ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
This article is part of the Research TopicNext-Generation Technologies for Antibiotic Susceptibility TestingView all 9 articles
Rapid Colistin Susceptibility Determination by a Novel Fluorogenic Method in Klebsiella pneumoniae Strains
Provisionally accepted
- 1Department of Medical Biotechnology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University,, Istanbul, Türkiye
- 2Department of Medical Laboratory Techniques ,Vocational School of Health Services, Acibadem Mehmet Ali Aydinlar University, Istanbul, Türkiye
- 3Department of Medical Microbiology, School of Medicine, Acıbadem Mehmet Ali Aydınlar University,, Istanbul, Türkiye
- 4Acıbadem University, Istanbul, Türkiye
- 5Department of Medical Microbiology, School of Medicine, Acıbadem Mehmet Ali Aydınlar University,, Istanbul,, Türkiye
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Introduction: Klebsiella pneumoniae is a causative agent that can lead to fatal infections, especially in intensive care patients. Colistin treatment is employed as a last resort in K. pneumoniae infections caused by strains resistant to almost all antibiotics from different groups. However, in recent years, colistin resistance has also become widespread in K. pneumoniae strains. Therefore, before starting treatment with this relatively toxic drug, it is essential to know its susceptibility to colistin. A standard colistin susceptibility test typically takes 24 to 48 hours. In this study, we investigated the accuracy of our novel fluorometric method, which enables the determination of colistin minimal inhibitory concentration (MIC) within two hours for K. pneumoniae. Methods: For this purpose, we determined and compared the MIC values of 24 colistin-resistant and 18 colistin-susceptible K. pneumoniae strains using the standard broth microdilution method and the new fluorometric method. Results: In the comparison of MIC values determined by standard and fluorometric methods, it was observed that the MIC values of 22 strains were identical, there was a 1-dilution (1/2) difference in 14 strains, and a 2-dilution (1/4) difference in 6 strains. Discussion: We believe that the new fluorometric antibiotic susceptibility testing method will be a valuable tool in the rapid detection of colistin resistance in K. pneumoniae and thus for guiding appropriate treatment in critically ill patients.
Keywords: Colistin resistance, Fluorometric assay, Klebsiella pneumoniae, minimum inhibitory concentration (MIC), Rapid antimicrobial susceptibility testing
Received: 26 Aug 2025; Accepted: 10 Dec 2025.
Copyright: © 2025 Manteghi, Ayas, Akyar, Mozioglu and KOCAGOZ. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Erkan Mozioglu
TANIL KOCAGOZ
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