ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbiotechnology
The Regulatory Role of Histidine Kinase in Modulating Nucleoside Metabolites in Streptomyces noursei CK-15
Provisionally accepted
- Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
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Histidine kinase, a vital component of the two-component system, regulates the synthesis of secondary metabolites and is integral to key processes related to the growth and environmental adaptation of Streptomyces. The SHK gene may govern the manufacture of the nucleoside-type secondary metabolites Toyocamycin and Wuyiencin. This study generated a histidine kinase overexpression mutant strain (S. noursei oSHK) exploiting ΔtoyG transcription data. This mutant strain was seen to suppress the growth of the CK-15 strain, resulting in a 25.94% reduction in Wuyiencin levels and a 17.64% decrease in Toyocamycin levels, while also decreasing spore formation and hyphal development rate. The SHK gene demonstrates reduced efficacy against bacteria and compromised nitrogen use, suggesting a detrimental regulatory influence on strain viability and secondary metabolite synthesis in S. noursei CK-15. This study provides theoretical justification for the modification of high-yielding Wuyiencin strains.
Keywords: biosynthesis, competition, Gene clusters, Toyocamycin, Wuyiencin
Received: 26 Aug 2025; Accepted: 07 Jan 2026.
Copyright: © 2026 Liao, Xie, Zhao, Yu and Ge. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Beibei Ge
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