ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Virology
This article is part of the Research TopicViral Diagnostics: Advancements for Rapid and Enhanced DetectionView all 4 articles
Development of Immunogold Test Strips for Chili Veinal Mottle Virus Detection Using Monoclonal Antibodies Against the CP
Provisionally accepted
- 1Anhui Academy of Agricultural Sciences (CAAS), Hefei, China
- 2Anhui Province Key Laboratory of Pesticide Resistance Management on Grain and Vegetable Pests, 230031, Hefei, China, hefei, China
- 3Institute of Luzhou Tobacco Company of Sichuan Province, 646000, Luzhou, China, Luzhou, China
- 4Xuancheng City Xuanzhou District Tobacco Industry Development Center, 242000, Xuancheng, China, xuancheng, China
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Chilli veinal mottle virus (ChiVMV), a member of the Potyviridae family, a major threat to solanaceous crops, including tobacco and peppers, in China, where it causes systemic necrosis, leaf mottling, and significant yield losses. Early and accurate detection is essential for effective disease management, yet conventional assays such as RT-PCR and ELISA are constrained by laboratory requirements and skilled personnel. In this study, we developed a colloidal gold-based immunochromatographic strip (ICS) for the rapid, specific, and field-deployable detection of ChiVMV. The viral coat protein (CP) was heterologously expressed in Escherichia coli and used to generate eight hybridoma cell lines, from which two monoclonal antibodies, 22F4 (capture) and 45C4 (detector), were selected. The assembled ICS yielded clear visual results within 5–10 minutes, with a detection limit corresponding to a 1:1000 dilution of infected leaf extracts, and exhibited no cross-reactivity with PVY, TVBMV, WYMV, TMV, or CMV. Field testing with 120 tobacco samples demonstrated 95.7% sensitivity and 98.0% specificity, consistent with RT-PCR, and the test strips maintained stability after 12 months of storage at room temperature. This study presents the first rapid ICS for ChiVMV, providing a sensitive, specific, and cost-effective diagnostic tool for on-site disease surveillance and early management in tobacco cultivation.
Keywords: monoclonal antibodies, Immunochromatographic strip, chilli veinal mottle virusttobacco plants, Sandwich, Chilli veinal mottle virus
Received: 09 Oct 2025; Accepted: 29 Nov 2025.
Copyright: © 2025 Wang, Xu, Chen, Wang, An and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Guo Ben Zhou
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