also understood properly through a time course experiment in a qPCR assay. Furthermore, molecular screening was performed in which total of 13 hypersensitive resistant genotypes bearing a single dominant gene (I) were confirmed through SW13 and BCMV-CAPS markers. Additionally, ROC11/420, ENMF/R markers identified 4 genotypes bearing the recessive (bc-3) gene conferring complete resistance to the virus without executing hypersensitive response (HR). A valuable gene combination of both I, bc-3 (Ibc-3, Host group-12) genes in 3 genotypes was also established in the screened germplasm. However, in 3 phenotypically resistant genotypes, neither I gene nor bc-3 gene was identified. The virus accumulation in the resistant genotypes was also understood properly through a time course experiment in a qPCR assay. This extensive identification of resistant common bean genotypes against BCMV and BCMNV can be readily included in the common bean breeding program of the Northern states of India for virus resistance.
Keywords: BCMV, BCMNV, Resistance, i-gene, bc-3 gene Font: Italic Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: Italic Font: Not Bold, Italic Font: Not Bold, Italic
Received: 18 Apr 2025; Accepted: 30 May 2025.
Copyright: © 2025 Meghanath, Wani, Bashir, Rashid, Javaid, Dar, Wani, Sofi, Ali and WANI. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: AFLAQ HAMID WANI, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, India
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