ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Virology
HIV-1 drug resistance among HIV/HCV co-infected patients with treatment failure in Yunnan, southwestern China: a cross-sectional study
Min Zhong 1
Huan Li 1
xiaobo ma 1
Li Ya 1
Chenglu He 1
Fang Zou 1
Xilin Kang 2
1. The First Affiliated Hospital of Kunming Medical University, Kunming, China
2. Yan'an Hospital Affiliated To Kunming Medical University, Kunming, China
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Abstract
Background Yunnan Province remains a region with a high prevalence of human immunodeficiency virus (HIV) in China. Due to shared transmission routes, HIV/Hepatitis C virus (HCV) co-infection is common. This study aimed to analyze the prevalence and mutation patterns of HIV-1 drug resistance among HIV/HCV co-infected patients specifically following the virological failure of first-line highly active antiretroviral therapy (HAART) in Yunnan, and to compare these characteristics with HIV-1 mono-infected patients. Methods A cross-sectional study was conducted among 104 HIV/HCV co-infected and 120 HIV-1 mono-infected patients who experienced virological failure (HIV-RNA \geq 1,000 copies/mL) after at least 6 months of ART. Genotypic drug resistance was tested using an in-house method and analyzed via the Stanford HIV drug resistance database. Multivariable logistic regression and stratified analysis were performed to adjust for confounders. Results Among patients with treatment failure, the drug resistance rate in the HIV/HCV co-infection group (37.5%) was significantly lower than in the HIV-1 mono-infection group (55.0%, P = 0.009). Multivariable logistic regression showed that HIV/HCV co-infection was associated with a lower trend of resistance, although it did not reach formal statistical significance after adjusting for gender, treatment duration, and CD4+ Count (aOR = 0.49, P = 0.084). However, stratified analysis revealed that co-infection was significantly associated with lower resistance in patients with a treatment duration of 6-12 months (OR = 0.24, P = 0.001) and those with CD4+ Count ≤350 cells/μL (OR = 0.38, P = 0.001). The frequency of the NRTI-related mutation T69D/N/S was significantly lower in the co-infected group (P = 0.029). Conclusions Among patients experiencing virological failure, HIV/HCV co-infection is associated with distinct genotypic resistance profiles, particularly in the early stages of treatment failure and among immunodeficient individuals. These findings suggest that co-infection status may influence the pathway to HIV drug resistance. Clinicians should prioritize prompt genotype resistance testing for co-infected patients failing ART to optimize second-line regimen adjustments.
Summary
Keywords
Drug Resistance, Genotype, Highly active antiretroviral treatment, HIV - human immunodeficiency virus, HIV/HCV co-infection
Received
19 February 2026
Accepted
19 February 2026
Copyright
© 2026 Zhong, Li, ma, Ya, He, Zou and Kang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Min Zhong
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