Translating in vitro gut microbiota models to human context: compositional correlations under dietary fibre intervention

Femke Hoevenaars^1*Torsten Pascal Marcel Scheithauer¹Boukje Eveleens Maarse²Isabela Fernandes de Oliveira¹Ines Warnke³Wilbert Sybesma⁴Matthijs Moerland²Tim van den Broek¹Frank Schuren¹

• ¹TNO, Leiden, Netherlands
• ²Stichting Centre for Human Drug Research, Leiden, Netherlands
• ³DSM Werk Sisseln, Sisseln, Switzerland
• ⁴Microbiome Solutions, Niederhünigen, Switzerland

Large interindividual variation in human gut microbiota composition and its response to interventions limits the development of novel microbiota-targeted supplements. In vitro models reflecting this interindividual variation and predicting individual in vivo microbiota responses would allow for the assessment of the potential efficacy of such interventions. Here, we investigated whether in vitro microbiota modulation by a dietary fiber mixture is translatable to in vivo microbiota outcomes. A 12-week double blind, randomized, placebo-controlled, crossover study with a dietary fiber mixture of acacia gum (AG) and carrot powder was performed in healthy volunteers (N = 54, 45-70 years, BMI 27.3 ± 1.4 kg/m2). A significant effect on microbiota composition was shown in vivo, although with strong individual variation. The fiber intervention was mimicked in vitro by exposing each individuals' baseline microbiota to the same dietary fiber as used for the 12-weeks in vivo intervention. A significant correlation was shown between the in vitro and human fecal microbiota composition after 8-and 12-weeks intervention (p = 0.003 and p = 0.0107, respectively). Microbial taxa responding to the intervention in vitro and in vivo also showed clear overlap (p = 0.002). These results demonstrate potential for pre-study selection of donors whose microbiotas respond to specific intervention.